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Fast detecting reagent kit for toxicity of pesticide residue

A technology for detection kits and pesticide residues, which can be used in the determination/inspection of microorganisms, material analysis by observing the impact on chemical indicators, and analysis by making materials undergo chemical reactions. It can solve the problem of easy inactivation of enzymes and promote Difficulty, low sensitivity, etc.

Active Publication Date: 2009-08-12
广东恒度检测科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The application of these kits has solved the problem of detecting organophosphorus and carbamate pesticide residues in agricultural products to a certain extent. However, the sensitivity of these kits is not high, the enzyme is easily inactivated, and it is difficult to popularize.

Method used

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  • Fast detecting reagent kit for toxicity of pesticide residue
  • Fast detecting reagent kit for toxicity of pesticide residue
  • Fast detecting reagent kit for toxicity of pesticide residue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0162] A pesticide residue test kit, comprising

[0163] Phosphate buffer, pH8.0: Prepared with 0.1mol / L disodium hydrogen phosphate and sodium dihydrogen phosphate solution under the monitoring of pH meter.

[0164] Cholinesterase powder, wherein the mass ratio of acetylcholinesterase to butyrylcholinesterase is 20:80, when in use, dissolve 60 mg of cholinesterase powder in 8 ml of phosphate buffer solution with pH 8.0, and store at 4°C .

[0165] Substrate: When in use, 100 mg of thiobutyrylcholine iodide is dissolved in 10 ml of phosphate buffer with a pH of 8.0, and stored at 4°C.

[0166] Chromogenic solution: Dissolve 60 mg of DTNB in ​​10 ml of pH 8.0 phosphate buffer and store at 4°C.

Embodiment 2

[0168] A pesticide residue test kit, comprising

[0169] Phosphate buffer, pH7.5: Prepared with 0.1mol / L disodium hydrogen phosphate and sodium dihydrogen phosphate solution under the monitoring of pH meter.

[0170] Cholinesterase solution, wherein the mass ratio of acetylcholinesterase to butyrylcholinesterase is 30:70. The preparation method is as follows: 60 mg of cholinesterase powder is dissolved in 8 ml of pH 8.0 phosphate buffer, and stored at 4°C.

[0171] Substrate solution: when in use, take 100 mg of a mixture of thiobutyrylcholine iodide and thiobutyrylcholine bromide, dissolve it in 10 ml of phosphate buffer solution with a pH of 8.0, and store at 4°C.

[0172] Chromogenic solution: The preparation method is as follows: dissolve 60 mg of DTNB in ​​10 ml of pH 8.0 phosphate buffer and store at 4°C.

Embodiment 3

[0174] A pesticide residue test kit, comprising

[0175] Phosphate buffer, pH7.5: Prepared with 0.1mol / L disodium hydrogen phosphate and sodium dihydrogen phosphate solution under the monitoring of pH meter.

[0176] Cholinesterase powder, wherein the mass ratio of acetylcholinesterase to butyrylcholinesterase to mannitol is 15:80:5, when in use, dissolve 68.4 mg of cholinesterase powder in 8 ml of phosphoric acid at pH 8.0 buffer.

[0177] Substrate: When used, take 100 mg of a mixture of thiobutyrylcholine iodide, thiobutyrylcholine bromide and thiobutyrylcholine chloride in 10 ml of phosphate buffer at pH 8.0, at 4°C save.

[0178] Chromogenic solution: Dissolve 60 mg of DTNB in ​​10 ml of pH 8.0 phosphate buffer and store at 4°C.

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Abstract

The invention relates to a kit for quickly detecting toxicity of pesticide residue, in particular to a kit for quickly detecting the toxicity of pesticide residue containing a mixture of acetylcholinesterase and butyryl cholinesterase. The kit consists of choline esterase, a substrate, a color-developing agent 5,5'-dithio-2-2'-dinitrobenzoic acid (DTNB) and phosphate buffer solution, wherein the choline esterase is the mixture containing the acetylcholinesterase and the butyryl cholinesterase, and the substrate contains butyrylthiocholine iodide.

Description

technical field [0001] The invention relates to a rapid detection kit for pesticide residues, in particular to a rapid detection kit for pesticide residues containing a mixture of acetylcholinesterase and butyrylcholinesterase. Background technique [0002] In recent years, with the widespread application of pesticides in the cultivation of food, vegetables and fruits, accidents of pesticide poisoning injuries and fatalities have occurred frequently. In particular, with the increase of my country's export volume of agricultural products, due to excessive pesticide residues, the phenomenon of being rejected, detained, returned, destroyed, claimed and terminated contracts has occurred from time to time. Therefore, it is urgent to develop pesticide residue detection technology and vigorously strengthen the research on pesticide residue detection technology in agricultural products in my country, especially the rapid detection technology. [0003] At present, some kits with pes...

Claims

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Application Information

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IPC IPC(8): C12Q1/46G01N21/78
Inventor 余建飞
Owner 广东恒度检测科技有限公司
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