Quick detection card for pesticide residues based on naked eye visual colorimetric determination

A pesticide residue and rapid test card technology, which is applied in the field of rapid test card for pesticide residue detection, can solve the problems of low sensitivity, easy inactivation of enzymes, high subjectivity, etc., and achieve simple and rapid operation, high sensitivity and low cost Effect

Inactive Publication Date: 2015-11-11
FUDAN UNIV
View PDF6 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method solves the problem of rapid detection to a certain extent, it has problems such as low sensitivity and easy inactivation of enzymes.
Moreover, the

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quick detection card for pesticide residues based on naked eye visual colorimetric determination
  • Quick detection card for pesticide residues based on naked eye visual colorimetric determination
  • Quick detection card for pesticide residues based on naked eye visual colorimetric determination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] 1. Preparation of sample sheets

[0021] Prepare a 1mg / mL acetylcholinease solution, use a lettering machine to make a chromatographic paper disk with a diameter of 5mm, soak it in the enzyme solution to fully absorb it, take it out, and dry it at room temperature under nitrogen to make a sample sheet.

[0022] 2. Preparation of substrate sheet

[0023] The substrate source used in the present invention is analytically pure grade thioacetylcholine iodide, which is prepared as a 10 mg / mL solution for use (the solvent is deionized water). A printing paper disc with a diameter of 5 mm was made with a lettering machine. Pipette 10 μL of thioacetylcholine solution, carefully drop it into the center of the disk, and dry it at room temperature to obtain the substrate sheet.

[0024] 3. Preparation of test strips

[0025] Prepare 0.1% starch solution and 0.1mM iodine-0.1mM potassium iodide mixed solution for use. A chromatographic filter paper disc with a length of 3 cm and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of food safety, and particularly relates to a quick detection card for pesticide residues based on naked eye visual colorimetric determination. The quick detection card comprises fixed carrier membranes, a sample adding piece, a substrate piece and a detection strip, wherein acetylcholinesterase is immobilized on the sample adding piece; acetylthiocholine iodide is immobilized on the substrate piece; equal-interval colored tapes are arranged on the detection strip. The colored tapes on the detection strip can be faded through a product obtained after the acetylthiocholine iodide is hydrolyzed by the acetylcholinesterase, and certain pesticides have an inhibiting effect on the activity of the acetylcholinesterase. The quick detection card is based on the above principle, and pesticide residues can be semiquantitatively detected through counting the number of faded colored tapes on the detection strip; the quick detection card can be used for detecting common pesticide residues, is high in sensitivity, low in cost, and simple and quick to operate, improves the qualitative detection of the conventional quick detection card into semiquantitative detection, can be used for determining pesticide residues in samples such as vegetables, fruits and soil.

Description

technical field [0001] The invention belongs to the technical field of food safety, and in particular relates to a quick test card for detecting pesticide residues. Background technique [0002] my country is a large agricultural country with a wide variety of crops, including grains, vegetables, fruits and other crops. The invention and use of pesticides have greatly increased the yield of crops, especially organophosphorus pesticides, which are the most used pesticides in my country at this stage, and have greatly promoted the development of agriculture. However, due to excessive and improper use in recent years, the agricultural environment and food safety problems caused by pesticide residues and their pollutants have attracted more and more attention. [0003] Pesticide residues refer to the phenomenon that a part of pesticides directly or indirectly remain in grains, vegetables, fruits, livestock products, aquatic products, soil and water after pesticides are applied ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/78
Inventor 纪季李佳菁刘宝红杨芃原
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap