The invention relates to an enteromorpha polysaccharide oral liquid and a preparation method thereof. The invention is characterized by adopting the following technical scheme: 1, obtaining enteromorpha polysaccharide by using enteromorpha as a raw material through purification, drying, pulverizing, extracting with hot water, enzymolysis, enzyme deactivation, centrifuging purification, ethanol precipitation and vacuum drying in sequence; 2, weighing 30-40% of lentinula edodes, 10-30% of kelp, 10-30% of yams and 10-30% of Chinese wolfberries in percentage by weight, and preparing a Chinese medicinal herb extract for later use through procedures of mixing, pulverizing, extracting in hot water, centrifuging, concentration, vacuum drying and the like; and 3, mixing 5-15% of enteromorpha polysaccharide, 5-15% of Chinese medicinal herb extract, 5-10% of sweetening agents and the balance of water in percentage by weight, and sterilizing, bottling, sealing, inspecting and packing the mixed liquid to obtain a finished product. The enteromorpha polysaccharide oral liquid is rich in nutrition and high in safety, and has multiple health-care functions of reducing blood sugar, reducing blood fat, enhancing the immunity, resisting tumors and the like.