Cell model having undergone vicious transformation

A cell and malignant technology, applied in the field of cell biology, can solve the problems of unfavorable large amount of chemicals, insufficient correlation between animal experiments and human body, and large investment.

Active Publication Date: 2016-07-27
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditional food toxicology is mainly based on animal experiments, and its limitations are mainly manifested in: 1) The correlation between animal experiments and human body is not sure enough; 2) The conclusions of animal experiments based on high-dose intake deviate from reality (low-dose long-term exposure) Very large; 3) The observation endpoint is clinical features or pathological changes, and early changes in the body cannot be detected; 4) It is difficult to explain the toxicity mechanism from the perspective of multiple receptors / information transmission pathways and multiple metabolic networks; 5) Animal experiments are time-consuming and expensive , which is not conducive to high-throughput detection of the toxicity of a large number of chemicals

Method used

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  • Cell model having undergone vicious transformation
  • Cell model having undergone vicious transformation
  • Cell model having undergone vicious transformation

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Experimental program
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Effect test

Embodiment 1

[0056] Embodiment 1, screening the cells of the present invention

[0057] Take the normal human liver cell line HL-7702 (ATCC), add different concentrations of benzo(a)pyrene (0.01nM, 1nM, 100nM) to the medium for long-term culture, and observe the cells under the microscope every 2-3 days Growth status, including cell shape, survival rate, growth rate, etc. In the screen, it was found that sustained stimulation with 100 nM benzo(a)pyrene resulted in a decrease in cell viability in the short term. Among them, there was a cell line with significant morphological changes, but the cell viability did not decrease, and it also showed stronger growth ability. This phenotypic change still persisted after the withdrawal of benzo(a)pyrene, indicating that the cell Irreversible changes have taken place and transformed into a new cell type with stable traits, which is the cell line obtained by screening in the present invention. The inventor named it "human normal liver cell benzopyre...

Embodiment 2

[0059] Embodiment 2, the morphological change of the cell obtained by screening

[0060] Comparing the cell lines obtained by the aforementioned screening with normal liver cells not treated with benzo(a)pyrene, it was found that the morphology of the cell lines of the present invention has changed significantly. Such as figure 1 As shown in Fig. 1, the morphology changed from round to regular and spindle-shaped cells. This morphological change indicates that the cell has exhibited different cellular characteristics from the source cell, and this is caused by the continuous stimulation of benzo(a)pyrene.

[0061] The above results suggest that the cell line of the present invention can be used as a new cell model for studying how benzo(a)pyrene affects cytoskeleton and morphology.

Embodiment 3

[0062] Embodiment 3, the growth situation of the cell obtained by screening

[0063] The growth of the cell lines obtained from the aforementioned screening was compared with normal liver cells not treated with benzo(a)pyrene. The result is as figure 2 , it was found that the growth rate of the cell line of the present invention was significantly increased, indicating that compared with the source cells, the cell line obtained by screening had abnormally fast growth ability, indicating that the continuous stimulation of benzo(a)pyrene changed the division and proliferation ability of cells.

[0064] The above results show that the cell line screened by the present invention can be used as a new cell model for studying how benzo(a)pyrene affects cell division and proliferation.

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Abstract

The invention relates to a cell model having undergone vicious transformation. A novel cell line having undergone vicious transformation is developed on the basis of human-derived normal cell for the first time in the invention. The cell line is screened out after long-term stimulation by low-concentration benzopyrene; and identification results show that the cell model has undergone vicious transformation. The cell line can be used as a cell model for research on pathogenic effect of benzopyrene or screening of drugs capable of inhibiting vicious transformation of cells.

Description

technical field [0001] The present invention relates to the field of cell biology, more specifically, the present invention relates to a cell model undergoing malignant transformation. Background technique [0002] With the development of science and technology, new chemical substances are increasing day by day, and the traditional toxicological evaluation methods are facing new challenges. Traditional food toxicology is mainly based on animal experiments, and its limitations are mainly manifested in: 1) The correlation between animal experiments and human body is not sure enough; 2) The conclusions of animal experiments based on high-dose intake deviate from reality (low-dose long-term exposure) Very large; 3) The observation endpoint is clinical features or pathological changes, and early changes in the body cannot be detected; 4) It is difficult to explain the toxicity mechanism from the perspective of multiple receptors / information transmission pathways and multiple meta...

Claims

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Application Information

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IPC IPC(8): C12N5/09C12N5/071C12Q1/02
Inventor 王慧巴乾李井泉
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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