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Preparation method of duck simulated intestinal fluid

A technology for simulating small intestinal juice and small intestinal juice, which is applied in the field of animal simulated digestive juice preparation, can solve the problems of different sources of enzymatic properties and high implementation cost, and achieve the effects of low implementation cost, efficient production process, and reduced production cost

Inactive Publication Date: 2012-01-11
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation method of this simulated poultry small intestine juice uses a single digestive enzyme of reagent grade, and most of the digestive enzymes are pig-derived or microbial-derived, so there is a problem of different enzymatic properties, and the implementation cost is very high. It is difficult to be accepted by livestock enterprises for cost accounting of feed nutrient biological potency testing

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  • Preparation method of duck simulated intestinal fluid
  • Preparation method of duck simulated intestinal fluid

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preparation example Construction

[0018] Such as figure 1 As shown, the preparation method of duck simulated small intestine juice of the present invention comprises the following steps one to five:

[0019] Step 1: Put the duck small intestine chyme collected from the duck small intestine of the duck of the set amount of liquid preparation into a reagent bottle and shake well, and put the mixture obtained after shaking well into a centrifuge bottle (such as selecting 250 ml centrifuge bottle), at a set temperature (such as 4 ° C), centrifuge through a centrifuge, after the end of the centrifugation, collect the supernatant in the centrifuge bottle;

[0020] Step 2: After filtering the supernatant obtained in Step 1, freeze and condense it into a mixed concentrate by a freeze dryer;

[0021] Step 3: After the mixed concentrated solution obtained in Step 2 is dialyzed through a dialysis bag to remove impurities, then freeze-dried into freeze-dried powder;

[0022] Step 4: Measure the activity (activity per un...

Embodiment 1

[0035] Install poultry intestinal chyme collection sleeves in the small intestines of 20 8-week-old ducks with an average body weight of more than 3.0 kg. After 20 days of recovery from the operation, collect duck small intestines through the poultry intestinal chyme collection sleeves Digesta, 3 liters of duck small intestinal digesta was collected into a sample bottle kept at a low temperature of 4°C.

[0036] Put the collected 3 liters of duck small intestine digesta into a reagent bottle and shake well, put the shaken mixture into a 250ml centrifuge bottle, and centrifuge at 1250g for 10 minutes in a centrifuge at 4°C. Collect the supernatant in the centrifuge bottle after processing.

[0037] The obtained supernatant was filtered through a 200-mesh nylon filter cloth, and then lyophilized and concentrated for 12 hours, so that the supernatant was lyophilized and concentrated by 1.5 times to become the required mixed concentrate.

[0038] Transfer the obtained mixed conce...

Embodiment 2

[0043] A poultry intestinal chyme collection sleeve was installed in the small intestine of 30 12-week-old ducks with an average body weight of more than 3.2 kg. After 25 days of recovery from the operation, the duck small intestine was collected through the poultry intestinal chyme collection sleeve. Digesta, 5 liters of duck small intestine digesta was collected into a sample bottle at a low temperature of 8°C.

[0044] Put the collected 5 liters of duck small intestine digesta into a reagent bottle and shake well, put the shaken mixture into a 250ml centrifuge bottle, and perform 8 minutes of centrifugation at 1250g in a centrifuge at 4°C. Collect the supernatant in the centrifuge bottle after processing.

[0045] The obtained supernatant was filtered through a 200-mesh nylon filter cloth, and then freeze-concentrated by a freeze dryer for 36 hours, so that the supernatant was freeze-concentrated 10 times to become the required mixed concentrate.

[0046] Transfer the obta...

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Abstract

The invention discloses a preparation method of a duck simulated intestinal fluid. The method comprises the following steps of: putting collected duck intestinal chyme in a reagent bottle to shake uniformly, putting in a centrifuge bottle to perform centrifuging treatment, and collecting a supernatant fluid; filtering the supernatant fluid, freezing and concentrating into a mixed concentrated liquid; after dialyzing the mixed concentrated liquid and removing impurities, freezing and drying into lyophiled powder; measuring the activities of alpha-amylase, lipase, trypsin and chymotrypsin in the lyophiled powder, and measuring the activities of corresponding digestive enzymes in the reagent level alpha-amylase, lipase, trypsin and chymotrypsin; and according to the principal that the gross activities of various digestive enzymes in the duck simulated intestinal fluid and the targeted duck intestinal fluid are equal, adding corresponding capacity reagent level alpha-amylase, corresponding weight reagent level lipase, corresponding weight reagent level trypsin, corresponding weight reagent level chymotrypsin and a corresponding capacity water solution in the lyophiled powder with a set weight. The method can be carried out in a low-cost way, and can also be used for preparing the duck simulated intestinal fluid basically homologous with four digestive enzymes in a real duck body.

Description

technical field [0001] The invention relates to a preparation method of animal simulated digestive juice, in particular to a preparation method of duck simulated small intestine juice. Background technique [0002] my country's duck breeding accounts for more than 70% of the world's total. In the preparation of duck rations, the energy and amino acid biological potency of feed ingredients have always been the first parameters that need to be understood in feed formulation. However, it is time-consuming, labor-intensive and cost-intensive to determine the metabolizable energy and digestible amino acid data of feed ingredients by biological methods, which has become the main technical bottleneck for updating the basic data of feed ingredients in production. For a long time, nutritionists from various countries have tried to establish a technical system for rapid and quantitative determination of feed energy and amino acid biological potency by simulating the digestion of feed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/02
Inventor 赵峰张宏福侯水生张子仪
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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