Composition with skin whitening and brightening effects and preparation method thereof

Abstract

The invention belongs to the technical field of functional food and health food, and particularly relates to a composition with skin whitening and brightening effects and a preparation method thereof. The composition comprises rice peptide, orange osmanthus pollen, white tomato powder, tremella polysaccharide, pomegranate concentrated powder and nicotinamide. Experimental results show that the composition reduces synthesis of melanin by inhibiting the activity of tyrosinase and achieves the effects of whitening and brightening the skin.

Description

Technical Field

[0001] The present invention belongs to the technical field of functional foods and health foods, and particularly relates to a composition with whitening and brightening effects and a preparation method thereof. Background Art

[0002] As the largest organ and the outermost barrier of the human body, the skin covers the whole body and can provide systemic protection to the body. This protection is of great significance for maintaining the body from being damaged by changes in external factors. With the increasingly harsh natural environment and the continuous increase of people's living pressure, various skin diseases such as dull and loose skin are becoming more and more common. Improving skin color has become an increasingly important need for modern humans, and the research and development of products with whitening effects have also attracted much attention.

[0003] For example, Chinese Patent CN111990558A discloses a whitening and antioxidant compound fruit drink with good absorption effect and a preparation method thereof, which includes active ingredients, beneficial ingredients and water. The active ingredients include rice peptides, etc., and the beneficial ingredients include tremella polysaccharide, pomegranate concentrate powder, red raspberry concentrate juice, etc. This patent discloses that ellagic acid in pomegranate concentrate powder can effectively inhibit the activity of tyrosinase, thereby blocking the generation of melanin after sun exposure. Red raspberries can achieve the purpose of beauty, skin care and prolonging life. However, this patent does not disclose the whitening effect experiment of the compound fruit drink, and the effect of inhibiting tyrosinase activity is uncertain. Summary of the Invention

[0004] Therefore, to solve the above technical problems, the present invention provides a composition with whitening and brightening effects and a preparation method thereof. This composition can effectively inhibit the activity of tyrosinase and achieve the effect of whitening and brightening the skin.

[0005] Therefore, the technical content of the present invention is as follows:

[0006] On the one hand, the present invention provides a composition with whitening and brightening effects, including rice peptides, osmanthus powder, white tomato powder, tremella polysaccharide, pomegranate concentrate powder and niacinamide.

[0007] Preferably, the composition with whitening and brightening effects provided by the present invention, by weight, includes 20-40 parts of rice peptides, 15-30 parts of osmanthus powder, 5-10 parts of white tomato powder, 3-8 parts of tremella polysaccharide, 1-6 parts of pomegranate concentrate powder, and 0.5-1.2 parts of niacinamide.

[0008] Among them, preferably, the preparation method of the osmanthus powder is: after mixing osmanthus flowers and water, ultrasonic extraction is carried out, separation is carried out, and the supernatant is dried to obtain.

[0009] Further preferably, in the method for preparing the osmanthus flower pollen, the conditions of ultrasonic treatment are as follows: the ultrasonic power is 150 - 200 W, the ultrasonic temperature is 30 - 50 °C, and the ultrasonic time is 60 - 80 min.

[0010] Further preferably, in the method for preparing the osmanthus flower pollen, the material - liquid ratio of osmanthus flower to water is 1:(30 - 50); more preferably, the material - liquid ratio of osmanthus flower to water is 1:40.

[0011] Further preferably, in the method for preparing the osmanthus flower pollen, the drying is spray drying, and the conditions are as follows: the inlet air temperature is 90 - 120 °C, and the outlet air temperature is 45 - 70 °C.

[0012] Preferably, the weight ratio of the osmanthus flower pollen to the white tomato powder is (1 - 6):1.

[0013] Preferably, the weight ratio of the rice peptide to the white tomato powder is (3 - 4):1.

