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Color-developing method for rapid detection of residual organophosphorus and N-methyl carbamate pesticides

A detection method, the technology of dithiodinitroformic acid, is applied in the fields of biotechnology and pesticides, and can solve the problems of wrong judgment, inability to distinguish the difference in color development, and inconspicuous difference between negative and positive results.

Inactive Publication Date: 2013-07-10
上海福盈资产管理有限公司
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Problems solved by technology

Although the judgment result of the speed tester method is more objective and accurate, it must rely on a spectrophotometer, and the naked eye cannot distinguish the difference in color development, so the promotion of this method has certain limitations
Although the quick test card method is easy to operate, it is easy to cause subjective misjudgment because the difference between negative and positive results is not obvious.

Method used

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  • Color-developing method for rapid detection of residual organophosphorus and N-methyl carbamate pesticides

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Experimental program
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Effect test

Embodiment 1

[0073] Experimental Materials

[0074] Chromogen A: saturated 2,6-dichloroindoxysodium (DCIP) solution (about 32 mM) at 4°C, and the solid powder of DCIP was dissolved in PBS (phosphate buffered saline).

[0075] Chromogenic agent B: a solution of dithiodinitroformic acid (DTNB) with a final concentration of 20 mM, and the solid powder of DTNB is dissolved in PBS.

[0076] Substrate: the final concentration is 40 mM thioacetylcholine (AchI) solution, and the solid powder of AchI is dissolved in PBS.

[0077] Mixing the developer: the developer A, the developer B and the substrate are mixed at a volume ratio of 1.2:1:1.

[0078] Acetylcholinesterase: The concentration is determined according to the activity of the enzyme, and the absorbance change value in 3 minutes is controlled above 0.3, and dissolved in PBS.

[0079] Experimental method steps:

[0080] 1. Take 50 μl of enzyme solution into EP tube, add 50 μl of pesticide solution or fruit and vegetable extract, and mix w...

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Abstract

The invention relates to a rapid detection method for residual pesticides. The invention reveals an acetylcholinesterase-based color-developing method for the first time, and in the method, a special color-developing agent is adopted, so that negative and positive color-developing results are extremely obvious, and can be directly judged by naked eyes without using a spectrophotometer, therefore, the method has a good application prospect in the field of rapid detection on residual pesticides.

Description

technical field [0001] The invention belongs to the fields of biotechnology and pesticide science; more specifically, the invention relates to a rapid detection method for pesticide residues. Background technique [0002] Food safety, especially the safety of agricultural products has become the focus of attention. The problem of excessive pesticide residues in agricultural products has affected the safety of people's dining tables, thereby affecting people's health. Therefore, the problem of residues of agricultural products has attracted the attention of regulatory authorities at all levels, and testing stations have been established to detect and control them. [0003] At present, the detection methods of pesticide residues mainly include gas chromatography and rapid detection of pesticide residues. Although gas chromatography can be used for qualitative and quantitative detection of pesticide residues, due to methodological limitations, it is not suitable for a large n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 王慧吴松洁李井泉相丽王子良郝淼
Owner 上海福盈资产管理有限公司
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