Artificially simulated pig stomach and intestine digestive fluid, preparation method and application of digestive fluid
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A technology of artificial simulation and artificial gastric juice, applied in the biological field, can solve the problems of limited quantity and poor feasibility, and achieve the effect of simple and accurate in vitro evaluation
Active Publication Date: 2015-01-21
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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Problems solved by technology
However, due to the difficulty of obtaining animal digestive juices and the very limited amount obtained, the feasibility of practical application is very poor
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Embodiment 1
[0047] Embodiment 1, preparation of artificial simulated pig stomach digestive juice and artificial simulated pig small intestine digestive juice
[0048] 1. Preparation of artificial gastric juice and artificial small intestinal juice
[0049] 1. Preparation of artificial gastric juice
[0050] Weigh 2g of sodium chloride, 3.2g of pepsin (equivalent to 12160U), measure 7mL of concentrated hydrochloric acid (equivalent to 12mol / L) with a mass fraction of 36.5%, add distilled water to 1000mL, and mix well to obtain the required solution , stored at 4°C.
[0051] 2. Preparation of artificial small intestine juice
[0052] Weigh 6.8g of potassium dihydrogen phosphate, add 500mL of distilled water to dissolve, adjust the pH to 6.8 with 0.1mol / L sodium hydroxide solution, weigh 10g of trypsin (equivalent to trypsin 6000U / L; trypsin 70000U / L; Pancrelipase 40000U / L), add distilled water to dissolve, mix the two liquids, add another 3g of porcine bile salt, add distilled water to d...
Embodiment 2
[0058] Example 2. Evaluation of the in vitro adsorption rate of mycotoxin adsorbents to mycotoxins by artificially simulating pig stomach and small intestine digestive juice
[0059] Mycotoxin to be tested: Zearalenone, CAS No.: 17924-92-4, purity ≥ 99%, Israel Fermentek Company.
[0060] Mycotoxin adsorbents for testing: 8 commercially available montmorillonite mycotoxin adsorbents (montmorillonite 1# to montmorillonite 8# in Table 1 and Table 2) and 5 yeast cell wall mycotoxin adsorbents ( Yeast cell wall 1# to yeast cell wall 5# in Table 1 and Table 2), wherein 8 kinds of montmorillonite mycotoxin adsorbents were purchased from Zhejiang Sanding Technology Co., Ltd., Panzhihua Xingjia Environmental Technology Co., Ltd., Chifeng and Zhengmei Chemical Co., Ltd., Chifeng Wuhua Tianbao Mineral Materials Co., Ltd., Zhejiang Fenghong New Materials Co., Ltd., Shouguang Zhonglian Fine Montmorillonite Co., Ltd., Inner Mongolia Tianyuan Montmorillonite Development Co., Ltd., Inner Mon...
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Abstract
The invention discloses an artificially simulated pig stomach and intestine digestive fluid, a preparation method and application of the digestive fluid. The preparation method comprises the following steps: mixing pig compound feed and artificial gastric fluid according to a ratio of 1g to (3-3.5mL), adjusting the pH to be 1.5-2.0, oscillating at 39+/-1 DEG C for 1-2 hours, cooling to 4+/-1 DEG C, centrifuging 8000-9000g at 4+/-1 DEG C for 5-10 minutes, and taking liquid supernatant as the artificially simulated pig stomach digestive fluid; and adding artificial intestinal fluid which is 3 times of the volume of a sediment obtained by centrifuging, adjusting the pH to be 6.8+/-0.2, oscillating at 39+/-1 DEG C for 1-2 hours, cooling to 4+/-1 DEG C, centrifuging 18000-20000g at 4+/-1 DEG C for 5-10 minutes, and taking liquid supernatant as the artificially simulated pig intestine digestive fluid. According to the artificially simulated pig stomach and intestine digestive fluid, the conditions such as the pH value and the temperature are consistent with the condition of a pig gastrointestinal tract; by virtue of digestion of special feed, the soluble components in the feed are introduced and are relatively consistent with the component of the pig digestive fluid, in the actual production; the scientific, objective and accurate in-vitro evaluation is carried out on adsorption rate of a mycotoxin absorbent in the pig; the method is simple and quick.
Description
technical field [0001] The invention belongs to the field of biotechnology, and relates to an artificially simulated pig stomach and small intestine digestive juice, a preparation method and application thereof. Background technique [0002] Mycotoxin adsorbent is a kind of feed additive with the ability to absorb mycotoxins, which can combine with mycotoxins in feed in the digestive tract of animals, so as to reduce the absorption of mycotoxins in the gastrointestinal tract of animals and increase the excretion of mycotoxins through feces . Among them, the adsorption rate of specific mycotoxins is an important index to evaluate the quality of mycotoxin adsorbents. [0003] At present, there are mainly two methods for evaluating the adsorption rate of mycotoxin adsorbents: in vitro method and in vivo method. The in vitro method generally uses phosphate buffer with different pH values as the medium to determine the adsorption capacity of the adsorbent for a certain mycoto...
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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 王瑞国苏晓鸥樊霞王培龙张维高忠武张瑜
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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