Color-developing method for rapid detection of residual organophosphorus and N-methyl carbamate pesticides
A technology of dithiodinitroformic acid and pesticides, which is applied in the fields of biotechnology and pesticides, and can solve problems such as inability to distinguish color differences, wrong judgments, and insignificant differences between negative and positive results.
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[0073] Experimental Materials
[0074] Chromogen A: saturated 2,6-dichloroindoxysodium (DCIP) solution (about 32 mM) at 4°C, and the solid powder of DCIP was dissolved in PBS (phosphate buffered saline).
[0075] Chromogenic agent B: a solution of dithiodinitroformic acid (DTNB) with a final concentration of 20 mM, and the solid powder of DTNB is dissolved in PBS.
[0076] Substrate: the final concentration is 40 mM thioacetylcholine (AchI) solution, and the solid powder of AchI is dissolved in PBS.
[0077] Mixing the developer: the developer A, the developer B and the substrate are mixed at a volume ratio of 1.2:1:1.
[0078] Acetylcholinesterase: The concentration is determined according to the activity of the enzyme, and the absorbance change value in 3 minutes is controlled above 0.3, and dissolved in PBS.
[0079] Experimental method steps:
[0080] 1. Take 50 μl of enzyme solution into EP tube, add 50 μl of pesticide solution or fruit and vegetable extract, and mix w...
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