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Method for extracting high-purity chlorogenic acid from honey suckle flower

A technology of honeysuckle and chlorogenic acid, applied in the separation/purification of carboxylate, organic chemistry, etc., can solve problems such as difficulty in filtration, achieve uniform particle size distribution, facilitate the extraction of active ingredients, and reduce consumption.

Inactive Publication Date: 2010-10-06
湖南济草堂金银花科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for extracting high-purity chlorogenic acid from Flos Lonicerae, to solve the difficulty in filtering the current chlorogenic acid extract due to the large amount of precipitation in the process of removing impurities and improving the product quality. Purity and Yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for extracting high-purity chlorogenic acid from honeysuckle, comprising the steps of:

[0021] (1) Pulverization: collect honeysuckle from July to October, dry, and dry the water content ≤ 10%, put the honeysuckle material into a stainless steel airtight container, and freeze it with liquid nitrogen to reduce the temperature of the honeysuckle material The glass is in a state of embrittlement at -196°C; then a supersonic nitrogen gas flow is introduced into the airtight container, so that the low-temperature honeysuckle material is quickly crushed under the impact and friction of the supersonic flow, and cell-level honeysuckle powder is obtained;

[0022] (2) Enzyme treatment: add deionized water with pH=5.5 to the honeysuckle micropowder and stir evenly, soak for 1 hour, the amount of deionized water added is 15 times of the weight of the honeysuckle micropowder; then add exoglucan Put the carbohydrase into an airtight container and stir evenly; the amount of...

Embodiment 2

[0029] A method for extracting high-purity chlorogenic acid from honeysuckle, comprising the steps of:

[0030] (1) Pulverization: collect honeysuckle from July to October, dry, and dry the water content ≤ 10%, put the honeysuckle material into a stainless steel airtight container, and freeze it with liquid nitrogen to reduce the temperature of the honeysuckle material The glass is in a state of embrittlement at -196°C; then a supersonic nitrogen gas flow is introduced into the airtight container, so that the low-temperature honeysuckle material is quickly crushed under the impact and friction of the supersonic flow, and cell-level honeysuckle powder is obtained;

[0031](2) Enzyme treatment: add deionized water with pH=5.8 to the honeysuckle micropowder and stir evenly, soak for 1 hour, the amount of deionized water added is 10 times of the weight of the honeysuckle micropowder; add exoglucan to the soaking solution Put the carbohydrase into an airtight container, and stir ev...

Embodiment 3

[0038] A method for extracting high-purity chlorogenic acid from honeysuckle, comprising the steps of:

[0039] (1) Pulverization: collect honeysuckle from July to October, dry, and dry the water content ≤ 10%, put the honeysuckle material into a stainless steel airtight container, and freeze it with liquid nitrogen to reduce the temperature of the honeysuckle material The glass is in a state of embrittlement at -196°C; then a supersonic nitrogen gas flow is introduced into the airtight container, so that the low-temperature honeysuckle material is quickly crushed under the impact and friction of the supersonic flow, and cell-level honeysuckle powder is obtained;

[0040] (2) Enzyme treatment: add deionized water with pH=6 to the honeysuckle micropowder and stir evenly, soak for 1 hour, the amount of deionized water added is 12 times of the weight of the honeysuckle micropowder; then add exoglucan Put the carbohydrase into an airtight container and stir evenly; the amount of e...

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Abstract

The invention discloses a method for extracting high-purity chlorogenic acid from honey suckle flower. The technical scheme of the invention comprises the following steps of: firstly, smashing the honey suckle flower material by the low-temperature supersonic air stream to obtain the cell-level honey suckle flower micro powder; secondly, carrying out the enzyme treatment, ultrasonic treatment, water extraction and filtering on the cell-level honey suckle flower micro powder to obtain the initial solution of the chlorogenic acid; and finally, flocculating, decolouring, purifying, condensing and drying the initial solution to obtain the high-purity chlorogenic acid. The invention solves the problems that the chlorogenic acid extract of the prior art is difficult to filter due to the large quantity of the precipitate in the impurity-removal and purification process; the invention has the advantages of advanced technique, simple and convenient operation, low cost, no harmful solvent, highpurity, high yielding rate, suitability for industrial large-mass production and the like.

Description

technical field [0001] The invention discloses a method for extracting high-purity chlorogenic acid from honeysuckle. Background technique [0002] Honeysuckle is the flower bud of Lonicera japonicaThund, a perennial semi-evergreen winding woody vine of the Lonicera family. It contains luteolin, isochlorogenic acid, chlorogenic acid, inositol, volatile oil and other ingredients. The main component of honeysuckle extract is chlorogenic acid, accounting for about 98-99% of the total components. Chlorogenic acid alias: coffee tannic acid; system name: 1,3,4,5-tetrahydroxycyclohexanecarboxylic acid-(3,4-dihydroxycinnamate); belongs to caffeoylquinic acid derivatives. Properties: white fine powder, melting point 205-209℃; solubility: good water solubility, easily soluble in hot water, ethanol and acetone, very slightly soluble in ethyl acetate, insoluble in petroleum ether; molecular formula and molecular weight: C 16 h 18 o 9 =354.30; Biological activity: Chlorogenic acid is...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48
Inventor 刘源王红刘凌航戴艳平刘纯一
Owner 湖南济草堂金银花科技开发有限公司
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