Method for extracting and separating chlorogenic acid from honeysuckle

A technology of honeysuckle and chlorogenic acid, which is applied in the field of medicine, can solve the problems of lack of large-scale preparation, etc., and achieve the effect of easy industrial production, simple method and few production links

Active Publication Date: 2010-09-22
SHANGHAI INOHERB COSMETIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, there is a lack of suitable for large-scale preparation in the current prior art, which can purify the chlorogenic acid in honeysuckle to more than 98.0% method

Method used

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  • Method for extracting and separating chlorogenic acid from honeysuckle
  • Method for extracting and separating chlorogenic acid from honeysuckle
  • Method for extracting and separating chlorogenic acid from honeysuckle

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Weigh 1Kg of honeysuckle medicinal material, heat and reflux with 30% ethanol to extract three times, each time for 1 hour, filter, concentrate the extract until it has no alcohol smell, and continue to concentrate until the amount of extract contained in 1 ml is approximately equivalent to 0.5g of crude drug , add 10 volumes of water, refrigerate for more than 12 hours, filter, and concentrate the filtrate under reduced pressure until the amount of extract contained in 1 ml is approximately equivalent to 0.5 g of crude drug, add ether for degreasing three times, 1000 ml each time, take the aqueous solution layer, Pass through the D101 macroporous resin at a flow rate of 1 times the column volume, wash with water for 4 times the column volume after completion, collect the effluent and water washings, concentrate until the amount of extract contained in 1 ml is approximately equivalent to 1g of crude drug, cool to room temperature, 10 % sulfuric acid to adjust the pH valu...

Embodiment 2

[0028] Weigh 1Kg of honeysuckle medicinal material, heat and reflux with 40% ethanol to extract three times, each time for 1 hour, filter, concentrate the extract until it has no alcohol smell, continue to concentrate until the amount of extract contained in 1 ml is approximately equivalent to 0.5g crude drug , add 10 volumes of water, refrigerate for more than 12 hours, filter, and concentrate the filtrate under reduced pressure until the amount of extract contained in 1 ml is approximately equivalent to 0.5 g of crude drug, add petroleum ether for degreasing three times, 1000 ml each time, and take the aqueous solution layer , pass through the D101 macroporous resin at a flow rate of 1 times the column volume, wash with water for 4 times the column volume after completion, collect the effluent and water washings, concentrate until the amount of extract contained in 1 ml is approximately equivalent to 1g of crude drug, cool to room temperature, Adjust the pH value to 1.8 with ...

Embodiment 3

[0030] Weigh 1Kg of honeysuckle medicinal material, heat and reflux with 50% methanol to extract three times, each time for 1 hour, filter, concentrate the extract until it has no alcohol smell, and continue to concentrate until the amount of extract contained in 1 ml is equivalent to about 0.5g of crude drug , add 10 volumes of water, refrigerate for more than 12 hours, filter, and concentrate the filtrate under reduced pressure until the amount of extract contained in 1 ml is approximately equivalent to 0.5 g of crude drug, add petroleum ether for degreasing three times, 1000 ml each time, and take the aqueous solution layer , pass through the D101 macroporous resin at a flow rate of 1 times the column volume, wash with water for 4 times the column volume after completion, collect the effluent and water washings, concentrate until the amount of extract contained in 1 ml is approximately equivalent to 1g of crude drug, cool to room temperature, Adjust the pH value to 1.8 with ...

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Abstract

The invention relates to the field of the medicine, in particular to a method for extracting and separating a chlorogenic acid monomer from honeysuckle. The prior art is lack of a method for the mass preparation of the chlorogenic acid monomer from the honeysuckle. In the invention, the technology is realized by the following preparation steps of: (1) soaking by alcohol: putting the honeysuckle into an alcohol solution for soaking, heating, refluxing, filtering and concentrating; (2) degreasing: degreasing the obtained filtrate thrice by adopting a weak-polarity solvent, and taking a water layer; (3) enriching by macroporous resin: enabling a water layer solution obtained in the step (2) to pass through the macroporous resin, washing by water, collecting effluent liquid and water washing liquid, concentrating and regulating the pH value; (4) extracting: extracting a solution obtained in the step (3) by adopting a moderate-polarity solvent, taking an organic layer, concentrating and recovering the solvent to obtain a faint yellow puffy solid substance; and (5) recrystallizing. The invention adopts an alcohol extraction method to enhance the extraction rate and the transfer rate of the chlorogenic acid, the final transfer rate of the chlorogenic acid is as high as 84 percent, and the purity of the obtained product can reach more than 98 percent.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a method for extracting and separating chlorogenic acid monomers from honeysuckle. The content of the chlorogenic acid monomer separated by the method reaches more than 98.0%. Background technique [0002] Honeysuckle, also known as two flowers, double flowers, golden flowers, silver flowers and honeysuckle flowers, belongs to the genus Lonicera in the Lonicera family. According to literature records: my country is one of the origins of honeysuckle, which was called honeysuckle in the "Shen Nong's Materia Medica" in the Qin and Han Dynasties more than 2,200 years ago. Li Shizhen of the Ming Dynasty gave a detailed introduction to the name, shape and characteristics of honeysuckle in his "Compendium of Materia Medica". It has a sweet taste and a cold nature. With the research of modern chemistry and pharmacology, its main active ingredients have been basically clear, but one of its main ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48C07C67/56
Inventor 张彤谢景田陶建生蔡贞贞丁越
Owner SHANGHAI INOHERB COSMETIC
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