Moisturizing anti-aging nanoemulsion, preparation method thereof and moisturizing essence

Abstract

The invention relates to the technical field of cosmetics, in particular to moisturizing and anti-aging nanoemulsion, a preparation method thereof and moisturizing essence. The moisturizing and anti-aging nanoemulsion comprises the following raw materials in percentage by mass: 90-99% of an anti-aging component and 1-10% of a moisturizing component, and the anti-aging component comprises the following raw materials in percentage by mass: 1-20% of a glycosylglycerol solution, 0.5-3% of nicotinamide adenine dinucleotide, 0.1-5% of sodium coramate and the balance of a solvent; the moisturizing component comprises ceramide lipidosome. The raw materials in the moisturizing and anti-aging nanoemulsion synergistically exert effects, the moisturizing and anti-aging effects of the moisturizing and anti-aging nanoemulsion are improved, meanwhile, the stability and applicability of the moisturizing and anti-aging nanoemulsion are enhanced, and the moisturizing and anti-aging nanoemulsion is mild and non-irritant to skin and safe to use. The preparation method of the moisturizing anti-aging nanoemulsion is simple in process, high in production efficiency and beneficial to industrial production. The moisturizing essence has more remarkable moisturizing and anti-aging effects by adding the moisturizing and anti-aging nanoemulsion, and is safe to use.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a moisturizing and anti-aging nanoemulsion, a preparation method thereof, and a moisturizing essence. Background Art

[0002] Skin moisture is crucial for maintaining its health and appearance. With aging, environmental factors, and lifestyle influences, skin moisture loss gradually worsens, leading to deterioration of skin function and symptoms such as dryness, roughness, and loss of elasticity. Furthermore, moisture loss impairs the synthesis and maintenance of collagen and elastin fibers. Collagen is a key component in maintaining skin's elasticity and firmness, while elastin fibers provide the skin with its resilience and flexibility. Damage to these fibers results in a loss of elasticity and firmness, leading to increased wrinkles and sagging.

[0003] The moisturizing and anti-aging products currently on the market generally have limitations: single moisturizing products rely solely on ingredients such as glycerin and hyaluronic acid to form a water-locking film on the surface of the stratum corneum, and lack intervention in the collagen metabolism and barrier repair mechanism of the dermis; and products claiming to be anti-wrinkle are mostly based on retinol and ordinary peptides. Although they can stimulate collagen synthesis in the short term, they ignore the vicious cycle of water loss and barrier damage. In addition, existing technologies use ordinary emulsification processes to reduce particle size, but due to process limitations, the particle size reduction is limited and the stability is poor, which still cannot meet the needs of deep penetration. Moreover, most products have not built a synergistic pathway of "moisturizing-repairing-anti-aging", and cannot simultaneously solve multiple aging factors such as water loss, barrier damage, and collagen degradation, making it difficult to achieve comprehensive intervention in skin aging.

[0004] Therefore, developing a composition that can improve the penetration efficiency of ingredients through a nanoscale delivery system, while also having deep moisturizing, barrier repair and full-layer anti-wrinkle functions, in order to break through the existing technical bottleneck and meet the market's urgent demand for efficient and long-lasting moisturizing and anti-aging solutions, has important scientific significance and industrial value. Summary of the Invention

[0005] In order to overcome the shortcomings and deficiencies in the prior art, the present invention aims to provide a moisturizing anti-aging nanoemulsion and a preparation method thereof. The moisturizing anti-aging nanoemulsion is compounded with raw materials such as glycerol glucoside, nicotinamide adenine dinucleotide, sodium colanate, and ceramide liposomes. The raw materials work synergistically to improve the moisturizing and anti-aging effects of the moisturizing anti-aging nanoemulsion, while enhancing its stability and applicability. It is mild and non-irritating to the skin and safe to use. The preparation method of the moisturizing anti-aging nanoemulsion is simple in process, high in production efficiency, and conducive to industrial production.

[0006] Another object of the present invention is to provide a moisturizing essence, which has more significant moisturizing and anti-aging effects by adding the above-mentioned moisturizing and anti-aging nanoemulsion, can effectively reduce the loss of skin moisture, keep the skin hydrated and elastic, delay skin aging, and is safe to use.

[0007] The objective of the present invention is achieved through the following technical solution: a moisturizing and anti-aging nanoemulsion, comprising the following raw materials in mass percentage: 90-99% of an anti-aging component and 1-10% of a moisturizing component, wherein the anti-aging component comprises the following raw materials in mass percentage: 1-20% of a glycerol glucoside solution, 0.5-3% of nicotinamide adenine dinucleotide, 0.1-5% of sodium colanate, and the remainder being a solvent; the moisturizing component comprises ceramide liposomes.

[0008] The present invention provides a moisturizing and anti-aging nanoemulsion comprising an anti-aging component and a moisturizing component in a specific ratio, wherein the anti-aging component is organically combined with glycerol glucoside, nicotinamide adenine dinucleotide, sodium colanate and a solvent, and is further combined with ceramide liposomes, which are moisturizing components. The synergistic effect of each component produces effects in skin keratinocytes, epidermis, epidermal junction layer and dermis, respectively. Glycerol glucoside increases AQP3 expression and enhances hydration, ceramide liposomes replenish stratum corneum lipids, and sodium colanate upregulates filaggrin, significantly increasing the water content of the stratum corneum and reducing It reduces transepidermal water loss, establishes moisturizing and barrier repair; at the same time, nicotinamide adenine dinucleotide and sodium colanate synergistically accelerate the skin's own synthesis of NAD+, promote mitochondrial autophagy, upregulate antioxidant genes, delay cell aging, promote the production of type I and type III collagen and elastin, inhibit matrix degrading enzymes, improve wrinkles and skin elasticity, and form a synergistic mechanism of "moisturizing-repairing-anti-aging", thereby improving the moisturizing and anti-aging effect of the moisturizing and anti-aging nanoemulsion and enhancing its stability and applicability, providing consumers with an efficient, convenient and safe skin care product.