[0014] Further preferably, the whitening and brightening composition provided by the present invention, by weight, comprises 30 parts of rice peptide, 25 parts of osmanthus flower pollen, 8 parts of white tomato powder, 6 parts of tremella polysaccharide, 4 parts of pomegranate concentrate powder, and 0.9 part of nicotinamide.

[0015] On the other hand, the present invention also provides a method for preparing the above - mentioned whitening and brightening composition, and the steps are as follows: weighing a quantitative amount of rice peptide, osmanthus flower pollen, white tomato powder, tremella polysaccharide, pomegranate concentrate powder, and nicotinamide, stirring and mixing them evenly, and then performing ultraviolet sterilization to obtain the composition.

[0016] Preferably, the stirring speed is 50 - 100 rpm, and the stirring time is 30 - 50 min.

[0017] Use of the composition and the composition obtained by the above - mentioned preparation method in the preparation of whitening and brightening products.

[0018] Compared with the prior art, the present invention has the following advantages:

[0019] 1. The present invention provides a composition with skin whitening and lightening effects, comprising rice peptide, sweet osmanthus flower pollen, white tomato powder, tremella polysaccharide, pomegranate concentrated powder and niacinamide, wherein white tomato is a tomato variety native to South America, rich in various lycopene and carotenoids and other biologically active ingredients, and existing studies have shown that supplementing white tomatoes is beneficial to anti-aging and reducing the risk of chronic diseases; tremella polysaccharide has a good antioxidant effect; niacinamide can inhibit the synthesis of melanin; sweet osmanthus contains flavonoids and phenolic substances, and has good anti-inflammatory, antioxidant and whitening effects; and the present invention provides a method for preparing sweet osmanthus flower pollen, which is prepared by ultrasonic extraction using sweet osmanthus flower as raw material and water as extraction solvent, and the above six raw materials have a synergistic effect in combination, and have a good effect of inhibiting tyrosinase activity, and have skin whitening and lightening effects.

[0020] 2. The preparation process of the osmanthus fragrans pollen provided by the present invention is simple, and the preparation process of the composition is also simple, and is suitable for industrial production. DETAILED DESCRIPTION

[0021] The following non-limiting examples can enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, which should also fall within the scope of the present invention.

[0022] When the embodiments give numerical ranges, it should be understood that, unless otherwise specified in the present invention, both endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used in the present invention have the same meanings as those generally understood by those of ordinary skill in the art to which the present invention belongs.

[0023] The present invention is further described below by way of specific examples. The various chemical reagents used in the examples of the present invention are obtained through conventional commercial channels unless otherwise specified. Products from different manufacturers have no significant effect on the effects.

[0024] Rice peptide was purchased from Hainan Yuanpeptide Biotechnology Co., Ltd. (average molecular weight <1000D); Tremella polysaccharide was purchased from Tremella polysaccharide from Shanxi Zhiyang Biotechnology Co., Ltd. (effective ingredient >98%); pomegranate concentrate powder was purchased from Lanzhou Waters Biotechnology Co., Ltd. (extraction ratio of 10:1); niacinamide was purchased from Zhengzhou Best Food Additive Co., Ltd. (content 99%); white tomato powder was purchased from Shanyi Food (Shanghai) Co., Ltd., with a specification of phytoene content ≥13 mg / g; acerola cherry concentrate powder was purchased from Baoji Liupanyun Biotechnology Co., Ltd. (specification of 10:1).

[0025] Preparation Example 1

[0026] This preparation example provides a kind of osmanthus flower pollen, and its preparation method is as follows: (1) Crush the osmanthus flower into powder, weigh 100 g of osmanthus flower powder, add 4000 mL of water to dissolve it, and obtain an osmanthus flower mixed solution; (2) Place the osmanthus flower mixed solution in an ultrasonic cleaner, and under the ultrasonic power of 180 W and the temperature of 40 °C, perform ultrasonic extraction for 70 min to obtain an osmanthus flower ultrasonic extraction solution; (3) Separate the solid and liquid of the osmanthus flower ultrasonic extraction solution, and the supernatant is the osmanthus flower extract; Concentrate the osmanthus flower extract and perform spray drying at the inlet air temperature of 100 °C and the outlet air temperature of 50 °C to obtain osmanthus flower powder.

