Quick detection paper card for pesticide residue in vegetable and preparation method therof

A technology of pesticide residues and quick test cards, which is applied in the fields of biological testing, material inspection products, and analysis by making materials undergo chemical reactions.

Inactive Publication Date: 2006-02-15
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this quick test card must be equipped with a pesticide residue quick tester to detect it, and this quick test card can only detect organophosphorus pesticides

Method used

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  • Quick detection paper card for pesticide residue in vegetable and preparation method therof
  • Quick detection paper card for pesticide residue in vegetable and preparation method therof
  • Quick detection paper card for pesticide residue in vegetable and preparation method therof

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Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1, the screening of enzyme source

[0032] Using horse serum, human serum, bovine liver, bee head, housefly head and other animal organs or serum as materials, extract and screen the highly sensitive enzyme source-acetylcholinesterase according to conventional methods. Mash frozen horse serum, human serum, bovine liver, bee brain, and housefly brain respectively, add distilled water at a ratio of 1:9 (W / V), homogenate for 3 minutes, and centrifuge at 2000-3000r / min for 10 minutes , the supernatant was filtered through filter paper, freeze-dried, divided into equipment, and stored at -20°C, which was the enzyme source.

[0033] 1) Reagent preparation:

[0034] Enzyme solution preparation: Dilute freeze-dried horse serum enzyme powder, human serum enzyme powder, bovine liver enzyme powder, honeybee brain enzyme powder, and housefly brain enzyme powder with PH8., 0.1M phosphate buffer solution to prepare 0.75 units .

[0035] Pesticide concentration: 0.8ppm f...

Embodiment 2

[0043] Embodiment 2: the selection of enzyme concentration

[0044] 1) Enzyme preparation: Dilute the freeze-dried human serum enzyme powder of the present invention with pH 8.0 and 0.1M phosphate buffer to 0.5, 0.75, 1.0, 1.25, and 1.5 units respectively. Add one drop (about 30 μl) onto a qualitative filter paper with a diameter of 1.0 cm to make an enzyme tablet.

[0045] 2) Substrate: Mix 0.0448g potassium ferricyanide (red blood salt) and 0.0715g potassium ferrocyanide (yellow blood salt), add 100ml distilled water (PH5.5) after mixing, add one drop (about 30μl) in side becomes 1.0cm 2 On the qualitative filter paper, add dropwise 30μl indole acetate 180mg / 10ml acetone solution to make a substrate sheet.

[0046] 3) The concentration of methamidophos is 0.1 mg / μl.

[0047] 4) Use distilled water for comparison

[0048] The results of the reaction of five kinds of enzyme concentration enzyme tablets with the pesticide methamidophos are shown in Table 3

[0049] ...

Embodiment 3

[0056] Embodiment 3: the selection of optimal substrate concentration

[0057] 1) Preparation of substrate sheet: Mix 0.0448g potassium ferricyanide (red blood salt) and 0.0715g potassium ferrocyanide (yellow blood salt) into 100ml distilled water, take a drop of the mixture (about 30μl pH5.5) first Add dropwise on 3 pieces with a side length of 1.0em 2 Qualitative filter paper, and then put a drop (about 30μl) of indole acetate 180mg / 10ml acetone solution, indole acetate 270mg / 10ml acetone solution, indole acetate 360mg / 10ml acetone solution on three pieces of qualitative filter paper On the surface, dry at room temperature for use as a substrate sheet.

[0058] 2) Preparation of enzyme tablets: Dilute the enzyme powder extracted from the frozen human serum of the present invention with pH 8.0 and 0.1M phosphate buffer to 0.5 and 0.75 units respectively. Add it dropwise on a qualitative filter paper with a diameter of 1.0 cm to make an enzyme tablet.

[0059] The results o...

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Abstract

The present invention provides a quick reflection test card and making method of pesticide residue, which can adapt the acetylcholinesterase with extracting the serum of human as enzyme piece and measure the sensibility of organophosphorus and carbamate pesticide ranged from 0.001 to 1.2mg/kg, wherein the quick reflection test card is a rather sensitive enzyme source. This invention discloses a good heat-stability enzyme source, which lasts 7-8 hours by adding gelatin stabler or trehalose in more or less 90 deg.c circumstances.

Description

technical field [0001] The invention belongs to the analysis of pesticide residues, in particular, the invention relates to a rapid detection paper card for pesticide residues in vegetables and a preparation method thereof Background technique [0002] my country is a large agricultural country, with abundant crops. In addition to food crops, oil crops, fiber crops, sugar crops, melons and fruits, and medicinal tobacco, there are also large areas of fruits, vegetables, pastures, and forests. Therefore, China is also a big country in the application of pesticides. The invention and use of pesticides have undoubtedly greatly improved agricultural productivity, but the annual spraying of chemical pesticides to prevent and control pests and diseases is still an indispensable and necessary measure. With the improvement of people's quality of life, the demand for safe, nutritious, and pollution-free vegetables and fruits is becoming stronger and stronger. At present, the amount o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/52
Inventor 吴虹戴小杰
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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