Lactobacillus plantarum with inhibitory activity to alpha-glucosidase

A technology of glucosidase and Lactobacillus plantarum, applied in the biological field, can solve problems such as side effects and adverse reactions, and achieve the effects of short growth cycle, simple production method and high activity

Active Publication Date: 2017-12-22
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the α-glucosidase inhibitor hypoglycemic drugs that have been clinically used mainly include acarbose, v

Method used

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  • Lactobacillus plantarum with inhibitory activity to alpha-glucosidase
  • Lactobacillus plantarum with inhibitory activity to alpha-glucosidase
  • Lactobacillus plantarum with inhibitory activity to alpha-glucosidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0124] Described protective agent comprises 5% skimmed milk and 0.5% L-glutamic acid, and the preparation of described active freeze-dried bacterial powder comprises the following steps:

[0125] Step A, insert the strain into MRS broth medium with 4% inoculum amount for mass fermentation, cultivate at 36°C for 16 hours, and after microscopic examination confirms that there is no pollution, then put the cultured bacterial solution at 4°C 6000r / min, centrifuge for 10min, collect the bacteria;

[0126] Step B, mixing the protective agent and the bacteria in a volume ratio of 1:1, and freeze-drying to obtain the bacteria powder containing the Lactobacillus plantarum.

Embodiment 2

[0128] Described protective agent comprises the L-glutamic acid of 10% skimmed milk and 1.0%, and the preparation of described active freeze-dried bacterial powder comprises the following steps:

[0129] Step A, insert the strain into MRS broth medium with 4% inoculum amount for mass fermentation, cultivate at 37°C for 18 hours, and after microscopic examination confirms that there is no pollution, then put the cultured bacterial solution at 4°C 6000r / min, centrifuge for 10min, collect the bacteria;

[0130] Step B, mixing the protective agent and the thallus at a volume ratio of 1:2, and freeze-drying to obtain the bacterium powder containing the Lactobacillus plantarum.

Embodiment 3

[0132] Described protective agent comprises the L-glutamic acid of 15% skimmed milk and 1.5%, and the preparation of described active freeze-dried bacterial powder comprises the following steps:

[0133] Step A, insert the strain into MRS broth medium with 4% inoculum amount for mass fermentation, cultivate at 38°C for 20 hours, and after microscopic examination confirms no pollution, then put the cultured bacterial solution at 4°C 6000r / min, centrifuge for 10min, collect the bacteria;

[0134] Step B, mixing the protective agent and the thallus at a volume ratio of 1:3, and freeze-drying to obtain the bacterium powder containing the Lactobacillus plantarum.

[0135] In the above scheme, the Lactobacillus plantarum in the active freeze-dried bacterial powder has a high inhibitory activity of α-glucosidase, and taking it within one hour after a meal can prevent the rapid rise of blood sugar in diabetic patients, and reduce the blood sugar level within 2 hours after a meal. The...

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Abstract

The invention discloses lactobacillus plantarum with the inhibitory activity to alpha-glucosidase. The lactobacillus plantarum is classified and named as Lactobacillus plantarum, is preserved on August 29, 2017 in China General Microbiological Culture Collection Center and has a preservation number of CGMCC NO.14573. By screening, the lactobacillus plantarum has in-vitro inhibitory activity to alpha-glucosidase; the survival rate of the lactobacillus plantarum reaches up to 71.04% under an acid condition that the pH value is 2.0 and is 77.14% in 2.0% cholate deionized water, the tolerances of the lactobacillus plantarum to artificially simulated saliva and intestinal fluid are higher than 90%, the tolerance of the lactobacillus plantarum to gastric fluid reaches 52.49%, and after artificially simulated fluid is sequentially digested, the survival rate reaches 88.27%; the lactobacillus plantarum has good effects on the prevention and treatment of diabetes mellitus type 2; the lactobacillus plantarum is added with a protection agent so as to prepare freeze-drying battier powder with a high viable count, and the viable count reaches up to 87% of the viable count before freeze-drying; and besides, the lactobacillus plantarum further has a good fermentation effect.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically, the invention relates to a strain of Lactobacillus plantarum with high alpha-glucosidase inhibitory activity and its screening and application. Background technique [0002] Diabetes mellitus is a metabolic disorder caused by insufficient insulin secretion (type 1) or insulin resistance (type 2) and is characterized by chronic hyperglycemia. At present, diabetes has become a major disease, which has caused great damage to human life and health. According to the data of the World Health Organization, by 2017, the prevalence of diabetes in China has ranked first in the world, with about 110 million people, accounting for about one-tenth of the total number of Chinese adults. According to the current trend, by 2040, there will be more than 150 million patients in my country, and the death rate from diabetes will be greater than the sum of the death rates from AIDS, tuberculosis and m...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/68C12Q1/34C12Q1/04A61K35/747A61P3/10A23L33/135C12R1/25
CPCA61K2035/115A23L33/135C12Q1/34C12Q1/689C12N1/205C12R2001/25
Inventor 刘鹭吕加平王芬张书文逄晓阳芦晶
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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