Color-developing method for rapid detection of residual organophosphorus and N-methyl carbamate pesticides

A technology of dithiodinitroformic acid and pesticides, which is applied in the fields of biotechnology and pesticides, and can solve problems such as inability to distinguish color differences, wrong judgments, and insignificant differences between negative and positive results.

Inactive Publication Date: 2012-05-16
上海福盈资产管理有限公司
View PDF3 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the judgment result of the speed tester method is more objective and accurate, it must rely on a spectrophotometer, and the naked eye cannot distinguish the difference in color development, so the promotio

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Color-developing method for rapid detection of residual organophosphorus and N-methyl carbamate pesticides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Experimental Materials

[0074] Chromogen A: saturated 2,6-dichloroindoxysodium (DCIP) solution (about 32 mM) at 4°C, and the solid powder of DCIP was dissolved in PBS (phosphate buffered saline).

[0075] Chromogenic agent B: a solution of dithiodinitroformic acid (DTNB) with a final concentration of 20 mM, and the solid powder of DTNB is dissolved in PBS.

[0076] Substrate: the final concentration is 40 mM thioacetylcholine (AchI) solution, and the solid powder of AchI is dissolved in PBS.

[0077] Mixing the developer: the developer A, the developer B and the substrate are mixed at a volume ratio of 1.2:1:1.

[0078] Acetylcholinesterase: The concentration is determined according to the activity of the enzyme, and the absorbance change value in 3 minutes is controlled above 0.3, and dissolved in PBS.

[0079] Experimental method steps:

[0080] 1. Take 50 μl of enzyme solution into EP tube, add 50 μl of pesticide solution or fruit and vegetable extract, and mix w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a rapid detection method for residual pesticides. The invention reveals an acetylcholinesterase-based color-developing method for the first time, and in the method, a special color-developing agent is adopted, so that negative and positive color-developing results are extremely obvious, and can be directly judged by naked eyes without using a spectrophotometer, therefore, the method has a good application prospect in the field of rapid detection on residual pesticides.

Description

technical field [0001] The invention belongs to the fields of biotechnology and pesticide science; more specifically, the invention relates to a rapid detection method for pesticide residues. Background technique [0002] Food safety, especially the safety of agricultural products has become the focus of attention. The problem of excessive pesticide residues in agricultural products has affected the safety of people's dining tables, thereby affecting people's health. Therefore, the problem of residues of agricultural products has attracted the attention of regulatory authorities at all levels, and testing stations have been established to detect and control them. [0003] At present, the detection methods of pesticide residues mainly include gas chromatography and rapid detection of pesticide residues. Although gas chromatography can be used for qualitative and quantitative detection of pesticide residues, due to methodological limitations, it is not suitable for a large n...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/78
Inventor 王慧吴松洁李井泉相丽王子良郝淼
Owner 上海福盈资产管理有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap