Metabolic Engineering yeast using xylose fermentation for producing ethanol

Abstract

Xylose fermentation ethanol is one of the key technologies utilizing biomass to produce ethanol. A saccharomyces cerevisiae engineering bacterial strain is set up by a method of metabolic engineering. The bacterial strain not only can ferment the ethanol produced by the xylose, but also can automatically balance oxido-reduction cofactor, so as to be a metabolic engineering microzyme which has little by-product and can effectively use xylose fermentation for producing the ethanol. The conversion rate of xylose fermentation ethanol of bacterial strain reaches 88%, thus laying the foundation for utilizing the biomass to produce fuel ethanol.

Description

technical field

[0001] The invention belongs to the field of biotechnology, in particular to a Saccharomyces cerevisiae engineering strain E33 which efficiently utilizes xylose to ferment ethanol and its application. Background technique

[0002] With the depletion of petroleum resources, ethanol has replaced part of gasoline as a clean fuel, and the demand is increasing year by year. At present, the raw material for ethanol production is mainly starch from grain, and the cost of raw material accounts for more than half of the entire production cost, which has become a bottleneck for the large-scale promotion of fuel ethanol. Biomass is the most abundant renewable resource on earth. The use of biomass to produce fuel ethanol is a hot spot in international competition. After the carbohydrates in biomass are completely degraded into simple sugars, nearly one-third is xylose. Efficient conversion of xylose to produce ethanol is not only environmentally friendly but also condu...

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