Cell microinjection instrument

A technique of injecting instruments and cells, applied in the fields of biochemical instruments, enzymology/microbiology devices, biochemical equipment and methods, etc.
CN1763170AInactive Publication Date: 2006-04-26章维一 +2
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
章维一
Publication Date
2006-04-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The microscopic cell injecting instrument includes cell conveyer, cell injector, cell holder and digital control and drive system. In cell injection, cell is conveyed automatically with the cell conveyer and in microfluid conveying mode to the cell injector, and the outlet of the cell holder. During cell injection, all the cell conveyance, holding, cell membrane puncture with the injection needle, intracellular medicine injection, the withdrawn of injection needle, cell release and other microscopic operation steps are computer digital controlled in the same computer operating interface. The present invention has simple operation, high successful injection rate, and automatic separation and counting of injected and un-injected cells.
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Description

Technical field

[0001] The invention relates to a cell microinjection apparatus, which includes a cell transporter, a cell injector, a cell holder and a digital control driving system, which can realize digital microinjection of cells. Background technique

[0002] In the current prior art, cell microinjection mainly adopts a manual microinjection method and an active automatic cell injection method. The manual microinjection method takes the Nikon NT-88NE manual micromanipulator as an example. The operator observes visual information such as the enlarged image, pose, and distance between the injection tool and the cell through the microscope with the naked eye. Two sets of manual mechanical coarse adjustment and manual hydraulic fine adjustment mechanisms are installed on the inverted microscope to control the suction needle and injection respectively to find, locate, hold and inject cells. The injection process strongly depends on various factors of the operator itself, s...

Claims

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