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Method for preparing aerobiotic nitrifier immobilizing vector

A technology of immobilized carriers and nitrifying bacteria, applied in the direction of immobilized on or in inorganic carriers, fixed on/in organic carriers, chemical instruments and methods, etc., can solve the problem of poor stability of immobilized carriers and poor comprehensive performance Improvement, short service life and other problems, to achieve the effect of high reuse rate, large specific surface area, and improved removal rate

Inactive Publication Date: 2013-04-03
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the immobilized microorganisms prepared by the embedding method have a certain impact on mass transfer, and the stability of the immobilized carrier is poor, and the service life is short. Therefore, the comprehensive performance of the immobilized microbial carrier needs to be improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The attapulgite ore produced in Xuyi was pulverized by a rapid crusher, passed through a 200-mesh sieve, and 5g of attapulgite powder was placed in 50ml of hydrochloric acid solution (concentration 1mol / L), soaked at 70°C for 2h, and then used Distilled water was filtered and washed until neutral, then calcined in a muffle furnace at 200°C for 2 hours, cooled, and ground for later use;

[0020] The high-efficiency activated sludge taken from the biochemical tank of the sewage treatment plant is added to the nitrifying bacteria enrichment culture solution. Under the conditions of 28-30 ° C and pH 7.0-8.0, intermittent aeration is adopted, and the water is changed once every two days. Clarify the water before removing the supernatant, add an equal volume of nitrifying bacteria enrichment culture solution, enrich the culture for 28 days, and set aside; the nitrifying bacteria enrichment culture solution is glucose 14.1g / L, NH 4 CI 1.34g / L, KH 2 PO 4 0.7g / L, MgSO...

Embodiment 2

[0023] After the attapulgite ore produced in Xuyi was pulverized by a quick pulverizer, passed through a 200-mesh sieve, 5g of attapulgite powder was placed in 50ml of hydrochloric acid solution (concentration 6mol / L), soaked at 70°C for 4h, and then used Distilled water was filtered and washed until neutral, then calcined in a muffle furnace at 250°C for 2 hours, cooled, and ground for later use;

[0024] The high-efficiency activated sludge taken from the biochemical tank of the sewage treatment plant is added to the nitrifying bacteria enrichment culture solution. Under the conditions of 28-30 ° C and pH 7.0-8.0, intermittent aeration is adopted, and the water is changed once every two days. Clarify the water before removing the supernatant, add an equal volume of nitrifying bacteria enrichment culture solution, enrich the culture for 28 days, and set aside; the nitrifying bacteria enrichment culture solution is glucose 14.1g / L, NH 4 CI 1.34g / L, KH 2 PO 4 0.7g / L, MgSO 4...

Embodiment 3

[0027] The attapulgite ore produced in Xuyi was pulverized by a rapid crusher, passed through a 200-mesh sieve, and 5g of attapulgite powder was placed in 50ml of hydrochloric acid solution (concentration 2mol / L), soaked at 70°C for 4h, and then used Distilled water was filtered and washed until neutral, then calcined in a muffle furnace at 250°C for 2 hours, cooled, and ground for later use;

[0028] The high-efficiency activated sludge taken from the biochemical tank of the sewage treatment plant is added to the nitrifying bacteria enrichment culture solution. Under the conditions of 28-30°C and pH 7.0-8.0, intermittent aeration is adopted, and the water is changed once every two days. Clarify the water before removing the supernatant, add an equal volume of nitrifying bacteria enrichment culture solution, enrich the culture for 28 days, and set aside; the nitrifying bacteria enrichment culture solution is glucose 14.1g / L, NH 4 CI 1.34g / L, KH 2 PO 4 0.7g / L, MgSO 4 0.5g / ...

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Abstract

The invention discloses a method for preparing an aerobiotic nitrifier immobilizing vector, and belongs to the field of water treatment. The method comprises the steps of: firstly, carrying out acid washing on thermally modified attapulgite; adsorbing the nitrifier using the nanometer grade attapulgite; carrying out rapid inclusion by using the mixture gel of polyvinyl alcohol (PVA) and sodium alginate (SA); dropping the mixture into a crosslinking agent to soak for 24 hours using a granulator to obtain an immobilized nitrifier pellet; and finally, repeatedly washing the immobilized nitrifier pellet for three times using normal saline to obtain a vector particle. By taking advantage of pretty large specific surface area and pretty strong adsorption performance of the modified attapulgite, an adsorption vector which is conductive to the growth and reproduction of the aerobiotic nitrifier is prepared; the modified attapulgite is capable of ammonia and nitrogen adsorption, and can increase the ammonia and nitrogen removal rate in water; the carrier particle composited from the modified attapulgite and a macromolecular material can improve the activity and ammonia and nitrogen removal capacity of denitrification microorganisms; and the repeated use rate of the carrier is high.

Description

technical field [0001] The invention belongs to the field of water treatment, and in particular relates to a method for preparing an aerobic nitrifying bacteria immobilized carrier. Background technique [0002] With the acceleration of urbanization in my country and the improvement of people's living standards, the requirements for the surrounding urban environment are getting higher and higher. As an important carrier of resources and environment in urban development, urban inland rivers are related to the healthy development of cities and restrict them. Urban development is an important factor affecting urban style and beautifying the urban environment. At present, the sources of water pollution are not under control, the direct discharge of industrial wastewater and domestic sewage is serious, the water in urban rivers turns black and smelly, and the phenomenon of algae reproduction is becoming more and more serious. According to surveys, about 50% of urban rivers in my ...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C12N11/08C02F3/34C02F101/16
Inventor 梁玉婷宋超鹏杜尔登
Owner CHANGZHOU UNIV
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