MALDI-TOF MS (Matrix-assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry)-assisted identification method for listeria monocytogenes

A MALDI-TOF-MS, Listeria technology, applied in the field of detection of Listeria monocytogenes, to achieve the effect of simple sample preparation and accurate identification
CN102253111AInactive Publication Date: 2011-11-23曹际娟 +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
曹际娟
Publication Date
2011-11-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

This invention relates to an MALDI-TOF MS (Matrix-assisted Laser Desorption / Ionization Time of Flight Mass Spectrometry)-assisted identification method for listeria monocytogenes, comprising the following steps of: (1) selecting 35 to 40 strains of fresh cultures of listeria monocytogenes, pre-treating the samples; (2) collecting maps of all bacterial strain samples; (3) analyzing and unifying the obtained maps by using BioTyper software to obtain a standard map of listeria monocytogenes; (4) preparing a sample of a microorganism to be detected by adopting the method in the step (1); (5) collecting the MALDI-TOF-MS map of the sample to be detected according to the method in the step (2); comparing the obtained MALDI-TOF-MS map of the sample to be detected in step (5) with the obtained standard map of listeria monocytogenes in the step (3), judging the detection results according to matching fractions. According to the invention, a mass spectrogram database and the standard map in listeria monocytogenes identification are created successfully, and the accurate identification with convenience, microscale and high automation for listeria monocytogenes can be realized.
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Description

technical field

[0001] The invention relates to a new detection method for Listeria monocytogenes, in particular to a method for assisting identification by using a matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF MS). Background technique

[0002] Listeria monocytogenes is a zoonotic pathogen. The bacteria can cause meningitis, encephalitis, sepsis, miscarriage, stillbirth and other diseases in pregnant women and animals. Pregnant women, newborns, the elderly and immunocompromised persons are susceptible groups. Although the incidence of foodborne Listeria is not high, the mortality rate can sometimes reach 20% to 50%. WHO listed it as one of the four major food pathogens in the 1990s. The fungus is widely distributed in nature, with livestock and poultry as the main host, and it is easy to contaminate food and cause food poisoning and listeria outbreaks [4] . Meat, eggs, poultry, seafood, dairy products, vegetables, etc. have all...

Claims

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