Glycerol channel protein gene deleted brewing microzyme strain capable of reducing glycerol output and increasing ethanol output and construction method thereof

A technology of Saccharomyces cerevisiae strain and channel protein, which is applied in the field of Saccharomyces cerevisiae biological fermentation, can solve the problems of long fermentation period, environmental pollution, and high fermentation by-products, reduce the production of glycerol by-products, solve high production costs, and increase ethanol production Effect
CN1763175AActive Publication Date: 2006-04-26TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2006-04-26

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Abstract

The present invention discloses one kind of Saccharomyces cerevisiae strain with glycerin passage protein gene deficiency and resulting in lowered glycerin generation and raised ethanol yield and its construction process. The construction process includes the following steps: 1. obtaining Saccharomyces cerevisiae monoploid and deleting of URA3 gene; 2. constructing PUC18-FPS1-ORF plasmid; 3. constructing PUC18-FUF plasmid; and 4. constructing KAM-21 strain. The present invention deletes glycerin passage protein FPS1 gene code embedded on cell membrane and loses the expression of FPS1 gene by means of gene engineering technology, so as to inhibit glycerin synthesizing way, convert from glycerin metabolism to ethanol metabolism, increase ethanol yield, and solve serial problems in ethanol production.
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Description

Technical field

[0001] The invention belongs to the field of Saccharomyces cerevisiae biological fermentation, specifically the ethanol fermentation industry. In particular, it relates to a strain of Saccharomyces cerevisiae modified by genetic engineering and a method for constructing the strain. Background technique

[0002] Ethanol has been a renewable energy source that partially or completely replaces petroleum, and is of great significance to the sustainable development of human society. However, the main problems in the production of ethanol by industrial fermentation at home and abroad are as follows: high fermentation by-products, long fermentation cycle, low ethanol conversion rate and serious environmental pollution. Glycerol is the main by-product in the ethanol fermentation process, which consumes about 10% of fermentable sugars. The production of glycerol by-products directly affects the sugar-alcohol conversion rate. It has become a major obstacle to reducing the c...

Claims

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