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Pomfret sperm cryopreservation method

A cryopreservation and preservation method technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of silver pomfret resource decline, affect the success of artificial reproduction technology and fertilization rate, and achieve the effect of improving fertilization rate

Inactive Publication Date: 2011-10-19
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

However, due to overfishing and environmental pollution, the resources of silver pomfret are seriously declining and cannot meet the needs of consumers. It is the best choice to carry out research on artificial breeding technology of silver pomfret and provide high-quality seedlings
From the current point of view, the artificial breeding of silver pomfret adopts artificial insemination at sea, which requires simultaneous maturation of male and female. Under natural conditions, the development of male silver pomfret is faster than that of female fish, which will inevitably affect the success of artificial breeding technology and the fertilization rate.

Method used

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Embodiment Construction

[0017] The present invention will be further described below in conjunction with specific implementation method; It should be emphasized that: for those of ordinary skill in the art, can be improved or changed according to the above description, and all these improvements and changes should belong to the appended claims of the present invention protected range.

[0018] A silver pomfret sperm cryopreservation method, the steps are:

[0019] (1) Semen collection:

[0020] For male broodstock with mature selective gland development, the semen is obtained by abdominal extrusion method, and the semen is collected in a 5ml centrifuge tube. The semen with an activity rate of more than 85% is used for sperm freezing, and the semen is stored on ice after collection;

[0021] (2) Base fluid selection:

[0022] Base liquid selection: preparation method: NaCl7.5g / L, NaHCO 3 0.25g / L, KCl0.38g / L, CaCl 2 2H 2 O0.18g / L, MgCl 2 0.22g / L, NaH 2 PO 4 0.22g / L, D-glucose 1g / L, adjust pH to...

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Abstract

The invention relates to a pomfret sperm cryopreservation method including the steps as follows: selecting sexually matured parental pomfrets to gather fine-quality sperm; selecting suitable base liquid and anti-freezing liquid; mixing the sperm with the anti-freezing liquid by the volume ratio of 1:1 and then preserving the mixture on ice for 4-5 min; after reducing the temperature to minus 150 DEG C according to a three-step temperature reduction method, staying for 5 min; directly placing the mixture in liquid nitrogen for preservation; in defreezing, staying a freeze pipe in liquid nitrogen steam for 2 min, rapidly placing the freeze pipe in water bath of 28 DEG C to defreeze for 90 s and then placing the freeze pipe at room temperature to dissolve completely; and activating the defrozen sperm with sea water, wherein the rapid swimming time of the sperm is 57.41 s averagely, and the sperm viability (percent of the sperms moving rapidly accounting for all sperms) is 83.62% averagely. The fertilization rate of the pomfrets is greatly enhanced.

Description

technical field [0001] The invention belongs to the technical field of sperm freezing, and in particular relates to a method for cryopreserving silver pomfret sperm. Background technique [0002] Silver pomfret belongs to Perciformes, Pomfret suborder, Pomfretaceae, Pomfret genus, the meat is white, tender, less spines, rich in protein, fat, calcium, phosphorus, iron, sodium, potassium, vitamin A, vitamin B1, vitamin B2, Nutrients such as niacin can strengthen the body and prevent adult diseases such as myocardial infarction and cerebral thrombosis through regular consumption. However, due to overfishing and environmental pollution, the resources of silver pomfret are severely declining, which cannot meet the needs of consumers. It is the best choice to carry out research on artificial breeding technology of silver pomfret and provide high-quality seed. From the present point of view, the artificial breeding of silver pomfret adopts artificial insemination at sea, which req...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 崔青曼袁春营乔秀亭徐海龙
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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