[0027] Preparation Example 2

[0028] This preparation example provides a kind of osmanthus flower pollen, and its preparation method is as follows: (1) Crush the osmanthus flower into powder, weigh 100 g of osmanthus flower powder, add 3000 mL of water to dissolve it, and obtain an osmanthus flower mixed solution; (2) Place the osmanthus flower mixed solution in an ultrasonic cleaner, and under the ultrasonic power of 150 W and the temperature of 50 °C, perform ultrasonic extraction for 60 min to obtain an osmanthus flower ultrasonic extraction solution; (3) Separate the solid and liquid of the osmanthus flower ultrasonic extraction solution, and the supernatant is the osmanthus flower extract; Concentrate the osmanthus flower extract and perform spray drying at the inlet air temperature of 90 °C and the outlet air temperature of 45 °C to obtain osmanthus flower powder.

[0029] Preparation Example 3

[0030] This preparation example provides a kind of osmanthus flower pollen, and its preparation method is as follows: (1) Crush the osmanthus flower into powder, weigh 100 g of osmanthus flower powder, add 5000 mL of water to dissolve it, and obtain an osmanthus flower mixed solution; (2) Place the osmanthus flower mixed solution in an ultrasonic cleaner, and under the ultrasonic power of 200 W and the temperature of 30 °C, perform ultrasonic extraction for 80 min to obtain an osmanthus flower ultrasonic extraction solution; (3) Separate the solid and liquid of the osmanthus flower ultrasonic extraction solution, and the supernatant is the osmanthus flower extract; Concentrate the osmanthus flower extract and perform spray drying at the inlet air temperature of 120 °C and the outlet air temperature of 70 °C to obtain osmanthus flower powder.

[0031] Example 1

[0032] This example provides a composition with the efficacy of whitening and brightening the skin: including 30 g of rice peptide, 25 g of osmanthus flower powder (Preparation Example 1), 8 g of white tomato powder, 6 g of tremella polysaccharide, 4 g of pomegranate concentrate powder, and 0.9 g of niacinamide; Its preparation method is: Place the weighed raw materials in a roller mixer and stir at a speed of 80 rpm for 40 min, and obtain the composition after ultraviolet sterilization.

[0033] Example 2

[0034] This example provides a composition with whitening and brightening effects, which includes 20 g of rice peptides, 30 g of osmanthus flower powder (Preparation Example 2), 5 g of white tomato powder, 3 g of tremella polysaccharide, 6 g of pomegranate concentrate powder, and 0.5 g of niacinamide. The preparation method is as follows: Place the weighed raw materials in a drum mixer and stir at a speed of 50 rpm for 50 min, and obtain the composition after ultraviolet sterilization.

[0035] Example 3

[0036] This example provides a composition with whitening and brightening effects, which includes 40 g of rice peptides, 15 g of osmanthus flower powder (Preparation Example 3), 10 g of white tomato powder, 8 g of tremella polysaccharide, 1 g of pomegranate concentrate powder, and 1.2 g of niacinamide. The preparation method: Place the weighed raw materials in a drum mixer and stir at a speed of 100 rpm for 30 min, and obtain the composition after ultraviolet sterilization.

[0037] Comparative Example 1

[0038] This comparative example provides a composition with whitening and brightening effects. The only difference from Example 1 is that the osmanthus flower powder and white tomato powder are replaced with rice peptides, that is, it includes 63 g of rice peptides, 6 g of tremella polysaccharide, 4 g of pomegranate concentrate powder, and 0.9 g of niacinamide. The preparation method is the same as that of Example 1.