[0009] Furthermore, the molecular weight of sodium colanate is 20-5000kDa. Sodium colanate is an extracellular anionic polysaccharide synthesized from the stress response of probiotics. It targets mitochondria and activates the TBK1 and BNIP3 pathways to promote mitochondrial autophagy and the unfolded protein response to maintain mitochondrial homeostasis. It also upregulates NAMPT activity to enhance NAD+ and ATP production, and regulates mitochondrial function by reducing ROS levels through genes such as SOD2 and GPX1 / 8. In the dermis, it promotes the production of type I and type III collagen and elastin, inhibits proteases such as MMP9, and promotes the synthesis of extracellular matrix such as fibronectin to combat wrinkles and repair wrinkles. By inhibiting MMP9, it increases the standardized area of ​​the dermoid junction (DEJ) layer to strengthen the epidermal junction. In the epidermis, it increases filaggrin content to enhance the barrier, upregulates AQP3 to promote hydration, and inhibits inflammatory factors such as TNF-α to provide anti-inflammatory and soothing effects. Furthermore, sodium colanate has high transdermal absorption capacity, penetrating the skin barrier and entering the mitochondria to exert its anti-wrinkle effect.

[0010] Furthermore, the ceramide liposomes are composed of five ceramides: ceramide NP, ceramide EOP, ceramide NS / ceramide NG, ceramide AS, and ceramide AP, as well as excipients: hydrogenated lecithin and glycerol. The ceramide liposomes comprise the following raw materials in percentage by weight: 4-5% ceramide NP, 0.000001-0.01% ceramide EOP, 0.01-0.2% ceramide NS / ceramide NG, 0.01-0.2% ceramide AS, 0.000001-0.01% ceramide AP, 20-30% hydrogenated lecithin, and the balance glycerol. Ceramide is a sphingolipid composed of a long-chain sphingosine base and fatty acids. It is a major component of cell membranes, particularly in the stratum corneum, accounting for 40-50% of the total. Ceramide plays an important role in maintaining water balance in the stratum corneum. It has a strong ability to bind water molecules and maintains skin moisture by forming a network structure in the stratum corneum. Ceramides can also penetrate the stratum corneum, replenishing intercellular lipids and strengthening the skin's barrier function. Furthermore, ceramide NP, a major component of intercellular lipids in the stratum corneum, can directly replenish stratum corneum lipids and enhance barrier function. Ceramide EOP promotes stratum corneum cell maturation and improves barrier integrity. Ceramides NS / NG, AS, and AP work synergistically to maintain the stability of the stratum corneum's lipid structure and further enhance moisture retention.

[0011] Furthermore, each glycerol glucoside solution comprises the following raw materials in the following percentages by weight: 45-55% glycerol glucoside, 3-7% 1,2-pentanediol, and 40-50% water. The glycerol glucoside is a glycoside compound formed by linking one molecule of glycerol and one molecule of glucose via a glycosidic bond. The glycerol glucoside of the present invention is synthesized using deep catalytic fermentation of genetically engineered bacteria to form a 2-α-GG configuration. It can significantly increase the expression of the aquaporin AQP3, which plays an important role in the water retention function of skin epithelial cells. Furthermore, the glycerol glucoside of the present invention has good skin permeability and can effectively reduce skin water loss.

[0012] Furthermore, the solvent is at least one of ionized water and alcohol solvents; the alcohol solvent is at least one of 1,3-propylene glycol, butylene glycol, dipropylene glycol, 1,2-hexanediol and glycerol.

[0013] Furthermore, each portion of the solvent comprises the following raw materials in percentage by mass: 3-6% butanediol, 1-3% 1,2-hexanediol, and the balance water.

[0014] The present invention also provides a method for preparing a moisturizing and anti-aging nanoemulsion, comprising the following steps:

[0015] (1) Preparing a moisturizing component: ceramide NP, ceramide EOP, ceramide NS / ceramide NG, ceramide AS, ceramide AP, hydrogenated lecithin, and glycerol were placed in an emulsifying pot, heated to 60-80°C, and homogenized at a speed of 15,000-28,000 r / min for 3-8 minutes. After homogenization, the mixture was transferred to a microfluidizer and homogenized at a pressure of 500-700 bar and a temperature of 40-80°C for 2-4 cycles to obtain a moisturizing component;

[0016] (2) Preparing an anti-aging component: putting glycerol glucoside, nicotinamide adenine dinucleotide, sodium colanate and a solvent into a stirring pot, stirring and dissolving them uniformly, and then transferring them into an ultrasonic treatment pot, performing low-temperature ultrasonic treatment at a temperature of 25-40° C. for 10 min-30 min, wherein the ultrasonic frequency is 20 kHz-40 kHz, to obtain an anti-aging component;

[0017] (3) The anti-aging component and the moisturizing component are mixed and stirred evenly, and transferred to a microfluidizer, and the mixture is homogenized at a pressure of 600-900 bar, a temperature of 40-80° C., and a cycle number of 3-6 times to obtain a moisturizing anti-aging nanoemulsion.

[0018] The preparation method of the moisturizing, anti-aging nanoemulsion of the present invention is simple, highly efficient, and conducive to industrial production. The moisturizing component is prepared by a microfluidization process, and then the particle size of the moisturizing component is refined and mixed with the anti-aging component to produce the moisturizing, anti-aging nanoemulsion. The moisturizing, anti-aging nanoemulsion produced by the two microfluidization processes is a nanoliposome with a particle size of 50-100 nm, which improves skin absorption and system stability, ensuring long-term efficacy release.

[0019] The moisturizing and anti-aging nanoemulsion provided by the present invention can be applied to various skin care products, including creams, lotions, essences, masks or serums. The percentage content of the moisturizing and anti-aging nanoemulsion in the skin care products is 2%-8%.

