The invention belongs to the technical field of bioengineering, and particularly relates to an A2aR-His6-G alpha 16-HEK
cell strain capable of stably expressing a human
adenosine A2a
receptor (A2aR). According to the invention, the Galpha16
protein and the A2aR which are determined by screening are sequentially expressed in HEK-FlpIn cells, and the A2aR-His6-Galpha16-HEK
cell strain capable of stably expressing the human A2aR is obtained by screening; a
cell evaluation model suitable for
calcium signal detection is constructed, and the
cell evaluation model can be used for screening and detecting A2aR-targeting agonists and antagonists; the kit has the characteristics of more reliable detection result, higher sensitivity, larger screening flux, short experimental period, simplicity and convenience in operation and the like, can greatly improve the screening and evaluation efficiency of A2aR-targeting agonists and antagonists, and meanwhile, has a significant meaning for A2aR-related diseases. Candidate
drug molecules are provided for treatment of diseases such as
motion control, neurodegenerative diseases, tumor immunoregulation, anti-depression effect, heart diseases, nerve regeneration and immunoregulation, the problem that at present, A2aR target-based
drug screening does not have a high-
throughput detection method based on
calcium signal detection is solved, and the method has obvious practical application value.