[0039] Comparative Example 2

[0040] This comparative example provides a composition with whitening and brightening effects. The only difference from Example 1 is that the rice peptides and white tomato powder are replaced with osmanthus flower powder, that is, it includes 63 g of osmanthus flower powder (Preparation Example 1), 6 g of tremella polysaccharide, 4 g of pomegranate concentrate powder, and 0.9 g of niacinamide. The preparation method is the same as that of Example 1.

[0041] Comparative Example 3

[0042] This comparative example provides a composition with whitening and brightening effects. The only difference from Example 1 is that the rice peptides and osmanthus flower powder are replaced with white tomato powder, that is, it includes 63 g of white tomato powder, 6 g of tremella polysaccharide, 4 g of pomegranate concentrate powder, and 0.9 g of niacinamide. The preparation method is the same as that of Example 1.

[0043] Comparative Example 4

[0044] This comparative example provides a composition with whitening and brightening effects. The only difference from Example 1 is that the white tomato powder is replaced with acerola cherry concentrate powder, that is, it includes 30 g of rice peptides, 25 g of osmanthus flower powder (Preparation Example 1), 8 g of acerola cherry concentrate powder, 6 g of tremella polysaccharide, 4 g of pomegranate concentrate powder, and 0.9 g of niacinamide; the preparation method is the same as that of Example 1.

[0045] Comparative Example 5

[0046] This comparative example provides a composition with whitening and brightening effects. The difference from Example 1 is that osmanthus fragrans powder is replaced with osmanthus fragrans extract, that is, it includes 30 g of rice peptide, 25 g of osmanthus fragrans extract, 8 g of white tomato powder, 6 g of tremella polysaccharide, 4 g of pomegranate concentrate powder, and 0.9 g of niacinamide. The preparation method is the same as that of Example 1.

[0047] Preparation method of osmanthus fragrans extract: (1) Crush osmanthus fragrans into powder, weigh 100 g of osmanthus fragrans powder, add 4000 mL of water to dissolve it to obtain an osmanthus fragrans mixed solution; (2) Place the osmanthus fragrans mixed solution in an ultrasonic cleaner, and under an ultrasonic power of 180 W and a temperature of 40 °C, ultrasonically extract for 70 min to obtain an osmanthus fragrans ultrasonic extraction solution; (3) Separate the solid and liquid of the osmanthus fragrans ultrasonic extraction solution, and the supernatant is the osmanthus fragrans extract; Concentrate the osmanthus fragrans extract and spray-dry it at an inlet air temperature of 100 °C and an outlet air temperature of 50 °C to obtain osmanthus fragrans extract.

[0048] Comparative Example 6

[0049] This comparative example provides a composition with whitening and brightening effects. The difference from Example 1 is only that the preparation and extraction method of osmanthus fragrans powder is different, specifically: (1) Crush osmanthus fragrans into powder, weigh 100 g of osmanthus fragrans powder, add 3000 mL of 40% ethanol solution to dissolve it to obtain an osmanthus fragrans mixed solution; (2) For the osmanthus fragrans mixed solution, the ratio of ultrasonic working time to ultrasonic gap time is 5:2, the ultrasonic power is 60 W, and under a temperature of 60 °C, ultrasonically extract for 15 min to obtain an osmanthus fragrans ultrasonic extraction solution; (3) Separate the solid and liquid of the osmanthus fragrans ultrasonic extraction solution, and the supernatant is the osmanthus fragrans extract; Concentrate and freeze-dry the osmanthus fragrans extract to obtain osmanthus fragrans powder.