[0020] The present invention also provides a moisturizing essence comprising the moisturizing and anti-aging nanoemulsion.

[0021] Furthermore, the moisturizing essence comprises the following raw materials in percentage by mass: 4-6% moisturizing and anti-aging nanoemulsion, 2-6% glycerol, 0.8-1.2% glycerol polyether, 0.05-0.15% xanthan gum, 0.1-0.3% carbomer, 0.1-0.3% arginine, 2-4% butylene glycol, 0.2-0.6% 1,2-hexanediol, 0.2-0.6% p-hydroxyacetophenone, and the balance water.

[0022] Furthermore, the glycerol polyether is glycerol polyether-26. The glycerol polyether uses glycerol polyether-26, which is an excellent moisturizer that can quickly replenish moisture to the skin and form a lubricating protective film on the skin surface. This film can reduce water loss from the skin surface while making the skin feel soft and smooth. Its moisturizing effect is long-lasting and can keep the skin hydrated for a long time.

[0023] The present invention also provides a method for preparing the above-mentioned moisturizing essence, comprising the following steps:

[0024] (S1) adding a portion of water to a container, then adding a pre-mixed mixture of glycerol, glycerol polyether, xanthan gum, and carbomer, stirring, then heating to 80-90° C., and homogenizing until there are no particles, so that all the raw materials are evenly dispersed, to obtain phase A;

[0025] (S2) adding arginine to a small amount of water and stirring to dissolve to obtain phase B;

[0026] (S3) mixing p-hydroxyacetophenone, 1,2-hexanediol, and butanediol and heating to 60-80° C. to uniformly disperse the raw materials to obtain phase C;

[0027] (S4) adding phase B and phase C to phase A cooled to 50-70°C, mixing uniformly, and obtaining a mixed solution;

[0028] (S5) Cooling the mixed solution to below 40° C., adding the moisturizing and anti-aging nanoemulsion and mixing evenly to obtain a moisturizing essence.

[0029] Furthermore, in step S2, the amount of water added is 3-8% by mass of the moisturizing essence.

[0030] The beneficial effects of the present invention are as follows: the moisturizing and anti-aging nanoemulsion of the present invention is through the anti-aging component and the moisturizing component in a specific ratio, wherein the anti-aging component is organically combined by glycerol glucoside, nicotinamide adenine dinucleotide, sodium colanate and a solvent, and is further matched with the ceramide liposome of the moisturizing component, so that each raw material is well matched and synergistically exerts its efficacy. The moisturizing and anti-aging nanoemulsion can act on mitochondria, optimize the signal transmission and material transmission between the dermis and the epidermis, keep the skin moisturized and elastic, and delay the pace of aging from multiple dimensions. It is mild and non-irritating to the skin and safe to use. The preparation method of the moisturizing and anti-aging nanoemulsion is simple in process, high in production efficiency, and conducive to industrial production. The moisturizing and anti-aging nanoemulsion of the present invention can be applied to skin care products such as moisturizing essences, which can make the moisturizing essences have more significant moisturizing and anti-aging effects. Long-term use of the skin care products containing the present invention can effectively reduce the loss of skin moisture, keep the skin moisturized and elastic, delay the aging of the skin, and is safe to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 14 d after using the moisturizing essence prepared in Example 2.

[0032] Figure 2 Comparison of the baseline transepidermal water loss (D0 before using the test sample) and the measured value after using the moisturizing essence prepared in Example 2 for 14 days.

[0033] Figure 3 This is a comparison chart of the basic value of the F4 value characterizing skin firmness (D0 before using the test sample) and the measured value 14 days after using the moisturizing essence prepared in Example 2.

[0034] Figure 4 3. This is a comparison chart of the skin elasticity characterization value R2 basic value (D0 before using the test sample) and the measured value after using the moisturizing essence prepared in Example 2 for 14 days. DETAILED DESCRIPTION

[0035] In order to facilitate understanding by those skilled in the art, the present invention will be further described below with reference to embodiments and drawings. The contents mentioned in the embodiments are not intended to limit the present invention.

[0036] Example 1

[0037] In this embodiment, a moisturizing and anti-aging nanoemulsion includes the following raw materials in mass percentage: 97% anti-aging component and 3% moisturizing component, wherein the anti-aging component includes the following raw materials in mass percentage: 5% glycerol glucoside solution, 0.5% nicotinamide adenine dinucleotide, 1% sodium colanate, 5% butylene glycol, 2% 1,2-hexanediol, and the balance water; the moisturizing component is ceramide liposomes.

[0038] The ceramide liposomes include the following raw materials in percentage by weight: ceramide NP 5%, ceramide EOP 0.000001%, ceramide NS / ceramide NG 0.1%, ceramide AS 0.1%, ceramide AP 0.000001%, hydrogenated lecithin 25%, and the balance glycerol.

[0039] Each glycerol glucoside solution contains the following raw materials in percentage by weight: glycerol glucoside 48%, 1,2-pentanediol 5%, water 47%

[0040] In this embodiment, a method for preparing a moisturizing and anti-aging nanoemulsion comprises the following steps:

[0041] (1) Preparation of moisturizing component: Ceramide NP, ceramide EOP, ceramide NS / ceramide NG, ceramide AS, ceramide AP, hydrogenated lecithin and glycerol were placed in an emulsifying pot, heated to 80°C, homogenized at a speed of 21500 r / min for 5 min, and then transferred to a microfluidizer after homogenization, and the mixture was subjected to a pressure of 600 bar and a temperature of 60°C for 3 cycles to obtain a moisturizing component;

[0042] (2) Preparation of anti-aging components: Glycerol glucoside, nicotinamide adenine dinucleotide, sodium colanate and solvent were placed in a stirring pot, stirred and dissolved uniformly, and then transferred to an ultrasonic treatment pot and subjected to low-temperature ultrasonic treatment at a temperature of 30°C for 25 minutes, wherein the ultrasonic frequency was 33 kHz, to obtain the anti-aging components;

[0043] (3) The anti-aging component and the moisturizing component were mixed and stirred evenly, and then transferred to a microfluidizer, and the mixture was circulated for 3 times at a pressure of 750 bar and a temperature of 50° C. to obtain a moisturizing anti-aging nanoemulsion.

