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Device for simulation of digestion in small intestine and use method thereof

A technology for the small intestine and digestive products, which is applied in the direction of measuring devices, testing food, instruments, etc., and can solve the problems of insufficient detail and little correlation of the control of the small intestine simulation system

Inactive Publication Date: 2014-06-04
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, these patents are not detailed enough to control the parameters in the small intestine simulation system. Some of these patents are only based on animal-related parameters and have little relevance to humans.

Method used

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  • Device for simulation of digestion in small intestine and use method thereof
  • Device for simulation of digestion in small intestine and use method thereof
  • Device for simulation of digestion in small intestine and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Intestinal simulated digestion of plant polysaccharides.

[0016] (1) Preparation of small intestinal juice.

[0017] Intestinal juice was obtained by weighing 100 g intestinal electrolyte solution (intestinal electrolyte solution preparation: weigh 5 g NaCl, 0.5 g KCl, 0.3 g CaCl 2 , was dissolved in a 1 L volumetric flask with deionized water, and the pH of the solution was adjusted to 7 with 0.1 M NaOH), 100 g (7.5%, w / w) trypsin solution, 14 mg trypsin, 220 g water . The pH of the mixed solution was adjusted to 7 with 0.2 M NaOH.

[0018] (2) Preparation of bile.

[0019] Bile is a 2% solution prepared by dissolving pig gall powder in deionized water.

[0020] (3) Simulate small intestine digestion.

[0021] Firstly, the plant polysaccharide solution (5 mg / ml) that has been simulated digestion in the oral cavity and stomach in advance is placed in the sample holding dish 1 .

[0022] Then, small intestinal digestion was grouped as follows using a controlled t...

Embodiment 2

[0032] Small intestine simulated digestion of cornstarch.

[0033] (1) Preparation of small intestinal juice.

[0034] Intestinal juice was obtained by weighing 100 g intestinal electrolyte solution (intestinal electrolyte solution preparation: weigh 5.4 g NaCl, 0.65 g KCl, 0.33 g CaCl 2 , dissolved in 1 L volumetric flask with deionized water, adjusted the pH of the solution to 7.5 with 0.1 M NaOH), 100 g (7%, w / w) trypsin solution, 13 mg trypsin, 200 g water . The pH of the mixed solution was adjusted to 7.5 with 0.1 M NaOH.

[0035] (2) Preparation of bile.

[0036] Bile is prepared by dissolving pig gall powder in deionized water to form a 4% solution.

[0037] (3) Simulate small intestine digestion.

[0038] First, the cornstarch solution (5 mg / ml) that has been simulated digestion in the oral cavity and stomach in advance was placed in the sample holding dish 1 .

[0039] Then, small intestinal digestion was grouped as follows using a controlled test method.

...

Embodiment 3

[0049] (1) Preparation of small intestinal juice.

[0050] Intestinal juice was obtained by weighing 100 g intestinal electrolyte solution (intestinal electrolyte solution preparation: weigh 5 g NaCl, 0.6 g KCl, 0.3 g CaCl 2 , dissolved in a 1 L volumetric flask with deionized water, adjusted to pH 7.5 with 0.1 M NaOH), 100 g (7%, w / w) trypsin solution, 14 mg trypsin, 200 g water . The pH of the mixed solution was adjusted to 7.5 with 0.2 M NaOH.

[0051] (2) Preparation of bile.

[0052] Bile is a 3% solution prepared by dissolving pig gall powder in deionized water.

[0053] (3) Simulate small intestine digestion.

[0054] First, the soy protein solution (5 mg / ml) that has been simulated digestion in the oral cavity and stomach in advance is placed in the sample holding dish 1 .

[0055] Then, small intestinal digestion was grouped as follows using a controlled test method.

[0056] Experimental group 1: add small intestinal juice, bile and soybean protein solution...

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Abstract

The invention discloses a device for simulation of digestion in small intestine and a use method thereof. The device comprises a small intestine reactor, a stirrer, a water-bath control device, a computer monitoring system and a small intestine evacuation pump. A small intestinal fluid is added into the small intestine reactor, a solution or a dispersion liquid to be detected is added into the small intestine reactor, a simulator simulates small intestine peristalsis, the water-bath control device controls a temperature in the small intestine reactor, the computer monitoring system monitors an internal temperature, a pH value and digestion time of the small intestine reactor, the small intestine evacuation pump simulates small intestine evacuation, the digested product is collected, pancreatin and trypsin activity is eliminated, the digestion simulation process is repeated three times, and a digestion case of the material to be detected is detected. The device realizes first simulation of specific and detailed digestion in small intestine, realizes observation of digestion of various active materials or nutrients in the human small intestine, carries out simulation in a simulation environment designed according to a human physiological environment, has high conformity to the small intestine digestion and has high accuracy in simulation environment control.

Description

technical field [0001] The invention relates to a device for simulating small intestine digestion and a method for using it. Background technique [0002] The world's authoritative journals stated that it is very important to study the effects of food or active substances in the human body and their mechanisms of action, because the elucidation of the mechanism of action in vivo can provide a research basis for maximizing the use of food or active substances in the human body. It can exert its physiological efficacy more effectively, and at the same time, it has guiding significance for avoiding its possible adverse side effects. The actual physiological effect of a food or active substance depends largely on what is the product of its digestion by the body. For the consideration of ethics in human body research, simulating human digestion in vitro has become the technical key to the research on the mechanism of action of food or active substances in vivo. Dynamic simulati...

Claims

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Application Information

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IPC IPC(8): G01N33/00G01N33/02
Inventor 谢明勇聂少平胡婕伦
Owner NANCHANG UNIV
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