Cell microinjection instrument

A technique of injecting instruments and cells, applied in the fields of biochemical instruments, enzymology/microbiology devices, biochemical equipment and methods, etc.

Inactive Publication Date: 2006-04-26
章维一 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of developing automated cell injection system and cell injection robot, the implementation of technological innovation in control and operation is limited by the development of many technologies such as micro-mechanical electronic technology, computer control and image recognition technology, and optical microscopy technology. This technology is still in experimental development. stage

Method used

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  • Cell microinjection instrument
  • Cell microinjection instrument
  • Cell microinjection instrument

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Experimental program
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Embodiment Construction

[0009] look first figure 1 , the experimental device consists of a cell transporter 1 and a transport micropipe 2 to form a cell transporter, a suction needle 4, a pressure device capable of generating positive and negative pressure, a suction device 5, and a cell syringe 18 for injecting medicinal liquid. A pressure sensor is installed in the 5, and the pressure sensor is connected with the computer 11 controlling the cell transporter through a control circuit. The micro-channel 2 is bent by 165° and installed on the cell transporter 1, and the cell transporter 1 is installed on the three-dimensional adjuster 12 capable of adjusting the three-dimensional direction. The holding needle 4 is bent at 135°, the cell holder 5 is installed on the right trimmer 6 with a 5-dimensional adjustment function at 45° to the horizontal plane, the three-dimensional adjuster 12 and the left trimmer 6 are all installed on the inverted microscope 7 on the stand. A cell injector 18 is installed...

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Abstract

The microscopic cell injecting instrument includes cell conveyer, cell injector, cell holder and digital control and drive system. In cell injection, cell is conveyed automatically with the cell conveyer and in microfluid conveying mode to the cell injector, and the outlet of the cell holder. During cell injection, all the cell conveyance, holding, cell membrane puncture with the injection needle, intracellular medicine injection, the withdrawn of injection needle, cell release and other microscopic operation steps are computer digital controlled in the same computer operating interface. The present invention has simple operation, high successful injection rate, and automatic separation and counting of injected and un-injected cells.

Description

Technical field [0001] The invention relates to a cell microinjection apparatus, which includes a cell transporter, a cell injector, a cell holder and a digital control driving system, which can realize digital microinjection of cells. Background technique [0002] In the current prior art, cell microinjection mainly adopts a manual microinjection method and an active automatic cell injection method. The manual microinjection method takes the Nikon NT-88NE manual micromanipulator as an example. The operator observes visual information such as the enlarged image, pose, and distance between the injection tool and the cell through the microscope with the naked eye. Two sets of manual mechanical coarse adjustment and manual hydraulic fine adjustment mechanisms are installed on the inverted microscope to control the suction needle and injection respectively to find, locate, hold and inject cells. The injection process strongly depends on various factors of the operator itself, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
Inventor 章维一侯丽雅刘天军
Owner 章维一
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