[0044] This embodiment also provides a moisturizing essence, which includes the following raw materials in parts by weight: 5% moisturizing and anti-aging nanoemulsion prepared in this embodiment, 4% glycerol, 1% glycerol polyether, 0.06% xanthan gum, 0.2% carbomer, 0.2% arginine, 3% butylene glycol, 0.4% 1,2-hexanediol, 0.4% p-hydroxyacetophenone, and the balance water.

[0045] In this embodiment, the glycerol polyether is glycerol polyether-26.

[0046] In this embodiment, the preparation method of the moisturizing essence comprises the following steps:

[0047] (S1) adding a portion of water to a container, then adding a pre-mixed mixture of glycerol, glycerol polyether, xanthan gum, and carbomer, stirring, then heating to 85° C., and homogenizing for 3 minutes until there are no particles, so that all the raw materials are evenly dispersed, to obtain phase A;

[0048] (S2) adding arginine to a small amount of water and stirring to dissolve to obtain phase B;

[0049] (S3) mixing p-hydroxyacetophenone, 1,2-hexanediol, and butanediol and heating to 70° C. to uniformly disperse the raw materials to obtain phase C;

[0050] (S4) adding phase B and phase C to phase A cooled to 60°C, mixing well, and obtaining a mixed solution;

[0051] (S5) Cooling the mixed solution to below 40° C., adding the moisturizing and anti-aging nanoemulsion and mixing evenly to obtain a moisturizing essence.

[0052] In this embodiment, in step S2, the amount of water added is 5% by mass of the moisturizing essence.

[0053] Example 2

[0054] In this embodiment, a moisturizing and anti-aging nanoemulsion includes the following raw materials in mass percentage: 97% anti-aging component and 3% moisturizing component, wherein the anti-aging component includes the following raw materials in mass percentage: 10% glycerol glucoside solution, 1% nicotinamide adenine dinucleotide, 2% sodium colanate, 5% butylene glycol, 2% 1,2-hexanediol, and the balance water; the moisturizing component is ceramide liposomes.

[0055] The ceramide liposomes include the following raw materials in percentage by weight: ceramide NP 5%, ceramide EOP 0.000001%, ceramide NS / ceramide NG 0.1%, ceramide AS 0.1%, ceramide AP 0.000001%, hydrogenated lecithin 25%, and the balance glycerol.

[0056] This embodiment also provides a moisturizing essence, which includes the following raw materials in parts by weight: 5% moisturizing and anti-aging nanoemulsion prepared in this embodiment, 4% glycerol, 1% glycerol polyether, 0.06% xanthan gum, 0.2% carbomer, 0.2% arginine, 3% butylene glycol, 0.4% 1,2-hexanediol, 0.4% p-hydroxyacetophenone, and the balance water.

[0057] The rest of the contents of this embodiment are the same as those of Embodiment 1 and will not be repeated here.

[0058] Example 3

[0059] In this embodiment, a moisturizing and anti-aging nanoemulsion includes the following raw materials in mass percentage: 96% anti-aging component and 4% moisturizing component, wherein the anti-aging component includes the following raw materials in mass percentage: 15% glycerol glucoside solution, 3% nicotinamide adenine dinucleotide, 5% sodium colanate, 5% butylene glycol, 2% 1,2-hexanediol, and the balance water; the moisturizing component is ceramide liposomes.

[0060] The ceramide liposomes include the following raw materials in percentage by weight: ceramide NP 5%, ceramide EOP 0.000001%, ceramide NS / ceramide NG 0.1%, ceramide AS 0.1%, ceramide AP 0.000001%, hydrogenated lecithin 25%, and the balance glycerol.

[0061] This embodiment also provides a moisturizing essence, which includes the following raw materials in parts by weight: 5% moisturizing and anti-aging nanoemulsion prepared in this embodiment, 4% glycerol, 1% glycerol polyether, 0.06% xanthan gum, 0.2% carbomer, 0.2% arginine, 3% butylene glycol, 0.4% 1,2-hexanediol, 0.4% p-hydroxyacetophenone, and the balance water.

[0062] The rest of the contents of this embodiment are the same as those of Embodiment 1 and will not be repeated here.

[0063] Comparative Example 1

[0064] The difference between this comparative example and Example 2 is that: in this comparative example, a moisturizing anti-aging nanoemulsion comprises the following raw materials in mass percentage: 97% anti-aging component and 3% moisturizing component, wherein the anti-aging component comprises the following raw materials in mass percentage: 1% nicotinamide adenine dinucleotide, 2% sodium colanate, 5% butanediol, 2% 1,2-hexanediol, and the balance water; the moisturizing component is ceramide liposome.

[0065] The ceramide liposomes include the following raw materials in percentage by weight: ceramide NP 5%, ceramide EOP 0.000001%, ceramide NS / ceramide NG 0.1%, ceramide AS 0.1%, ceramide AP 0.000001%, hydrogenated lecithin 25%, and the balance glycerol.

[0066] The present comparative example also provides a moisturizing essence, which comprises the following raw materials by weight: the moisturizing anti-aging nanoemulsion prepared in the present comparative example 5%, glycerol 4%, glycerol polyether 1%, xanthan gum 0.06%, carbomer 0.2%, arginine 0.2%, butanediol 3%, 1,2-hexanediol 0.4%, p-hydroxyacetophenone 0.4%, and water in the remainder.

