Method for purifying mariculture wastewater by utilizing compound microbial inoculant

A compound microbial agent and mariculture technology, applied in biological water/sewage treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve the problem of ineffective removal of phosphorus and other pollutants and single treatment bacteria , Incomplete and other problems, to achieve the effect of economic feasibility, improvement of breeding quality and obvious effect

Inactive Publication Date: 2012-03-21
QUANZHOU NORMAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method mainly removes nitrate in the water body, and the removal effect on organic matter, phosphorus and other pollutants is not obvious, and the treatment bacteria are single and not thorough.

Method used

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  • Method for purifying mariculture wastewater by utilizing compound microbial inoculant
  • Method for purifying mariculture wastewater by utilizing compound microbial inoculant
  • Method for purifying mariculture wastewater by utilizing compound microbial inoculant

Examples

Experimental program
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Effect test

Embodiment 1

[0016] In this example, Rhodopseudomonas palustris of CCTCC M 205147, Arthrobacter of ACCC 10691, Alcaligenes denitrificans of ACCC 03247, Lactobacillus acidophilus of ACCC 10637, and Utilization of CGMCC 2.1016 were used in this example. Candida and Bacillus subtilis of CGMCC 1.1630 are domesticated for salt tolerance and temperature tolerance, and are used as production bacteria to form a composite microbial agent. Among them, the total number of microorganisms in the compound microbial agent is ≥1.0×10 10 CFU / g, and photosynthetic bacteria ≥5.0×10 8 CFU / g, nitrifying bacteria≥2.0×10 8 CFU / g, denitrifying bacteria ≥3.0×10 8 CFU / g, acidophilus ≥1.0×10 9 CFU / g, Candida utilis ≥5.0×10 8 CFU / g, Bacillus ≥7.5×10 9 CFU / g.

[0017] The steps of salt-tolerant breeding of strains are as follows. The selected strains are cultivated in the gradient medium containing 0.3%-2% sodium chloride to gradually improve the salt tolerance of the strains. After several generations of salt...

Embodiment 2

[0022] In this example, Rhodopseudomonas palustris of CCTCC M 205147, Arthrobacter of ACCC 10691, Alcaligenes denitrificans of ACCC 03247, Lactobacillus acidophilus of ACCC 10637, and Utilization of CGMCC 2.1016 were used in this example. Candida and Bacillus subtilis of CGMCC 1.1630 are subjected to salt-tolerant and temperature-resistant acclimatization (conditions are the same as in Example 1), and are used as production bacteria to form a composite microbial bacterial agent. Among them, the total number of microorganisms in the compound microbial agent is ≥1.0×10 10 CFU / g, and photosynthetic bacteria ≥5.0×10 8 CFU / g, nitrifying bacteria≥2.0×10 8 CFU / g, denitrifying bacteria ≥3.0×10 8 CFU / g, acidophilus ≥1.0×10 9 CFU / g, Candida utilis ≥5.0×10 8 CFU / g, Bacillus ≥7.5×10 9 CFU / g.

[0023] Medium activation is used, and the medium components are: peptone 3g / L, yeast powder 3g / L, glucose 5g / L, sodium chloride 10g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate ...

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Abstract

The invention discloses a method for purifying mariculture wastewater by utilizing a compound microbial inoculant. The method comprises the following steps: (1) screening the compound microbial inoculant which consists of photosynthetic bacteria, nitrobacteria, denitrifying bacteria, candida utilis, lactobacillus acidophilus and bacillus; (2) activating the compound microbial inoculant by utilizing a culture medium: directly adding the strain into the culture medium based on 1% of inoculation amount (w/v), and activating the strain by a shaker at the temperature of 37 DEG C with the speed of 150rpm (revolution per minute) for 24 hours so that the total content of microbes is 7*10<8>-9*10<8>CFUs (colony forming units)/mL; and (3) adding the activated compound inoculant into the treated aquaculture wastewater, wherein the compound inoculant accounts for 0.06%-0.12% of the total weight of the aquaculture wastewater. The method has the advantages of no toxicity and harm on aquaculture animals, simple process, fast organic matter degradation speed, obvious effect, no drug residues and no secondary pollution. Therefore, the method has economic feasibility and is applicable to popularization and application to coastal aquaculture industry.

Description

technical field [0001] The invention relates to a method for purifying marine aquaculture wastewater by using a compound microbial bacterial agent. Background technique [0002] In recent years, the rapid development of my country's mariculture industry has driven the rapid development of the economy, but it has also had a great impact on the coastal ecosystem. Due to the defects of aquaculture's own ecological structure and farming methods, the mariculture industry has produced a series of serious impacts on the marine ecosystem, such as nutrient pollution, changes in the depositional environment, pollution from drug use, and impacts on coastal organisms. The aquaculture wastewater contains high concentrations of organic matter, nitrogen, phosphorus, etc., and the water quality is eutrophic, which has a great poisonous effect on aquaculture fish. The environmental pollution of coastal mariculture is deteriorating day by day, which seriously affects the ecological safety of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
Inventor 袁建军陈翠雪卢英华
Owner QUANZHOU NORMAL UNIV
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