[0050] Comparative Example 7

[0051] This comparative example provides a composition with whitening and brightening effects. The difference from Example 1 is only that the preparation and extraction method of osmanthus fragrans powder is different, specifically: Crush osmanthus fragrans into powder, weigh 100 g of osmanthus fragrans powder, place it in a round-bottom flask, add 4000 mL of pure water, and slowly heat it to a slightly boiling state with an electric heating mantle. After maintaining the slightly boiling state for 3 h, stop heating, cool, filter to collect the filtrate, and repeat the extraction once for the filter residue with 20 times the amount of pure water. Collect and combine the filtrates, and spray-dry to finally obtain osmanthus fragrans powder.

[0052] Experimental Example Determination of Viability, Melanin Content and Tyrosinase Activity of Mouse Melanoma Cells

[0053] Samples: Compositions obtained in Examples 1-3 and Comparative Examples 1-7

[0054] 1. Cell viability was determined by the CCK-8 kit method: After quickly thawing B16-F10 melanoma cells stored in liquid nitrogen, transfer them to a centrifuge tube, add 9 mL of sterile PBS solution, centrifuge at 1000 rpm for 5 min to remove the supernatant, resuspend with 5 mL of DMEM high-glucose medium containing 10% fetal bovine serum, and then transfer to a cell culture flask. Culture at 37 °C and 5% CO2 for 24 h, and passage until the state is stable, with a cell concentration of 5×10 6 cells / mL.

[0055] Inoculate B16-F10 cells at a cell density of 5×10 6 cells / mL into a 96-well plate. When the cells grow to about 90%, wash them once with HBSS, discard the HBSS, add them to DMEM high-glucose medium containing samples with different concentrations (0, 0.2, 0.4, 0.8 mg / mL), and culture in an incubator at 37 °C and 5% CO2 for 48 h. Then wash them once with HBSS, discard the HBSS, add 110 μL (containing 10 μL of CCK-8 reagent) of DMEM culture medium, culture for 1 h, and measure the absorbance at 450 nm. The formula for calculating cell survival rate is as follows: , where: A is the absorbance of the experimental group or the blank control group with a sample concentration of 0; B is the absorbance of the blank control group with a sample concentration of 0.

[0056] B16-F10 cells (mouse skin melanoma cells) were purchased from Wuhan Shang'en Biotechnology Co., Ltd.; DMEM (containing double antibiotics, without sodium pyruvate), product number 12100-10*500 mL was purchased from Beijing Solarbio Science & Technology Co., Ltd.; PBS was purchased from Shanghai Macklin Biochemical Co., Ltd., model number P787575; HBSS was purchased from Wuhan Sevier Biotechnology Co., Ltd., product number G4203-500 mL; CCK8 kit was purchased from Beyotime Biotechnology, product number C0038.

[0057] 2. Determination of melanin content by sodium hydroxide lysis method: Inoculate B16-F10 cells at a density of 5×10 6Inoculate the cell count of cell / mL into a 6-well plate. When the cells grow to about 90%, add 400 nmol / L melanin stimulator (α-MSH) to establish the model (except for the blank control group). After modeling, add the samples of Examples 1-3 and Comparative Examples 1-7 at the same concentration as the dosing groups; the group without adding any samples is used as the model control group; add 1 mmol / L arbutin solution as the positive control group. After culturing in a 37°C, 5% CO2 incubator for 48 h, wash once with HBSS, discard the HBSS, add 400 μL of trypsin diluted 4 times to digest for 3 min, transfer the cells to a 1.5 mL centrifuge tube, centrifuge at 1000 rpm for 5 min, and discard the supernatant. Add 300 μL of NaOH (1 mol / L) solution containing 10% dimethyl sulfoxide, incubate in a water bath at 60°C for 2 h, and measure the absorbance at 475 nm using an enzyme-linked immunosorbent assay (ELISA) reader. , where A is the absorbance of the blank control group, dosing group, positive control group, or model control group; B is the absorbance of the model control group.

