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Reagents for detecting AChE (acetylcholinesterase) and preparation method thereof

A detection reagent and detection method technology, applied in the field of detection of acetylcholinesterase, can solve the problem of low sensitivity and achieve the effects of high detection sensitivity, low cost and good application potential

Inactive Publication Date: 2012-06-20
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The readout methods of the two methods are simple, but the sensitivity is still not high, and can only reach tens to hundreds of mU / mL. Therefore, the development of efficient biosensors for the detection of AChE still faces severe challenges

Method used

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  • Reagents for detecting AChE (acetylcholinesterase) and preparation method thereof
  • Reagents for detecting AChE (acetylcholinesterase) and preparation method thereof
  • Reagents for detecting AChE (acetylcholinesterase) and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0044] This embodiment provides a detection reagent for detecting AChE, including:

[0045] Deionized water;

[0046] Rhodamine B modified gold nanoparticles (RB-AuNPs), the concentration is 5nM;

[0047] Thioacetylcholine (ATC), the concentration is 20 μ M;

[0048] NaHCO 3 -NaOH mixture, so that the pH of the detection reagent is 10.

[0049] In this example, NaHCO 3 The addition of -NaOH mixture can prevent the aggregation of RB-AuNPs.

Embodiment 2

[0051]This embodiment provides the preparation method of the detection reagent of embodiment 1, comprising the following steps:

[0052] 1) Gold nanoparticles were synthesized by reducing chloroauric acid with sodium citrate to obtain an aqueous solution of gold nanoparticles with a concentration of 10nM;

[0053] 2) Dissolve Rhodamine B in deionized water to a concentration of 2mM, and wrap it in tinfoil to avoid light;

[0054] 3) Prepare NaHCO with a concentration of 5mM 3 -NaOH mixed solution, the pH value is 10;

[0055] 4) Take 5 mL of the newly prepared gold nanoparticle solution in step 1) and an equal volume of NaHCO 3 -NaOH solution mixed evenly;

[0056] 5) Take 6 μL of the rhodamine B solution obtained in step 2), and add it to the solution obtained in step 4), so that the final concentration of rhodamine B is about 1.2 μM. Wrapping and avoiding light to obtain a solution of rhodamine B-modified gold nanoparticles (RB-AuNPs);

[0057] 6) Take 1 mL of the solut...

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Abstract

The invention provides reagents for detecting AChE (acetylcholinesterase), comprising deionized water, rhodamine B-modified gold nanoparticles, thioacetylcholine, and material for preventing aggregation of gold nanoparticles. The invention further provides a preparation method of the reagents for detecting AChE.

Description

technical field [0001] The invention relates to a method for detecting acetylcholinesterase (acetylcholinesterase, AChE) by using surface-modified gold nanoparticles. Background technique [0002] Alzheimer's disease is a progressive and fatal neurodegenerative disease, which has become the fourth cause of death in the elderly after cardiovascular and cerebrovascular diseases, tumors and trauma. At present, the clinical diagnosis of Alzheimer's disease mainly relies on medical history, brain imaging and neuropsychological testing, but the sensitivity and specificity of diagnosis are not good, especially for patients in the early stage. The diagnosis and treatment of Alzheimer's disease still face great challenges. Cerebrospinal fluid examination is an important auxiliary diagnostic method after central nervous system damage. The condition of Alzheimer's disease is closely related to the level of some proteins in the cerebrospinal fluid of patients, such as Aβ, tau protein,...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/64G01N21/33G01N27/00G01N33/52
Inventor 蒋兴宇刘定斌王卓
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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