[0067] Comparative Example 2

[0068] The present comparative example differs from Example 2 in that in the present comparative example, a moisturizing anti-aging nanoemulsion comprises the following raw materials by mass percentage: an anti-aging component 97% and a moisturizing component 3%, wherein the anti-aging component comprises the following raw materials by mass percentage: glycerol glucoside solution 5%, sodium colate 1%, butanediol 5%, 1,2-hexanediol 2%, and water in the remainder; and the moisturizing component is a ceramide liposome.

[0069] The ceramide liposome comprises the following raw materials by mass percentage: ceramide NP 5%, ceramide EOP 0.000001%, ceramide NS / ceramide NG 0.1%, ceramide AS 0.1%, ceramide AP 0.000001%, hydrogenated lecithin 25%, and glycerol in the remainder.

[0070] The present comparative example also provides a moisturizing essence, which comprises the following raw materials by weight: the moisturizing anti-aging nanoemulsion prepared in the present comparative example 5%, glycerol 4%, glycerol polyether 1%, xanthan gum 0.06%, carbomer 0.2%, arginine 0.2%, butanediol 3%, 1,2-hexanediol 0.4%, p-hydroxyacetophenone 0.4%, and water in the remainder.

[0071] Comparative Example 3

[0072] The present comparative example differs from Example 2 in that in the present comparative example, a moisturizing anti-aging nanoemulsion comprises the following raw materials by mass percentage: an anti-aging component 96% and a moisturizing component 4%, wherein the anti-aging component comprises the following raw materials by mass percentage: glycerol glucoside solution 10%, nicotinamide adenine dinucleotide 1%, butanediol 5%, 1,2-hexanediol 2%, and water in the remainder; and the moisturizing component is a ceramide liposome.

[0073] The ceramide liposome comprises the following raw materials by mass percentage: ceramide NP 5%, ceramide EOP 0.000001%, ceramide NS / ceramide NG 0.1%, ceramide AS 0.1%, ceramide AP 0.000001%, hydrogenated lecithin 25%, and glycerol in the remainder.

[0074] This comparative example also provides a moisturizing essence, which comprises the following raw materials in parts by weight: 5% of the moisturizing and anti-aging nanoemulsion prepared in this comparative example, 4% of glycerol, 1% of glycerol polyether, 0.06% of xanthan gum, 0.2% of carbomer, 0.2% of arginine, 3% of butylene glycol, 0.4% of 1,2-hexanediol, 0.4% of p-hydroxyacetophenone, and the balance of water.

[0075] Comparative Example 4

[0076] The difference between this comparative example and Example 2 is that: a moisturizing anti-aging nanoemulsion comprises the following raw materials in mass percentage: 100% anti-aging component, that is, no moisturizing component is added, wherein the anti-aging component comprises the following raw materials in mass percentage: 10% glycerol glucoside solution, 1% nicotinamide adenine dinucleotide, 2% sodium colanate, 5% butylene glycol, 2% 1,2-hexanediol, and the balance water.

[0077] This comparative example also provides a moisturizing essence, which comprises the following raw materials in parts by weight: 5% of the moisturizing and anti-aging nanoemulsion prepared in this comparative example, 4% of glycerol, 1% of glycerol polyether, 0.06% of xanthan gum, 0.2% of carbomer, 0.2% of arginine, 3% of butylene glycol, 0.4% of 1,2-hexanediol, 0.4% of p-hydroxyacetophenone, and the balance of water.

[0078] In the present invention, the mass percentage of each raw material in the moisturizing and anti-aging nanoemulsions prepared in Examples 1-3 and Comparative Examples 1-4 is shown in Table 1 below:

[0079] Table 1 The dosage ratio of each raw material in the moisturizing and anti-aging nanoemulsion

[0080]

[0081] The mass percentages of the various raw materials of the ceramide liposomes in the moisturizing components prepared in Examples 1-3 and Comparative Examples 1-3 are shown in Table 2 below:

[0082] Table 2 The dosage ratio of each raw material in ceramide liposome

[0083]

[0084]

[0085] The moisturizing and anti-aging nanoemulsions in Examples 1-3 and Comparative Examples 1-4 were added to the essence system. The mass percentages of the raw materials in the prepared moisturizing essences are shown in Table 3 below:

[0086] Table 3 The dosage ratio of each raw material in the moisturizing essence

[0087]

[0088] The moisturizing and anti-aging nanoemulsion and moisturizing essence of the present invention were tested for their effects using the following method:

[0089] 1. Test on the firming effect of the moisturizing and anti-aging nanoemulsions prepared in Examples 1-3 and Comparative Examples 1-4

[0090] (1) Experimental purpose: To investigate the inhibitory effect of moisturizing and anti-aging nanoemulsion on elastase.

[0091] (2) Experimental principle: Elastic fibers are composed of elastin and microfibrils, distributed in the dermis and subcutaneous tissue, and give the skin elasticity. However, environmental factors such as ultraviolet radiation, pressure, and pollution can promote the production of elastase in the body. Elastase belongs to the chymosin family and can degrade elastin, leading to the loss of epidermal connective tissue, and then causing skin aging, wrinkles and photoaging. Studies have shown that inhibiting elastase from decomposing elastin can have a firming effect, so the elastin inhibition rate can reflect the anti-wrinkle performance of the product. Through experimental design, the elastase inhibition rate of the test sample is compared with that of the blank control group. If the inhibition rate of the test sample is better than (higher than) the blank control group and the statistical difference P value is <0.05, it is considered that the test sample has a certain firming effect.

