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Program-controlled pig bionic digestion system and method using program-controlled pig bionic digestion system to quickly determining digestible energy value of pig feed

A digestive system, program-controlled technology, applied in the agricultural field, can solve problems such as rupture and inability to truly simulate large intestine digestion

Active Publication Date: 2019-07-26
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when this system simulates the digestion stage of the pig's large intestine, the cellulase that simulates the large intestine fluid can degrade the dialysis bag, causing it to rupture, which cannot truly simulate the digestion of the large intestine

Method used

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  • Program-controlled pig bionic digestion system and method using program-controlled pig bionic digestion system to quickly determining digestible energy value of pig feed
  • Program-controlled pig bionic digestion system and method using program-controlled pig bionic digestion system to quickly determining digestible energy value of pig feed
  • Program-controlled pig bionic digestion system and method using program-controlled pig bionic digestion system to quickly determining digestible energy value of pig feed

Examples

Experimental program
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Effect test

Embodiment 1

[0078] Such as Figure 1-2 Shown is a program-controlled pig bionic digestion system provided by the present invention, which is composed of a reaction unit and a cleaning unit;

[0079] Wherein, the reaction unit includes a simulated digester 100, a supporting stirring device 700, a digestion liquid storage bottle 201, a buffer storage bottle 202, a deionized water reagent bottle 203, and a waste liquid collection bottle 204;

[0080] There is at least one simulated digester 100, and the number of the digestive solution storage bottle 201 and the buffer solution storage bottle 202 corresponds to the number of the simulated digester 100;

[0081] Such as Figure 3-4 As shown, the simulated digester 100 is made up of two layers of glass tubes inside and outside. The inner glass tube 101 opens upwards, and the outer glass tube 102 is connected to the upper part of the inner glass tube 101 by shrinking and connecting the inner glass tube 101. The opening of the tube 101 is higher than t...

Embodiment 2

[0106] Determination of the digestible energy value of corn and barley by pig bionic digestion method

[0107] 1. Pretreatment of feed ingredients to be tested

[0108] Sampling according to GB / T 14699.1, using 3 corns (total energy 4444-4475kcal / kg DM, crude protein 7.69%~7.90%, crude fat 2.99%~3.52%, crude ash content 1.09%~1.17%), 2 barley (Total energy 4408~4441kcal / kg DM, crude protein 8.26%~10.01%, crude fat 1.74%~1.90%, crude ash content 1.47%~1.77%) were taken as representative feed samples. Divide the collected feed samples into quarters to about 200g, crush them with a universal pulverizer, pass through a 60-mesh standard sieve, seal them in a sample bag and store at -20°C for later use.

[0109] 2. The bionic digestion process of pigs

[0110] 2.1 Preparation of buffer and simulated digestion:

[0111] Gastric buffer (500mL): Weigh 2.59g of NaCl, 0.25g of KCl, and 6g of anhydrous sodium dihydrogen phosphate. Dissolve in 450mL deionized water, adjust the pH to 2.0 with 2mol...

Embodiment 3

[0181] Determination of digestible energy value of wheat, early rice, sorghum and broken rice by pig bionic digestion method

[0182] Reference Figure 1-6 The in vitro bionic digestion method for rapid determination of digestible energy value of pig feed in this embodiment has the following differences from embodiment 1-6:

[0183] 1 Pretreatment of feed ingredients

[0184] Sampling according to GB / T 14699.1 2 wheat (GE 4460~4493kcal / kg DM, CP 14.25%~15.33%, EE 1.44%~1.68%, Ash 1.41%~1.53%), 1 early rice and 1 broken rice ( GE 4306~4365kcal / kg DM, CP 7.40%~7.66%, EE 0.50%~1.83%, Ash 0.24%~3.34%), 1 sorghum (GE 4476kcal / kg DM, CP 8.46%, EE 3.02%, Ash 1.28 %) and other commonly used pig feed ingredients as representative feed samples. After being crushed by a universal pulverizer and passing through a 60-mesh standard sieve, they were put into feed sample bags and sealed at -20°C for later use.

[0185] 2 Pig's bionic digestion process

[0186] Same as Example 2

[0187] 3 Bionic dig...

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Abstract

The invention discloses a program-controlled pig bionic digestion system and a method using the program-controlled pig bionic digestion system to quickly determining a digestible energy value of pig feed. The method comprises the following steps that a feed sample is pulverized and sieved through a standard sieve; a gastric buffer solution and an intestinal buffer solution are prepared; simulatedgastric juice, simulated small intestinal juice and simulated large intestinal juice are prepared; the pulverized feed sample is filled into a simulated digester of the program-controlled pig bionic digestion system, and the simulated gastric juice is added in sequence through computer program control to simulate gastric digestion; after the intestinal buffer solution is added, the simulated smallintestinal juice is added to simulate small intestinal digestion; the simulated large intestinal juice is added to simulate large intestinal digestion; inactivation is carried out after the digestionis completed; and then cleaning is carried out, after undigested residues are obtained, a total energy value of the residues and the feed sample is tested, and the digestible energy value of the feedis calculated. The method is simple, high in precision, low in implementation cost, and the digestible energy value of the pig feed can be accurately determined within 72 hours.

Description

Technical field [0001] The invention belongs to the field of agriculture, and relates to a method for quickly determining the digestible energy value of pig feed, in particular to an in vitro simulated digestion method and system for quickly determining the digestive energy value of pig feed. Background technique [0002] Digestible energy is the basic parameter to evaluate the feed energy availability of pigs. In the preparation of pig diets, digestible energy is the basic parameter that needs to be met first. The ration is not only related to the production cost of pig compound feed, but also affects the utilization of other nutrients in the diet. Because feed costs account for more than 70% of the total production costs of pigs. Therefore, it is very important to accurately and objectively assess the digestible energy value of feed to increase the profitability of pig breeding. [0003] At present, the digestible energy value of pig feed ingredients is basically based on the ...

Claims

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Application Information

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IPC IPC(8): G01N31/00
CPCG01N31/00
Inventor 赵峰高庆涛王钰明廖睿
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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