Xylose fermentation strain resistant to inhibitors and construction method and applications thereof

Abstract

The invention discloses a xylose fermentation strain resistant to inhibitors. The strain is named as SEB11 and has a preservation number of CGMCC No. 16570. The invention also relates to a construction method of the above-mentioned xylose fermentation strain, and the method comprises the steps of: selecting a starting strain SEB5; performing plasma mutagenesis treatment on the starting strain, andpicking out bacterial colonies which are better or similar to the starting strain in growth situation; and subjecting the picked strains to plate initial screening and actual saccharification liquidrescreening so as to obtain the xylose fermentation strain SEB11 resistant to inhibitors. The present invention also relates to applications of the above xylose fermentation strain in producing ethanol by using xylose in lignocellulose. The method adopts an industrial strain of the xylose as a starting strain, adopts a normal-pressure room-temperature plasma mutagenesis technique for mutagenesis,and obtains the xylose fermentation mutant strain SEB11 with excellent inhibitor tolerance according to the growth condition in a plate medium containing inhibitors and the actual saccharification liquid fermentation rescreening. The xylose consumption rate is significantly increased, and a better fermentation result exhibits.

Description

technical field

[0001] The invention relates to a yeast, in particular to a xylose fermentation strain resistant to inhibitors and its construction method and application. Background technique

[0002] With the depletion of fossil resources such as petroleum and the intensification of the greenhouse effect, it has become the focus of people's attention to find alternatives to petroleum resources. Using lignocellulose to produce fuel ethanol to replace fossil fuels can effectively solve the energy crisis and environmental pollution caused by fossil fuels. However, several obstacles exist in the conversion of lignocellulosic ethanol, including inhibitor tolerance and low xylose utilization. Inhibitors formed by acid-catalyzed hydrolysis of lignocellulose (mainly including furan derivatives, weak acids, and phenolic compounds) reduce the growth rate and fermentation of S. cerevisiae, thereby reducing ethanol production. Although there are many reports on the ability of Saccha...

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