[0058] B16-F10 cells (mouse skin melanoma cells) were purchased from Wuhan Shang'en Biotechnology Co., Ltd.; DMEM (containing double antibodies, without sodium pyruvate, product number 12100-10*500 mL), arbutin (product number IA0440-100 mg), dimethyl sulfoxide (cell culture grade, product number D8371-100 mL), trypsin (product number T8150) were purchased from Beijing Solarbio Science & Technology Co., Ltd.; HBSS was purchased from Wuhan Sevier Biotechnology Co., Ltd., product number G4203-500 mL; α-MSH was purchased from APExBIO Technology LLC, product number A1025.

[0059] 3. Detection of tyrosinase activity by the L-DOPA oxidation method: Seed B16-F10 cells at a cell density of 5×10 6 cell / mL into a 6-well plate. When the cells grow to about 90%, add α-MSH (400 nmol / L) to establish the model (except for the blank control group). After modeling, add the samples of Examples 1-3 and Comparative Examples 1-7 at the same concentration as the dosing groups; the group without adding any samples is used as the model control group; add arbutin solution (1 mmol / L) as the positive control group. After culturing in a 37°C, 5% CO2 incubator for 48 h, wash once with HBSS, discard the supernatant, add 300 μL of PBS solution containing 1% Triton X-100 to lyse the cells for 10 min, collect them in a 1.5 mL centrifuge tube, freeze at -80°C for 2 h, thaw at room temperature, centrifuge at 12000 rpm for 20 min, take 40 μL of the supernatant and mix it with 100 μL of L-DOPA (10 mmol / L), and react at 37°C in the dark for 30 min. Measure the absorbance at 475 nm using an enzyme-linked immunosorbent assay (ELISA) reader. , where A is the absorbance of the blank control group, the drug administration group, the positive control group, or the model control group, and B is the absorbance of the model control group.

[0060] B16-F10 cells (mouse skin melanoma cells) were purchased from Wuhan Shang'en Biotechnology Co., Ltd.; arbutin (product number IA0440-100mg), L-DOPA (product number ID0360-100mg), and Triton X-100 (product number T8200-100mL) were purchased from Beijing Solarbio Science & Technology Co., Ltd.; PBS was purchased from Shanghai Macklin Biochemical Co., Ltd., with the model number P787575; HBSS was purchased from Shanghai Yuchun Biotechnology Co., Ltd., with the product number G4203-500mL; α-MSH was purchased from APExBIO Technology LLC, with the product number A1025.

[0061] 4. Experimental results:

[0062] Data are expressed as mean ± standard deviation, and one-way ANOVA significance comparison was performed using Excel and SPSS 27 software.

[0063] 4.1 Viability of mouse melanoma cells

[0064] Table 1 Effects of Examples 1-3 and Comparative Examples 1-7 at different concentrations on the viability of B16F10 cells ( )

[0065]

[0066] (Note: represents P < 0.05 compared with the blank group, represents P < 0.01 compared with the blank group)

[0067] The effects of Examples 1-3 and Comparative Examples 1-7 on the viability of B16F10 cells are shown in Table 1.

[0068] When the concentrations of the compositions of Examples 1-3 and Comparative Examples 1-7 reached 0.8 mg / mL, the viability of each group of cells was significantly reduced compared with the blank control group. However, when the concentrations were 0.2 and 0.4 mg / mL, there was no significant difference in cell viability compared with the blank control group. Therefore, a concentration of 0.4 mg / mL was selected for subsequent cell experiments on melanin content and tyrosinase activity.

[0069] 4.2 Melanin content and tyrosinase activity

[0070] Tyrosinase is the most crucial enzyme in the process of melanin synthesis and plays a decisive role in melanin synthesis. The effects of different groups on the melanin content and tyrosinase activity of B16-F10 cells are shown in Table 2. Compared with the blank control group, the melanin content and tyrosinase activity in the model control group were significantly increased, indicating that the α-MSH-induced mouse skin melanoma cell model was successfully constructed.