[0092] (3) Experimental method: Add appropriate amount of buffer solution, sample diluent of different concentrations, and substrate to a 96-well plate, shake and mix thoroughly, and incubate for 20 minutes. Then add elastase solution and immediately shake and incubate for 10 minutes. Measure the absorbance at 410 nm using a microplate reader. Analyze the data and calculate the inhibition rate of the sample to elastase. The calculation formula is as follows:

[0093] Elastase inhibition rate (%) = [1-(AB) / (CD)]*100%

[0094] Where:

[0095] A is the absorbance of the experimental group

[0096] B is the absorbance of the experimental blank group

[0097] C is the absorbance of the control group

[0098] D is the absorbance of the blank group

[0099] (4) The specific test results are shown in Table 4:

[0100] Table 4 Test results of the inhibition rate of elastase by the moisturizing and anti-aging nanoemulsions prepared in Examples 1-3 and Comparative Examples 1-4

[0101] Group Elastase inhibition rate (%) Blank group 0.63 Example 1 35.65 Example 2 40.88 Example 3 51.76 Comparative Example 1 32.47 Comparative Example 2 26.67 Comparative Example 3 34.22 Comparative Example 4 14.23

[0102] As can be seen from Table 4 above, the inhibition rate of the moisturizing and anti-aging nanoemulsions of Examples 1-3 on elastase is better than that of Comparative Examples 1-4; it is obvious that the combination of the moisturizing component and the anti-aging component has a synergistic effect.

[0103] 2. Human efficacy test of the moisturizing essence prepared in Example 2

[0104] (1) Test purpose: To evaluate the moisturizing and anti-aging efficacy of the test sample by having volunteers continuously use the moisturizing essence prepared in Example 2 under normal conditions;

[0105] (2) Test method:

[0106] Number of subjects: 35;

[0107] Gender of subjects: 5 males and 30 females;

[0108] Facial skin quality of the subject: dry, loose, rough skin, use Tewameter to test the face, the value is >15g / m 2 h;

[0109] Product usage frequency: After cleansing the skin, take an appropriate amount of the moisturizing essence prepared in Example 2 and apply it evenly on the facial skin. Gently pat until absorbed. Use the product twice a day, once in the morning and once in the evening, for 14 consecutive days.

[0110] Test sample: the moisturizing essence prepared in Example 2.

[0111] (3) Evaluation method: Before using the test sample (D0) and 14 days after using the test sample (D14), the subjects were tested for the moisture content of the stratum corneum, transepidermal water loss, skin firmness F4 value, and skin elasticity R2 value. The test results before and after product use were compared using statistical test methods to determine whether there was a statistical difference. The test indicators of the moisture content of the stratum corneum, transepidermal water loss, skin firmness F4 value, and skin elasticity R2 value are shown in Table 4:

[0112] Table 5 Detection indicators of skin stratum corneum moisture content, skin transepidermal water loss, skin firmness characterization F4 value and skin elasticity characterization value R2

[0113]

[0114]

[0115]

[0116] (4) Statistical methods: Calculate the mean, standard deviation, maximum, minimum and median of each skin parameter before using the test sample (D0) and 14 days after using the test sample (D14); perform a normal distribution test on the test values. If the significance value of the normality test is p>0.05, the data series obeys the normal distribution. Perform a difference analysis before and after using the sample. When the test data conform to the normal distribution, use the t test to analyze the difference between the two groups of data; when the test data do not conform to the normal distribution, use the rank sum test to analyze the difference between the two groups of data. The statistical methods all use a two-tailed test with a test level of α=0.05. If the significance level of the difference analysis is p<0.050, it indicates that there is a statistically significant difference between the subjects before and after using the sample, indicating that the sample has a corresponding effect; if the significance level of the difference analysis is p≥0.050, it indicates that there is no statistically significant difference between the subjects before and after using the sample, indicating that the sample has no corresponding effect.

[0117] The calculation formula is as follows:

[0118]

[0119] Wherein, T0: the total number of skin basal values ​​of all subjects before using the test samples; T1: the total number of skin parameter values ​​of all subjects T days after using the test samples; n: the number of subjects.

[0120] (5) Testing environment: The test environment is 20℃~22℃, 40%~60%RH, and dynamic monitoring is performed.

[0121] (6) Test results:

[0122] ①Skin stratum corneum moisture content test results are as follows Figure 1 As shown in Table 6:

[0123] Table 6 Test results of moisture content of the skin stratum corneum after using the moisturizing essence prepared in Example 2 (unit: CU)

[0124]

[0125] Note: ① A normality test p-value > 0.050 indicates that the data follow a normal distribution and a paired t-test was used. If the data do not follow a normal distribution, a rank sum test was used. ② A significant p-value < 0.050 indicates that there is a significant difference in the stratum corneum moisture content between the two groups before (D0) and 14 days after (D14) the sample is used. A significant p-value ≥ 0.050 indicates that there is no significant difference in the stratum corneum moisture content between the two groups before (D0) and 14 days after (D14) the sample is used.

[0126] Figure 1This is a comparison chart of the baseline moisture content of the stratum corneum (D0 before using the test sample) and the value measured 14 days after using the moisturizing essence prepared in Example 2. (Note: The 14-day measured value was compared with the baseline value. "ns" indicates no significant difference; "*" indicates a significant difference, 0.01≤p<0.05; "**" indicates a significant difference, 0.001≤p<0.01; "***" indicates a significant difference, p<0.001.)

[0127] From Table 6 and Figure 1 It can be seen that after the subjects used the test sample for 14 days, the change rate of the moisture content of the facial skin stratum corneum increased by 12.91% compared with before use, and the moisture content of the facial skin stratum corneum increased significantly (p < 0.001), indicating that the moisturizing essence prepared in Example 2 has a moisturizing effect.

[0128] ② TEWL test results, as shown in Table 7 and Figure 2 As shown:

[0129] Table 7 Transepidermal water loss (TEWL) data after using the moisturizing essence prepared in Example 2 (unit: g / (hm2) 2 ))

[0130]

[0131]

[0132] Note: ① A normality test p-value > 0.050 indicates that the data follow a normal distribution and a paired t-test was used. If the data do not follow a normal distribution, a rank sum test was used. ② A significant p-value < 0.050 indicates that there is a significant difference in the transepidermal water loss (TEWL) values ​​between the two groups before (D0) and 14 days after (D14) treatment with the test sample. A significant p-value ≥ 0.050 indicates that there is no significant difference in the transepidermal water loss (TEWL) values ​​between the two groups before (D0) and 14 days after (D14) treatment with the test sample.

