3 results about "Rna hybridization" patented technology

The present application relates to the technical field of biological medicine, and in particular to a molecular marker of alveolar rhabdomyosarcoma and application thereof, wherein the molecular marker is circFOXO1R-loop site, and a DNA-RNA hybridization region thereof is located at chr13:41133915-41134263 of a human genome. In the present application, the circFOXO1R-loop site is verified by CUT&Tag-qPCR and DRIP-qPCR, and it is found that the circFOXO1R-loop can promote the combination of FOXO1 gene and RNAPol II and DNA damage, and it has important clinical application value for developing a reagent or kit for diagnosing alveolar rhabdomyosarcoma, a drug or a gene therapy strategy for preventing and / or treating alveolar rhabdomyosarcoma aiming at the target.

This invention relates to an in situ multi-throughput glycosylated RNA imaging method, comprising the following steps: constructing different proxHCR systems for different glycoRNAs, thereby performing multi-throughput in situ imaging of multiple glycoRNAs with the same sialic acid composition but different RNA sequences; the proxHCR system includes a sialic acid recognition module, an RNA hybridization module, and a fluorescently labeled hairpin probe (AR) acting as an amplification reporter. The sialic acid recognition module and the proxHCR module are used for dual recognition of sialic acid and RNA units of glycoRNAs on the cell membrane surface. A complete activation foothold switch is formed only when the sialic acid and RNA units of the glycoRNAs on the cell membrane surface are spatially adjacent, triggering HCR of the fluorescently labeled hairpin probe (AR), thereby visualizing the glycoRNAs on the cell surface through amplified fluorescence signals. This invention solves the problem of the lack of prior art for simultaneous multi-throughput analysis of multiple different glycosylated RNAs.

The invention provides a method for measuring the positive rate of cells expressing exogenous therapeutic genes by combining RNA (Ribonucleic Acid) hybridization and flow cytometry. The detection method comprises the following steps: designing a specific in-situ hybridization probe aiming at mRNA (Messenger Ribonucleic Acid) expressed by a target gene, carrying out RNA probe in-situ hybridization staining in the cells, and distinguishing specific cell subgroups by combining the single cell resolution of flow cytometry. A feasible detection method is provided for highly personalized and multivalent TCR and other cell treatment samples which do not have specific antibodies or cannot detect the positive rate by using a unique antibody, and the design space for designing the specific target RNA probe is larger and the difficulty is lower than that for designing the specific antibody. Comprising the following steps: fixing cell morphology and RNA, permeabilizing / breaking a cell membrane, fixing / perforating a cell nucleus, digesting intracellular and intracellular free single-stranded DNA, carrying out RNA probe hybridization and signal amplification, and carrying out flow detection.