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Magnetic electrode based on Fe3O4-AuNPs magnetic composite nanoparticle immobilized acetylcholinesterase and preparation and application thereof

A technology of acetylcholinesterase and magnetic composite nanometer, which is applied in the field of biosensors to achieve the effects of good electrochemical performance, ease of use, and good electrical conductivity

Active Publication Date: 2013-10-09
SHANDONG YANGCHENG BIOLOGY TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, with the development of composite nanomaterials, many composite materials of magnetic nanoparticles and other materials have emerged, such as Fe 3 o 4 AuNPs, Fe 3 o 4 PdNPs, Fe 3 o 4 C. Fe 3 o 4 / graphene and Fe 3 o 4 / SiO 2 / Bi 2 WO 6 、TiO 2 / SiO 2 Fe 3 o 4 However, there has been no report on the construction of enzyme biosensors for the detection of pesticide residues related to magnetic nanoparticle composites.

Method used

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  • Magnetic electrode based on Fe3O4-AuNPs magnetic composite nanoparticle immobilized acetylcholinesterase and preparation and application thereof
  • Magnetic electrode based on Fe3O4-AuNPs magnetic composite nanoparticle immobilized acetylcholinesterase and preparation and application thereof
  • Magnetic electrode based on Fe3O4-AuNPs magnetic composite nanoparticle immobilized acetylcholinesterase and preparation and application thereof

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Embodiment 1

[0023] Fe is removed by magnetic force 3 O 4 -AuNPs magnetic nanoparticles were modified on the magnetic glassy carbon electrode, and acetylcholinesterase was immobilized on it as a carrier. Its preparation process is as follows:

[0024] 1. Preparation of Magnetic Nanoparticles

[0025] (1) Fe 3 O 4 Preparation of nanoparticles

[0026] Preparation of Fe by chemical co-precipitation method 3 O 4 Nanoparticles. First, take 15-30 mL of secondary water and put it into a beaker to pass nitrogen and deoxidize, and add 4-6 g FeCl to the beaker. 3 ·6H 2 O, 3~5g FeCl 2 ·4H 2O, and 0.5-1 mL of HCl were dissolved in the deoxygenated secondary water, and then added dropwise to 250 mL of 1.5 mol / L NaOH solution at 80 °C with constant stirring, after aging for 1 h, washed with secondary water, the magnet separation, and finally the suspension was dispersed in 100 mL of secondary water for use.

[0027] (2) Fe 3 O 4 - Preparation of AuNPs

[0028] will Fe 3 O 4 After the ...

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Abstract

The invention discloses a magnetic electrode based on Fe3O4-AuNPs magnetic composite nanoparticle immobilized acetylcholinesterase, belonging to the technical field of biosensors. Fe3O4-AuNPs magnetic composite nanoparticles are modified on a magnetic glassy carbon electrode through a magnetic force, and the magnetic glassy carbon electrode is used as a carrier to immobilize the acetylcholinesterase. A preparation method of the magnetic electrode comprises the following steps: firstly, preparing Fe3O4-AuNPs, then, preparing the magnetic glassy carbon electrode, dripping and coating 0.10 g / L CNTs (Carbon Nanotubes) turbid liquid, airing, then, putting the MGCE (Magnetic Glassy Carbon Electrode) in Fe3O4-AuNPs turbid liquid, adsorbing, washing, airing, and dripping and coating glutaraldehyde and AChE. The electrode disclosed by the invention has good conductivity and electrochemical properties.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and relates to a Fe-based 3 O 4 -AuNPs magnetic composite nanoparticle-modified magnetic enzyme biosensor, preparation method and application thereof. Background technique [0002] Magnetic nanoparticles have always been a hot spot in analytical chemistry and environmental science research due to their unique structural and magnetic properties. Its large specific surface area and superparamagnetic properties make it an ideal carrier for immobilizing biomolecules. In recent years, with the development of composite nanomaterials, many composite materials of magnetic nanoparticles and other materials have emerged, such as Fe 3 O 4 AuNPs, Fe 3 O 4 PdNPs, Fe 3 O 4 C. Fe 3 O 4 / graphene and Fe 3 O 4 / SiO 2 / Bi 2 WO 6 , TiO 2 / SiO 2 Fe 3 O 4 etc., but there has been no report on the construction of an enzymatic biosensor for pesticide residue detection. AuNPs have good electrocata...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 康天放薛瑞张金果
Owner SHANDONG YANGCHENG BIOLOGY TECH CO LTD
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