Fermented feed

A technology for fermenting feed and raw materials, applied in animal feed, animal feed, microorganisms, etc., can solve the problems of lack of cellulase, incomplete enzyme system, insufficient enzyme amount, etc., to inhibit reproduction, improve digestion and absorption rate, and improve digestion capacity Effect

Active Publication Date: 2011-11-02
山东省明发同茂饲料有限公司
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Problems solved by technology

This low utilization rate is mainly caused by the following two reasons: ①The animal's own enzyme system is incomplete,...

Method used

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Embodiment ( 1

[0018] 1. Materials

[0019] 1.1 Strains

[0020] Lactobacillus acidophilus ACCC10637, Candida utilis ACCC20060, and Bacillus subtilis ACCC10619 were purchased from China Agricultural Microorganism Culture Collection Center.

[0021] 1.2 Strain Activation and Shake Flask Medium

[0022] Lactobacillus acidophilus ACCC10637: Activated with MRS agar medium and rejuvenated, made shake flask seeds with MRS liquid medium.

[0023] Candida utilis ACCC20060: Activated with YPD agar medium and rejuvenated, made shake flask seeds with wort medium.

[0024] Bacillus subtilis ACCC10619: Activated with LB agar medium and rejuvenated, made shake flask seeds with LB liquid medium.

[0025] 1.3 Strain production medium:

[0026] Soybean whey was used as the raw material of the liquid medium to expand the culture of Lactobacillus acidophilus ACCC10637 and Bacillus subtilis ACCC10619 respectively, and the inoculum size was 10%. The culture conditions were: temperature 37°C, mixer speed 100r...

Embodiment ( 2

[0035] Select 96 healthy piglets with similar age and body weight (18 ± 1) kg, and randomly divide them into two groups, namely, the experimental group and the control group, with 48 piglets in each group, 4 replicates in each group, and 4 replicates in each replicate. 12 heads, half male and half female in each group. The test group was fed with ordinary full-price feed, and the test group added fermented feed to the ordinary full-price feed at a rate of 10% (that is, 1 kg of fermented feed was added to every 9 kg of ordinary full-price feed). The test time of each stage is 34 days for small pigs, 35 days for medium pigs and 28 days for large pigs.

[0036] During the 7 days of the pre-test period, the pigs were observed, immunized, driven out and ear-numbered, and the test materials were gradually transferred. Entering the test period, the two test groups were fed with the corresponding test rations, fed twice a day, and the pigs had free access to food and water. Clean the ...

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Abstract

The invention discloses a fermented feed. The fermented feed is prepared by the following steps of: performing amplification culture on Lactobacillus acidophilus ACCC10637 and Bacillus subtilis ACCC10619 respectively to obtain amplification culture solution by taking soybean whey as a raw material of a liquid culture medium, and performing amplification culture on Candida utilis ACCC20060 to obtain an amplification culture medium by a semi-solid culture medium; and according to the weight of a semi-solid-state fermentation culture medium, adding 3.2 percent of amplification culture solution of the Lactobacillus acidophilus ACCC10637, 1.0 percent of amplification culture medium of the Candida utilis ACCC20060, and 0.1 percent of amplification culture solution of the Bacillus subtilis ACCC10619 into the semi-solid-state fermentation culture medium respectively, and sealing and fermenting for 36 to 72 hours at the temperature of between 28 and 32 DEG C. When 10 percent of fermented feed is added into a pig feed, the utilization rate of the feed can be improved by 7 to 8 percent, the morbidity is reduced by 8 to 10 percent, and the culture environment is remarkably improved.

Description

technical field [0001] The invention relates to a fermented feed, which belongs to the technical field of feed. Background technique [0002] As early as 100 years ago, Nobel Prize-winning Russian bacteriologist Elie Metchnikoff proposed that bacteria in fermented dairy products may be used to control the fermentation process of human intestinal bacteria, thereby promoting health role. Today, people can buy yogurt, which contains probiotics. [0003] In the past 10 years, the concept of probiotics has also gained application in animal nutrition. Microbes, given orally in sufficient doses, alter the gut microbiota and have beneficial effects on the host. Summarizing several published trials, most results show that probiotics can play a growth-promoting role. However, in feeding trials, only a part of the significant promotion effect was observed. This shows that there are great differences in the responses of different individual animals to this feed additive. Probiotic...

Claims

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Application Information

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IPC IPC(8): A23K1/00C12N1/16C12N1/20C12R1/23C12R1/125C12R1/72A23K10/12
Inventor 李学东
Owner 山东省明发同茂饲料有限公司
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