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Program-controlled bionic digestive system for pigs and a method for rapidly determining the digestible energy value of pig feed using the system

A digestive system and rapid measurement technology, applied in the agricultural field, can solve the problems of rupture and inability to simulate the digestion of the large intestine, and achieve the effect of high repeatability and fast test speed.

Active Publication Date: 2021-11-16
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when this system simulates the digestion stage of the pig's large intestine, the cellulase that simulates the large intestine fluid can degrade the dialysis bag, causing it to rupture, which cannot truly simulate the digestion of the large intestine

Method used

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  • Program-controlled bionic digestive system for pigs and a method for rapidly determining the digestible energy value of pig feed using the system
  • Program-controlled bionic digestive system for pigs and a method for rapidly determining the digestible energy value of pig feed using the system
  • Program-controlled bionic digestive system for pigs and a method for rapidly determining the digestible energy value of pig feed using the system

Examples

Experimental program
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Effect test

Embodiment 1

[0078] Such as Figure 1-2 Shown is a programmable pig bionic digestive system provided by the present invention, which is composed of a reaction unit and a cleaning unit;

[0079] Wherein, the reaction unit includes a simulated digester 100, a supporting stirring device 700, a digestive liquid storage bottle 201, a buffer storage bottle 202, a deionized water reagent bottle 203, and a waste liquid collection bottle 204;

[0080] There is at least one simulated digester 100, and the number of the digestive solution storage bottle 201 and the buffer storage bottle 202 corresponds to the number of the simulated digester 100;

[0081] Such as Figure 3-4 As shown, the simulated digester 100 is formed by sheathing two layers of glass tubes inside and outside, wherein the inner glass tube 101 opens upwards, and the mouth of the outer glass tube 102 is shrunk and connected to the upper part of the inner glass tube 101, so that the inner glass tube The opening of the tube 101 is hi...

Embodiment 2

[0106] Determination of digestible energy values ​​of maize and barley by porcine biomimetic digestion

[0107] 1. Pretreatment of feed ingredients to be tested

[0108] Sampling was carried out according to GB / T 14699.1, with 3 corns (total energy 4444-4475kcal / kg DM, crude protein 7.69%-7.90%, crude fat 2.99%-3.52%, crude ash 1.09%-1.17%), 2 barley (total energy 4408-4441kcal / kg DM, crude protein 8.26%-10.01%, crude fat 1.74%-1.90%, crude ash 1.47%-1.77%) as a representative feed sample. The collected feed samples were divided into quarters to about 200g, pulverized with a universal grinder and passed through a 60-mesh standard sieve, sealed in sample bags and stored at -20°C for later use.

[0109] 2. Biomimetic digestion process in pigs

[0110] 2.1 Preparation of buffer solution and simulated digestive solution:

[0111] Gastric buffer solution (500mL): Weigh 2.59g of NaCl, 0.25g of KCl, and 6g of anhydrous sodium dihydrogen phosphate. Dissolve in 450mL deionized wate...

Embodiment 3

[0181] Determination of digestible energy values ​​of wheat, early rice, sorghum and broken rice by porcine biomimetic digestion method

[0182] refer to Figure 1-6 , the in vitro biomimetic digestion method of a kind of pig feed digestible energy value rapid determination of the present embodiment has the following difference with embodiment 1-6:

[0183] 1 Pretreatment of feed ingredients

[0184] Sampling according to GB / T 14699.1 2 wheat (GE 4460~4493kcal / kg DM, CP 14.25%~15.33%, EE 1.44%~1.68%, Ash 1.41%~1.53%), 1 early rice and 1 broken rice ( GE 4306~4365kcal / kg DM, CP 7.40%~7.66%, EE 0.50%~1.83%, Ash 0.24%~3.34%), 1 sorghum (GE 4476kcal / kg DM, CP 8.46%, EE 3.02%, Ash 1.28 %) and other commonly used pig feed ingredients as representative feed samples. After crushing with a universal pulverizer, pass through a 60-mesh standard sieve, put them into feed sample bags, seal them, and store them at -20°C for later use.

[0185] 2 Biomimetic digestion process in pigs

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Abstract

The invention discloses a program-controlled pig bionic digestion system and an in vitro simulated digestion method using the system to quickly determine the digestibility value of pig feed, comprising the following steps: crushing feed samples through a standard sieve; preparing gastric buffer and intestinal buffer ; Prepare simulated gastric juice, simulated small intestine juice, and simulated large intestine juice; put the pulverized feed sample into the simulated digester of the programmed pig bionic digestive system, and add simulated gastric juice in sequence through computer program control to simulate gastric digestion; add intestinal buffer Then add simulated small intestine juice to simulate small intestine digestion; add simulated large intestine juice to simulate large intestine digestion; inactivate after digestion; then wash, obtain undigested residues, measure the total energy value of residues and feed samples, and calculate feed Digestive energy value. The method of the invention is simple, high in precision and low in implementation cost, and can accurately measure the digestible energy value of pig feed within 72 hours.

Description

technical field [0001] The invention belongs to the field of agriculture, and relates to a method for quickly measuring the digestible energy value of pig feed, in particular to an in vitro simulated digestion method and system for rapidly measuring the digestible energy value of pig feed. Background technique [0002] Digestible energy is the basic parameter for evaluating the availability of feed energy to pigs. In the formulation of pig diets, digestible energy value is the first basic parameter that needs to be met. The ration is not only related to the production cost of pig compound feed, but also affects the utilization of other nutrients in the diet. Because the cost of feed accounts for more than 70% of the total production cost of live pigs. Therefore, it is very important to accurately and objectively evaluate the digestible energy value of feed to improve the profit of pig farming. [0003] At present, the pig digestible energy value of feed raw materials is b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N31/00
CPCG01N31/00
Inventor 赵峰高庆涛王钰明廖睿
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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