[0071] Compared with the model control group, the melanin content and tyrosinase activity in Examples 1-3 were significantly decreased, indicating that the composition of the present invention has the effect of reducing melanin synthesis and inhibiting the production of melanin by α-MSH-induced mouse skin melanoma cells; compared with Example 1 and Comparative Examples 1-7, the melanin content and tyrosinase activity were significantly decreased, indicating that the types of raw materials selected in the present invention and the extraction method of osmanthus flower are the optimal combination, and by inhibiting the tyrosinase activity, the synthesis of melanin is significantly reduced; there was no significant difference in the melanin content and tyrosinase activity between Examples 1-3 and the positive control group, and the melanin content in Example 1 was lower than that in the positive control group, indicating that the combination ratio of Example 1 is the optimal combination.

[0072] Table 2 Effects of different groups on the melanin content and tyrosinase activity of α-MSH-induced B16-F10 cells ( )

[0073]

[0074] (Note: represents P<0.05 compared with the blank group, represents P<0.01 compared with the blank group; # represents P<0.05 compared with the model control group, ## represents P<0.01 compared with the model control group; △ represents P<0.05 compared with the positive control group, △△ represents P<0.01 compared with the positive control group; + represents P<0.05 compared with Example 1, ++ represents P<0.01 compared with Example 1.)

[0075] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than a limitation on the protection scope of the present invention. Any simple modification or equivalent replacement made by those of ordinary skill in the art to the technical solution of the present invention shall not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A composition having skin whitening and lightening effects, characterized in that: The invention comprises, by weight, 20-40 parts of rice peptide, 15-30 parts of osmanthus pollen, 5-10 parts of white tomato powder, 3-8 parts of tremella polysaccharide, 1-6 parts of red pomegranate concentrated powder and 0.5-1.2 parts of niacinamide; The preparation method of the osmanthus fragrans pollen is as follows: osmanthus fragrans flowers and water are mixed, ultrasonically extracted, separated, and the supernatant is dried to prepare the pollen.

2. The composition with skin whitening and lightening effects according to claim 1, characterized in that: In the method for preparing osmanthus fragrans pollen, the ultrasonic conditions are: ultrasonic power of 150-200W, ultrasonic temperature of 30-50°C, and ultrasonic time of 60-80min.

3. The composition with skin whitening and lightening effects according to claim 1, characterized in that: The solid-liquid ratio of the osmanthus flower to water is 1:(30-50).

4. The composition with skin whitening and lightening effects according to claim 1, characterized in that: The drying is spray drying, and the conditions are: the air inlet temperature is 90-120°C, and the air outlet temperature is 45-70°C.

5. The composition with skin whitening and lightening effects according to claim 1, characterized in that: The weight ratio of the osmanthus flower pollen to the white tomato powder is (1-6):

1.

6. The composition with skin whitening and lightening effects according to claim 1, characterized in that: The weight ratio of the rice peptide to the white tomato powder is (3-4):

1.

7. The composition with skin whitening and lightening effects according to claim 1, characterized in that: Calculated by weight, the invention comprises 30 parts of rice peptide, 25 parts of osmanthus pollen, 8 parts of white tomato powder, 6 parts of tremella polysaccharide, 4 parts of red pomegranate concentrated powder and 0.9 parts of nicotinamide.

8. A method for preparing the composition with skin whitening and lightening effects according to any one of claims 1 to 7, characterized in that: The method comprises the following steps: weighing a certain amount of rice peptide, osmanthus flower pollen, white tomato powder, tremella polysaccharide, pomegranate concentrated powder and nicotinamide, stirring and mixing them evenly, and performing ultraviolet sterilization to obtain a composition.

9. The preparation method according to claim 8, characterized in that: The stirring speed is 50-100rpm and the stirring time is 30-50min.

10. Use of the composition according to any one of claims 1 to 7 and the composition obtained by the preparation method according to any one of claims 8 to 9 in the preparation of skin whitening and lightening products.

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