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Method for creating distiller's yeast ethanol high yield bacterial strain

A technology of Saccharomyces cerevisiae and high-yield strains, which is applied in the field of genome-wide transformation, can solve the problems that eukaryotes have not been applied, and the method of protoplast fusion cannot be directly applied, and achieve the effect of shortening the fermentation cycle

Inactive Publication Date: 2008-12-17
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although genome rearrangement technology has been successfully applied to prokaryotes, the complexity of eukaryotes prevents genome rearrangement technology from being applied to yeast research.
For example, yeast has a thicker cell wall (100-200nm), and the fusion of yeast is a complex process controlled by the MAT allele, etc. These factors make it impossible to directly apply the method of protoplast fusion in yeast

Method used

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  • Method for creating distiller's yeast ethanol high yield bacterial strain
  • Method for creating distiller's yeast ethanol high yield bacterial strain
  • Method for creating distiller's yeast ethanol high yield bacterial strain

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Embodiment 1

[0020] A method for constructing a high-yield ethanol-producing strain of Saccharomyces cerevisiae, using the laboratory Saccharomyces cerevisiae diploid strain W303 (preserved in this laboratory) as the starting strain to construct a heterogeneous strain with excellent fermentation properties such as high ethanol production, stable genetics, and short fermentation cycle. Ploid yeast strains.

Embodiment 2

[0022] A method for constructing a high-yielding strain of Saccharomyces cerevisiae, using industrial Saccharomyces cerevisiae diploid strains (purchased from Angel Yeast Co., Ltd.) Aneuploid yeast strain with high yield and low residual sugar.

[0023] Proceed as follows:

[0024] 1 Materials and methods

[0025] 1.1 Materials

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Abstract

The invention discloses a method for constructing the ethanol high-yield strains of Saccharomyces cerevisiae, which comprises the following steps: (1) ethylmethane sulfonate is utilized to carry out random mutagenesis to the amphiploid strains of the Saccharomyces cerevisiae to cause the final concentration of the volume of the ethylmethane sulfonate in the bacteria liquid to be processed to be 2 to 4 percent and the concentration of the amphiploid strains of the Saccharomyces cerevisiae to be 2 multiplied by 10<6> to 6 multiplied by 10<6> cell / ml; (2) 3 to 5 rounds of the sexual recombination of high efficient sporulation, spore purification and full integration cause genome recombination between strains in a library to construct the ethanol high-yield strains of the Saccharomyces cerevisiae. The ethanol output of the recombinant strains constructed by the method of the invention is increased by 8.88 percent compared with that of contrast strains, residual sugar is reduced by 64.37 percent, the fermentation cycle is shortened by 10 hours and ethanol and high osmotic resistance are 5.10 times of that of the contrast strains, thus providing excellent strains for the industrial production of fuel ethanol.

Description

technical field [0001] The invention relates to a whole genome transformation method, in particular to a method for constructing a high-yield ethanol-producing strain of Saccharomyces cerevisiae. Background technique [0002] High-concentration ethanol is the main factor for the slow or even termination of yeast ethanol fermentation in the late stage of fermentation and the high residual sugar at the end of fermentation. Screening yeast strains resistant to high-concentration ethanol is one of the important strategies to improve the ethanol fermentation performance of Saccharomyces cerevisiae. Meanwhile, most fermentation-related traits of yeast are controlled by polygenes, which are poorly understood and widely distributed throughout the genome. At present, although genome rearrangement technology has been successfully applied to prokaryotes, the complexity of eukaryotes prevents genome rearrangement technology from being applied to yeast research. For example, yeast has a...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N15/01C12P7/06C12R1/865
CPCY02E50/10
Inventor 侯丽华马平生
Owner TIANJIN UNIV