[0133] Figure 2 This is a comparison of the baseline transepidermal water loss value (D0 before using the test sample) and the value measured 14 days after using the moisturizing essence prepared in Example 2. (Note: The 14-day measured value was compared with the baseline value. "ns" indicates no significant difference; "*" indicates a significant difference, 0.01≤p<0.05; "**" indicates a significant difference, 0.001≤p<0.01; "***" indicates a significant difference, p<0.001.)

[0134] From Table 7 and Figure 2It can be seen that after 14 days of use of the test sample, the TEWL value of the subjects improved by 12.69% compared with that before use, and the TEWL value of the facial skin was significantly reduced (p < 0.001), indicating that the moisturizing essence prepared in Example 2 has the effect of reducing TEWL and repairing the skin barrier.

[0135] ③ Skin firmness characterization F4 value test results, as shown in Table 8 and Figure 3 As shown:

[0136] Table 8 F4 value data of skin firmness after using the moisturizing essence prepared in Example 2

[0137]

[0138]

[0139] Note: ① A normality test p-value > 0.050 indicates that the data follow a normal distribution and a paired t-test was used. If the data do not follow a normal distribution, a rank sum test was used. ② A significant p-value < 0.050 indicates that there is a significant difference in the F4 value, a measure of skin firmness, between the data before (D0) and 14 days after (D14) treatment with the test sample. A significant p-value ≥ 0.050 indicates that there is no significant difference in the F4 value, a measure of skin firmness, between the data before (D0) and 14 days after (D14) treatment with the test sample.

[0140] Figure 3 This is a comparison of the baseline F4 value (before application of the test sample, D0) for skin firmness and the value measured 14 days after application of the moisturizing essence prepared in Example 2. (Note: The 14-day measured value was compared with the baseline value. "ns" indicates no significant difference; "*" indicates a significant difference, 0.01≤p<0.05; "**" indicates a significant difference, 0.001≤p<0.01; "***" indicates a significant difference, p<0.001.)

[0141] From Table 8 and Figure 3 As can be seen from the results, the F4 value, a measure of facial skin firmness, improved by 17.66% after 14 days of use compared to before use. The F4 value, a measure of facial skin firmness, decreased significantly (p < 0.001), indicating that the moisturizing essence prepared in Example 2 has a skin-tightening effect.

[0142] ④ Skin elasticity characterization value R2 test results, as shown in Table 9 and Figure 4 As shown:

[0143] Table 9 Skin elasticity R2 data after using the moisturizing essence prepared in Example 2 (unit: %)

[0144]

[0145]

[0146] Note: ① A normality test p-value > 0.050 indicates that the data follow a normal distribution and a paired t-test was used. If the data do not follow a normal distribution, a rank sum test was used. ② A significant p-value < 0.050 indicates that there is a significant difference in the skin elasticity R2 between the two groups before (D0) and 14 days after (D14) treatment. A significant p-value ≥ 0.050 indicates that there is no significant difference in the skin elasticity R2 between the two groups before (D0) and 14 days after (D14) treatment.

[0147] Figure 4 This is a comparison chart of the baseline R2 value (D0 of the test sample before use) of skin elasticity and the value measured 14 days after using the moisturizing essence prepared in Example 2. (Note: The 14-day measured value was compared with the baseline value. "ns" indicates no significant difference; "*" indicates a significant difference, 0.01≤p<0.05; "**" indicates a significant difference, 0.001≤p<0.01; "***" indicates a significant difference, p<0.001.)

[0148] From Table 9 and Figure 4 It can be seen that the skin elasticity characterization value R2 of the subjects increased by 16.93% after 14 days of use compared with before use, and the skin elasticity characterization value R2 increased significantly (p < 0.001), indicating that the moisturizing essence prepared in Example 2 has the effect of improving skin elasticity.

[0149] In summary, after 14 days of use, the moisturizing essence prepared in Example 2 showed a significant increase in the moisture content of the stratum corneum and the skin elasticity value R2 compared to before use, while the transepidermal water loss (TEWL) value and the skin firmness value F4 value were significantly reduced. It can be seen that the moisturizing and anti-aging nanoemulsion of the present invention is applied to skin care products, that is, the moisturizing essence prepared can not only effectively moisturize but also tighten the skin, thereby achieving the effect of delaying skin aging.

[0150] 3. Safety test of the moisturizing essence prepared in Example 2

[0151] (1) Based on the 2015 Cosmetic Safety Technical Specifications, the moisturizing essence prepared in Example 2 was evaluated for cosmetic irritation. The test method was a skin patch test, and 33 randomly distributed people aged 16-65 years were tested.

[0152] (2) Test method: The test substance was placed in a patch tester, and the amount was 0.020-0.025 g. The patch tester with the test substance was covered on the back or the curved side of the forearm of the subject with a non-irritating cloth-based adhesive tape, and the skin surface was uniformly attached by pressing with the palm for 24 hours. After 30 minutes of removing the test patch, the skin reaction was observed after the pressure mark disappeared. If the result was negative, the observation was performed again at 24 hours and 48 hours after the patch test.

[0153] Evaluation criteria:

[0154] 0 grade: negative reaction;

[0155] 1 grade: suspicious reaction, only weak erythema;

[0156] 2 grade: weak positive reaction, erythema, infiltration, edema, and papules can be present;

[0157] 3 grade: strong positive reaction, erythema, infiltration, edema, and papules can be present, and the reaction can exceed the test area;

[0158] 4 grade: very strong positive reaction, obvious erythema, severe infiltration, edema, and confluent blisters, and the reaction exceeds the test area.

