Quick detection card for pesticide residues based on naked eye visual colorimetric determination

Abstract

The invention belongs to the technical field of food safety, and particularly relates to a quick detection card for pesticide residues based on naked eye visual colorimetric determination. The quick detection card comprises fixed carrier membranes, a sample adding piece, a substrate piece and a detection strip, wherein acetylcholinesterase is immobilized on the sample adding piece; acetylthiocholine iodide is immobilized on the substrate piece; equal-interval colored tapes are arranged on the detection strip. The colored tapes on the detection strip can be faded through a product obtained after the acetylthiocholine iodide is hydrolyzed by the acetylcholinesterase, and certain pesticides have an inhibiting effect on the activity of the acetylcholinesterase. The quick detection card is based on the above principle, and pesticide residues can be semiquantitatively detected through counting the number of faded colored tapes on the detection strip; the quick detection card can be used for detecting common pesticide residues, is high in sensitivity, low in cost, and simple and quick to operate, improves the qualitative detection of the conventional quick detection card into semiquantitative detection, can be used for determining pesticide residues in samples such as vegetables, fruits and soil.

Description

technical field [0001] The invention belongs to the technical field of food safety, and in particular relates to a quick test card for detecting pesticide residues. Background technique [0002] my country is a large agricultural country with a wide variety of crops, including grains, vegetables, fruits and other crops. The invention and use of pesticides have greatly increased the yield of crops, especially organophosphorus pesticides, which are the most used pesticides in my country at this stage, and have greatly promoted the development of agriculture. However, due to excessive and improper use in recent years, the agricultural environment and food safety problems caused by pesticide residues and their pollutants have attracted more and more attention. [0003] Pesticide residues refer to the phenomenon that a part of pesticides directly or indirectly remain in grains, vegetables, fruits, livestock products, aquatic products, soil and water after pesticides are applied ...

AI Technical Summary

Problems solved by technology

Although this method solves the problem of rapid detection to a certain extent, it has problems such as low sensitivity and easy inactivation of enzymes.

Moreover, the results obtained only by naked eye observation are relatively subjective, and can only achieve qualitative detection results at most, and it is difficult to quantify

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