Method for preparing chlorogenic acid from eucommia leaves

A technology of eucommia ulmoides leaves and chlorogenic acid, which is applied in the preparation of carboxylic acid esters, chemical instruments and methods, and the preparation of organic compounds, and can solve the problems of low product purity, large amount of waste water, and low extraction rate of chlorogenic acid, and achieve The effect of large adsorption capacity, low water extraction temperature, and long-term stability of membrane flux

Active Publication Date: 2014-03-19
PERFECT CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the existing problems and shortcomings of the prior art, the present invention provides a method for preparing chlorogenic acid from Eucommia leaves, which effectively solves the problems of low extraction rate, use of organic solvents, large amount of waste water and low product purity in the production of chlorogenic acid. question

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 10kg of dried eucommia leaves, crush them and pass through a 80-mesh sieve, add 120kg of water, adjust the temperature to 50°C, keep the temperature constant and adjust the pH value to 4, the amount of cellulase added is 6000U / kg of material, and enzymatically hydrolyze for 2 hours under this condition ; After the enzymolysis is completed, add acid protease in an amount of 20,000 U / kg material, adjust the pH to 4.0, and enzymatically hydrolyze at 45°C for 2 hours; The extraction rate of chlorogenic acid is 84.2%. The operating conditions are P=1.0Mpa, T=45℃, pH=4.4, Q=70L / min. The filtrate is passed through a 10000Dal ultrafiltration membrane, and the extract is concentrated by 11.2 times under these conditions. The average flux of the ultrafiltration membrane is 236.3L / m 2 h; under operating conditions P=1.0Mpa, T=50°C, pH=4.4, Q=20 L / min, pass the ultrafiltration filtrate through a 150~300Dal nanofiltration membrane, and the ultrafiltration filtrate is concentrat...

Embodiment 2

[0033] Take 10kg of dried Eucommia leaves, crush them and pass through an 80-mesh sieve, add 120Kg of water, raise the temperature to 58°C, adjust the pH to 5.5, add cellulase, the addition amount is 10,000 U / kg of material, and enzymatically hydrolyze for 2.5h; After completion, add acid protease at an amount of 300,000 U / kg of material, raise the temperature to 60°C, adjust the pH to 4.5, and perform enzymatic hydrolysis for 2.5 hours. The extraction rate of original acid is 93.1%. The operating conditions are P=1.3Mpa, T=30℃, pH=4.8, Q=60L / min. The filtrate passes through a 10000Dal ultrafiltration membrane, and the extract is concentrated 12.8 times under this condition. The average flux of the ultrafiltration membrane is 228.3L / m 2 h; under the operating conditions P=1.3Mpa, T=50℃, pH=4.5, Q=20 L / min, the ultrafiltration filtrate is then concentrated through a 150~300Dal nanofiltration membrane, and the ultrafiltration membrane filtrate is concentrated under this conditio...

Embodiment 3

[0035] Take 10kg of dried Eucommia leaves, crush them and pass through a 80-mesh sieve, add 120Kg of water, raise the temperature to 60°C, adjust the pH to 6.0, add cellulase, the addition amount is 12000U / kg material, and enzymolyze for 4 hours; after the enzymolysis is completed, add Acid protease, the addition amount is 500,000 U / kg material, the temperature is raised to 65°C, the pH is adjusted to 5, the enzymolysis is 3h, after the enzymolysis is completed, the temperature is raised to 80~85°C to inactivate the enzyme treatment for 35min, conventional filtration, the extraction rate of chlorogenic acid is 93.8%; the operating conditions are P=1.3Mpa, T=50℃, pH=4.8, Q=70L / min. The filtrate passes through a 10000Dal ultrafiltration membrane, and the extract is concentrated by 13.2 times under this condition. The flux is 216.1L / m 2 h; under the operating conditions P=1.3Mpa, T=30℃, pH=4.8, Q=30 L / min, the ultrafiltration filtrate is then concentrated through a 300Dal nanofil...

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Abstract

The invention discloses a method for preparing chlorogenic acid from eucommia leaves. The chlorogenic acid in the eucommia leaves is extracted from warm water by adding a small amount of cellulase and protease; and the extracted chlorogenic acid is separated and purified by combination of a two-stage membrane separation technology and a resin adsorption-elution technology. The method effectively solves the problems such as low extraction ratio, adoption of organic solvent, large quantity of waste water and low product purity in the former production of the chlorogenic acid; and the content of the chlorogenic acid in the powder prepared by the technical method is higher than 55% and the yield of the final products is higher than 65%.

Description

technical field [0001] The invention relates to a method for preparing chlorogenic acid by using eucommia leaves, in particular to a method for preparing chlorogenic acid by using eucommia leaves by using enzymatic warm water extraction, membrane separation technology combined with resin technology. Background technique [0002] Chlorogenic acid is an important biologically active substance and chemical reagent. In my country, 170 kinds of Chinese patent medicines with antibacterial and anti-inflammatory effects all contain chlorogenic acid. It has a variety of pharmacological effects such as choleretic, antibacterial, antiviral, antihypertensive, white blood cell increase, and central nervous system stimulation. It is an important raw material for industries such as health care products, food, medicine, and cosmetics. Chlorogenic acid is mainly found in natural plants such as Eucommia ulmoides, honeysuckle, and coffee beans. Eucommia ulmoides Oliv (Eucommia ulmoides Oliv)...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/732C07C67/56
Inventor 万端极曾宪纲李丹刘冬成李晨悦欧阳道福胡瑞连
Owner PERFECT CHINA
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