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Method for preparing chlorogenic acid in Eucommia ulmoides leaves

A technology of Eucommia ulmoides leaves and chlorogenic acid, which is applied in chemical instruments and methods, separation/purification of carboxylate, preparation of carboxylate, etc. It can solve the problems of low product purity, large amount of waste water, low extraction rate of chlorogenic acid, etc. problems, to achieve the effect of simple membrane operation process, low water extraction temperature, and long-term stability of membrane flux

Inactive Publication Date: 2011-05-04
HUBEI LAOLONGDONG DUZHONG DEV
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  • Description
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  • Application Information

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Problems solved by technology

[0004] In order to overcome the above-mentioned problems and shortcomings in the prior art, the present invention provides a method for preparing chlorogenic acid in Eucommia leaves, which is to add cellulase and medium-temperature water to pulverized Eucommia leaves powder Extract chlorogenic acid from Eucommia ulmoides leaves, then separate and purify the extracted chlorogenic acid through two-stage membrane separation technology combined with resin adsorption elution process, collect ethanol eluate, concentrate under reduced pressure, and vacuum dry to obtain product chlorogenic acid. Effectively solve the problems of low extraction rate, large amount of waste water and low product purity in the original production of chlorogenic acid

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  • Method for preparing chlorogenic acid in Eucommia ulmoides leaves
  • Method for preparing chlorogenic acid in Eucommia ulmoides leaves

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Embodiment Construction

[0024] Example 1, see the attached figure 1 , take 10Kg of dried Eucommia leaves, crush them and pass through a 200-mesh sieve, add 120Kg of water, adjust the temperature to 55°C, keep the temperature constant and adjust the pH value to 4.5, then add 10g of cellulase, extract for 60min under this condition, filter, and filter the residue again Extract for the second time according to the aforementioned extraction conditions, combine the two filtrates, and filter conventionally, the extraction rate of chlorogenic acid is 95.8%; the operating conditions are P=0.3Mpa, T=45°C, PH=4.5, Q=70L / min filtrate After passing through a 0.03μm ultrafiltration membrane, the extract was concentrated 11.2 times under this condition, and the average flux of the ultrafiltration membrane was 226.3L / m 2 h; under the operating conditions P=1.5Mpa, T=50°C, PH=4.5, Q=20L / min, the ultrafiltration filtrate is passed through a 200 molecular weight nanofiltration membrane, and the ultrafiltration filtrat...

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Abstract

The invention discloses a method for preparing chlorogenic acid in Eucommia ulmoides leaves, which comprises the following steps of: crushing the Eucommia ulmoides leaves, performing warm water extraction assisted by cellulose, performing conventional filtration, removing impurities by using an ultrafiltration membrane, performing nanofiltration and concentration, separating and purifying by using macroporous resin, and concentrating and drying to obtain chlorogenic acid powder. The chlorogenic acid in the Eucommia ulmoides leaves is subjected to warm water extraction assisted by cellulose, the extraction rate can reach 95 percent, the water extraction temperature is lower, and the extraction time is shortened; the ultrafiltration membrane is adopted to remove the impurities from the extracting solution and separate other byproducts, the membrane operating process is simple, the membrane flux can be stable for a long time, and the membrane is easy to clean, has long service life and is suitable for industrialization; a nanofiltration membrane is adopted to concentrate the extracting solution from which the impurities are removed, the load of the subsequent process is lightened, and the service life of the resin can be prolonged; the filtrate obtained through nanofiltration has the chemical oxygen demand (COD) value of less than or equal to 100 and can be returned to the production process, and wastewater is not generated basically; and the macroporous adsorbent resin can effectively purify the chlorogenic acid, the purity of the chlorogenic acid in the prepared powder is more than 60 percent and the extraction ratio is more than 1.4 percent.

Description

technical field [0001] The invention relates to a method for preparing chlorogenic acid in leaves of Eucommia ulmoides, in particular to a method for preparing chlorogenic acid by adopting an enzymatic medium-warm water extraction, membrane separation technology and resin technology. Background technique [0002] Chlorogenic acid is an important biologically active substance, which has multiple pharmacological effects such as choleretic, antibacterial, antiviral, blood pressure lowering, blood lipid lowering, free radical scavenging, antitumor, white blood cell increase, and central nervous system excitement. It is widely used in the field of health care products. Chlorogenic acid is mainly found in natural plants such as Eucommia ulmoides, honeysuckle, and coffee beans. Eucommia ulmoides is a unique economic tree species in my country, with a large distribution area and rich resources. The content of chlorogenic acid in Eucommia ulmoides leaves is 1-5.5%, which is an ideal...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48C07C67/56B01D15/08
Inventor 徐斌万瑞极彭小文王金廉志清刘冬成刘芸芳王震
Owner HUBEI LAOLONGDONG DUZHONG DEV
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