Method for extracting chlorogenic acid from Eucommia leaves and use thereof

A technology of eucommia ulmoides leaves and chlorogenic acid, which is applied in the fields of application, medical preparations containing active ingredients, and pharmaceutical formulas, which can solve the problems of reducing feed utilization and achieve the effects of low cost, no harmful solvents, and simple process operation

Inactive Publication Date: 2009-07-22
江苏省苏微微生物研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This may be due to the flavonoids in the active ingredients of Eucommia can inhibit the activity of many enzymes (see "Foreign Medicine" 1998 Peng Fang "Biological Activity of Flavonoids") can inhibit the growth of animals and reduce the utilization of their feed Rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The method for extracting chlorogenic acid from Eucommia leaves comprises the following steps:

[0020] 1.1.1 Pulverization: collect eucommia leaves from July to October, dry them, and dry them until the moisture content is ≤10%. Grind the dried eucommia leaves and pass through a 80-mesh sieve to obtain 1000g of eucommia leaf powder;

[0021] 1.1.2 Enzyme treatment: Add 5g of plant cellulase with an activity of 500-1000U / g to the Eucommia leaf powder and put it in a closed container, then add 10Kg of deionized water with a pH of 4.5 in the closed container and stir evenly. Control the temperature of the mixture to 40°C and soak at a constant temperature for more than 20 hours;

[0022] 1.1.3 Ultrasonic treatment: wave the enzyme-treated mixture for 30 minutes at room temperature and at an ultrasonic frequency of 50KHz;

[0023] 1.1.4 Filtration: filter the filtrate from the ultrasonically treated mixture with a high-speed centrifuge to obtain 1480 g of filter residue; ...

Embodiment 2

[0028] The production process is as described in Example 1, except that fresh Eucommia leaves are used to obtain a chlorogenic acid content of 90.5%.

Embodiment 3

[0030] The production process is as described in Example 1, except that the cellulase content is 1%, and the obtained chlorogenic acid content is 92.7%.

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PUM

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Abstract

The invention discloses a method for extracting chlorogenic acid from folium cortex eucommiae. The invention also discloses an application of the chlorogenic acid in prevention substances of fish diseases. The method comprises the steps of grinding, enzyme treatment, ultrasonication, filtering, extraction, concentration, purification, and the like; the method has the advantages of simple and convenient technique operation, low cost, no hazardous solvent, high yield, being applicable to industrialized production, and the like; in addition, the chlorogenic acid has good bacteriostasis effect on pathogenic bacteria commonly seen in aquiculture, with a bacteriostasis effect on aeromonas hydrophila equal to that of antibacterials like antibiotics, can strengthen the immunity of the organism and has relatively strong prevention effect on bacterial diseases.

Description

technical field [0001] The invention discloses a method for extracting chlorogenic acid from Eucommia ulmoides leaves, and also discloses the application of chlorogenic acid in fish disease prevention and control substances. Background technique [0002] Eucommia ulmoides is a traditional Chinese medicinal material with a history of more than 2,000 years in my country. Its functions are to nourish the liver and kidney, strengthen bones and muscles, and prevent miscarriage. At the same time, Eucommia ulmoides has special effects on treating hypertension. Chlorogenic acid is one of the main active components of Eucommia leaves, which has significant physiological activity. Chlorogenic acid is a derivative of caffeoylquinic acid, which has various pharmacological effects such as choleretic, antibacterial, antiviral, blood pressure lowering, increasing white blood cells and stimulating the central nervous system. It is an important raw material for health food, medicine, cosmeti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732A23K1/17A61K31/216A61P31/04C12S3/00A23K20/195
Inventor 张东升张凌裳赵春城周群兰谢俊赵晓联杜郭君王红连秦海萍蔡建荣蔡正森龚燕
Owner 江苏省苏微微生物研究有限公司
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