Method for preparing total flavonoids in eucommia ulmoides leaves

Abstract

The present invention discloses a method for preparing total flavonoids in eucommia ulmoides leaves. The method comprises steps of eucommia ulmoides leaf crushing, cellulase assisted medium-temperature water extraction, conventional filtration, impurity removing with ultra-filtration membrane, nano-filtration concentration, separation and purification with macroporous resin, concentration and drying to prepare the total flavonoid powder. According to the present invention, the method adopts the cellulase assisted medium-temperature water extraction to extract the total flavonoids in the eucommia ulmoides leaves, wherein the extraction rate can achieve 95%, the water extraction temperature is low, and the extraction time is shortened; the ultra-filtration membrane is adopted to remove impurities from the extraction solution and separate other byproducts, wherein the membrane process is simple, the membrane flux can be stable for a long time, and characteristics of easy cleaning, long service life and easy industrialization achievement are provided; the nano-filtration membrane is adopted to concentrate the impurity-removed extraction solution so as to reduce the load of the follow-up process and prolong the service life of the resin; the COD value of the nano-filtration filtrate is less than or equal to 100, such that the nano-filtration filtrate can be reused for the production process, and almost no wastewater is discharged; and the macroporous resin is adopted so as to effectively purify the total flavonoids, wherein the purity of the total flavonoids in the prepared powder is more than 70%, and the extraction rate is more than 4%.

Description

technical field

[0001] The invention relates to a method for preparing total flavonoids from leaves of Eucommia ulmoides, in particular to a method for preparing total flavonoids by adopting an enzymatic medium-warm water extraction, membrane separation technology and resin technology. Background technique

[0002] Total flavonoids are an important biologically active substance, which has various pharmacological effects such as protecting the liver and gallbladder, antihypertensive, protecting nerve cells, anti-inflammation, anti-tumor, anti-thrombosis, and inhibiting osteoporosis. Wide range of applications. Total flavonoids mainly exist in natural plants such as eucommia and gardenia. Eucommia ulmoides is a unique economic tree species in my country, with a large distribution area and rich resources. The content of total flavonoids in Eucommia leaves is 2 to 5%, which is an ideal raw material for extracting total flavonoids. The extraction rate is not high in the existing...

Examples

Embodiment Construction

[0024] Example 1, see the attached figure 1 , take 10Kg of dried Eucommia leaves, crush them and pass through a 80-mesh sieve, add 120Kg of water, adjust the temperature to 55°C, keep the temperature constant and adjust the pH value to 4.5, then add 20g of cellulase, extract for 60min under this condition, filter, and filter the residue again Extract for the second time according to the aforementioned extraction conditions, combine the two filtrates, and filter conventionally. 0.03μm ultrafiltration membrane, the extract is concentrated 12.1 times under this condition, and the average flux of the ultrafiltration membrane is 222.0L / m 2 h; under operating conditions P=1.5Mpa, T=50°C, PH=4.5, Q=20 L / min, pass the ultrafiltration filtrate through a 200 molecular weight nanofiltration membrane, and the ultrafiltration filtrate is concentrated 17.0 times under this condition, The removal rate of inorganic salt ions is 62%, and the average flux of the nanofiltration membrane is 18....

technical field

[0001] The invention relates to a method for preparing total flavonoids from leaves of Eucommia ulmoides, in particular to a method for preparing total flavonoids by adopting an enzymatic medium-warm water extraction, membrane separation technology and resin technology. Background technique

[0002] Total flavonoids are an important biologically active substance, which has various pharmacological effects such as protecting the liver and gallbladder, antihypertensive, protecting nerve cells, anti-inflammation, anti-tumor, anti-thrombosis, and inhibiting osteoporosis. Wide range of applications. Total flavonoids mainly exist in natural plants such as eucommia and gardenia. Eucommia ulmoides is a unique economic tree species in my country, with a large distribution area and rich resources. The content of total flavonoids in Eucommia leaves is 2 to 5%, which is an ideal raw material for extracting total flavonoids. The extraction rate is not high in the existing...

Examples

Embodiment Construction

[0024] Example 1, see the attached figure 1 , take 10Kg of dried Eucommia leaves, crush them and pass through a 80-mesh sieve, add 120Kg of water, adjust the temperature to 55°C, keep the temperature constant and adjust the pH value to 4.5, then add 20g of cellulase, extract for 60min under this condition, filter, and filter the residue again Extract for the second time according to the aforementioned extraction conditions, combine the two filtrates, and filter conventionally. 0.03μm ultrafiltration membrane, the extract is concentrated 12.1 times under this condition, and the average flux of the ultrafiltration membrane is 222.0L / m 2 h; under operating conditions P=1.5Mpa, T=50°C, PH=4.5, Q=20 L / min, pass the ultrafiltration filtrate through a 200 molecular weight nanofiltration membrane, and the ultrafiltration filtrate is concentrated 17.0 times under this condition, The removal rate of inorganic salt ions is 62%, and the average flux of the nanofiltration membrane is 18....

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