[0159] (3) The test results are shown in Table 10:

[0160] Table 10 Results of human skin patch test

[0161]

[0162] The above test results show that the composition and the application product thereof provided by the application are mild and non-irritating to the skin, and are safe to use.

[0163] 4. Stability test of the moisturizing essence prepared in Examples 1-3 and Comparative Examples 3-4

[0164] (1) Stability test conditions and methods, as shown in Table 11

[0165] Table 11 Stability test conditions and methods

[0166]

[0167]

[0168] (2) Test results, as shown in Table 12:

[0169] Table 12 Stability test results of the moisturizing essence prepared in Examples 1-3 and Comparative Examples 3-4 (“√” indicates no abnormality)

[0170]

[0171] As shown in Table 12, the results of Examples 1-2 were normal under all test conditions. The colors of Example 3 and Comparative Examples 3-4 became slightly darker under heat-resistant conditions, and the color of Comparative Example 4 became slightly darker under light conditions. This indicates that an appropriate amount of sodium colanate and an appropriate amount of ceramide liposomes help improve the stability of the moisturizing essence.

[0172] The above embodiments are preferred implementation schemes of the present invention. In addition, the present invention can also be implemented in other ways. Any obvious replacement without departing from the concept of the present invention is within the scope of protection of the present invention.

Claims

1. A moisturizing and anti-aging nanoemulsion, characterized by: The invention comprises the following raw materials in mass percentage: 90-99% of an anti-aging component and 1-10% of a moisturizing component, wherein the anti-aging component comprises the following raw materials in mass percentage: 1-20% of a glycerol glucoside solution, 0.5-3% of nicotinamide adenine dinucleotide, 0.1-5% of sodium colanate, and the remainder of solvent; the moisturizing component comprises ceramide liposomes.

2. The moisturizing and anti-aging nanoemulsion according to claim 1, characterized in that: Each portion of the glycerol glucoside solution comprises the following raw materials in the following mass percentages: 45-55% of glycerol glucoside, 3-7% of 1,2-pentanediol, and 40-50% of water.

3. The moisturizing and anti-aging nanoemulsion according to claim 1, characterized in that: The ceramide liposome comprises the following raw materials in percentage by weight: ceramide NP 4-5%, ceramide EOP 0.000001-0.01%, ceramide NS / ceramide NG 0.01-0.2%, ceramide AS 0.01-0.2%, ceramide AP 0.000001-0.01%, hydrogenated lecithin 20-30%, and the balance glycerol.

4. The moisturizing and anti-aging nanoemulsion according to claim 1, characterized in that: The molecular weight of the sodium colanate is 20-5000 kDa.

5. The moisturizing and anti-aging nanoemulsion according to claim 1, characterized in that: The solvent is at least one of ionized water and alcohol solvents; the alcohol solvent is at least one of 1,3-propylene glycol, butylene glycol, dipropylene glycol, 1,2-hexanediol and glycerol.

6. A method for preparing the moisturizing and anti-aging nanoemulsion according to any one of claims 1 to 5, characterized in that: The steps include: (1) Preparing a moisturizing component: ceramide NP, ceramide EOP, ceramide NS / ceramide NG, ceramide AS, ceramide AP, hydrogenated lecithin, and glycerol were placed in an emulsifying pot, heated to 60-80°C, and homogenized at a speed of 15,000-28,000 r / min for 3-8 minutes. After homogenization, the mixture was transferred to a microfluidizer and homogenized at a pressure of 500-700 bar and a temperature of 40-80°C for 2-4 cycles to obtain a moisturizing component; (2) Preparing an anti-aging component: putting glycerol glucoside, nicotinamide adenine dinucleotide, sodium colanate and a solvent into a stirring pot, stirring and dissolving them uniformly, and then transferring them into an ultrasonic treatment pot, performing low-temperature ultrasonic treatment at a temperature of 25-40° C. for 10 min-30 min, wherein the ultrasonic frequency is 20 kHz-40 kHz, to obtain an anti-aging component; (3) The anti-aging component and the moisturizing component are mixed and stirred evenly, and transferred to a microfluidizer, and the mixture is homogenized at a pressure of 600-900 bar, a temperature of 40-80° C., and a cycle number of 3-6 times to obtain a moisturizing anti-aging nanoemulsion.

7. A moisturizing essence, characterized by: The invention comprises the moisturizing and anti-aging nanoemulsion according to any one of claims 1 to 5.

8. The moisturizing essence according to claim 7, characterized in that: The invention comprises the following raw materials in percentage by mass: 4-6% of moisturizing and anti-aging nanoemulsion, 2-6% of glycerol, 0.8-1.2% of glycerol polyether, 0.05-0.15% of xanthan gum, 0.1-0.3% of carbomer, 0.1-0.3% of arginine, 2-4% of butylene glycol, 0.2-0.6% of 1,2-hexanediol, 0.2-0.6% of p-hydroxyacetophenone, and the balance of water.

9. The moisturizing essence according to claim 8, characterized in that: The glycerol polyether is glycerol polyether-26.

10. A method for preparing the moisturizing essence according to claim 9, characterized in that: The steps include: (S1) adding a portion of water to a container, then adding a pre-mixed mixture of glycerol, glycerol polyether, xanthan gum, and carbomer, stirring, then heating to 80-90° C., and homogenizing until there are no particles, so that all the raw materials are evenly dispersed, to obtain phase A; (S2) adding arginine to a small amount of water and stirring to dissolve to obtain phase B; (S3) mixing p-hydroxyacetophenone, 1,2-hexanediol, and butanediol and heating to 60-80° C. to uniformly disperse the raw materials to obtain phase C; (S4) adding phase B and phase C to phase A cooled to 50-70°C, mixing uniformly, and obtaining a mixed solution; (S5) The mixed solution is cooled to below 40° C., and the moisturizing and anti-aging nanoemulsion is added and mixed evenly to obtain a moisturizing essence.

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