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Constructing method of model of lacking of Langerhans cells of small rat skin

A construction method and mouse technology, which can be used in pharmaceutical formulations, preparations for in vivo experiments, and medical preparations containing active ingredients, etc., can solve problems such as reducing the density of epidermal Langerhans cells, achieve short cycle times, overcome The effect of long cycle and simple method

Inactive Publication Date: 2007-09-12
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can obviously inhibit the mitosis of epidermal keratinocytes and make the epidermal layer thin (see the literature Wrench R. Epidermal thinning: evaluation of commercial corticosteroids. ArchDermatol Res, 1980, 267 (1): 7-24.), and has an effect on epidermal Langerhans It also has a certain inhibitory effect on Langerhans cells, promotes the migration of Langerhans cells and reduces the density of Langerhans cells in the epidermis. However, long-term administration of large doses will cause adverse reactions such as folliculitis and skin atrophy.

Method used

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  • Constructing method of model of lacking of Langerhans cells of small rat skin
  • Constructing method of model of lacking of Langerhans cells of small rat skin
  • Constructing method of model of lacking of Langerhans cells of small rat skin

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Experimental program
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Effect test

Embodiment 1

[0016] 1. Experimental animals and materials:

[0017] BALB / c mice, (6-8 weeks old, SPF grade, male), were purchased from Shanghai Xipuer-Bicai Laboratory Animal Co., Ltd., animal certificate number: SCXK (Shanghai) 2003-0002.

[0018] Drug: 0.2 mg / g (0.02%) clobetasol propionate ointment (hospital preparation of Changhai Hospital Affiliated to Second Military Medical University), document number (Nanzhizi (2006) F50030).

[0019] 2. Experimental method:

[0020] 40 BALB / c mice were randomly divided into 5 groups, including 8 normal control groups, and 4 groups of modeling components, 8 mice in each group, which were administered transdermally for 6 days, 8 days, and 10 days, respectively. and 12 days. All experimental mice were treated with hair removal on the chest and abdomen before the experiment, and then they were reared for 48-72 hours. The normal control group was fed routinely throughout the experiment, and the rest of the groups were smeared with clobetasol propio...

Embodiment 2

[0025] 1. Experimental animals and materials:

[0026] BALB / c mice, (6-8 weeks old, SPF grade, male), were purchased from Shanghai Xipuer-Bicai Laboratory Animal Co., Ltd., animal certificate number: SCXK (Shanghai) 2003-0002.

[0027] Drug: 0.2mg / g (0.02%) clobetasol propionate ointment (hospital preparation of Changhai Hospital Affiliated to Second Military Medical University), approval number is (Nanzhizi (2006) F50030).

[0028] (2wu / g) Recombinant Human Interferon α2b Cream (Recombinant Human Interferon α2bCream, Anhui Anke Bioengineering (Group) Co., Ltd.), the approval number is (Guoyao Zhunzi S20020032).

[0029] 2. Experimental method:

[0030]100 BALB / c mice were randomly divided into 20 groups according to body weight, 5 in each group, including 10 groups of normal mice and 10 groups of model mice, 50 in each group. All the experimental mice should be treated with hair removal on the chest and abdomen before the experiment, and then fed for 48-72 hours. 10 groups...

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Abstract

The present invention belongs to the field of medicine evaluating and detecting technology, and is especially process of constructing mouse skin Langerhans cell deletion model. Langerhans cell takes an important role in skin immune system. The mouse skin Langerhans cell deletion model constructing process includes adopting clobetasol propionate ointment as the model forming medicine and smearing it homogeneously onto the breast and abdominal skin of unhaired mouse. The histochemical staining and immunohistochemical experiments show that the said mouse model can well simulate the skin Langerhans cell deletion status, and may be used in screening skin immune medicine and in researching skin immune system inhibiting mechanism and medicine action mechanism. The present invention has simple process, short period, high repeatability, low cost and other advantages.

Description

Technical field: [0001] The invention belongs to the technical field of medical evaluation and detection, and in particular relates to an animal model construction method for screening and evaluating drugs for skin immune diseases and studying the mechanism of skin immune deficiency. Background technique: [0002] Research showing the importance of the skin in the overall immune system has provided a huge boost to both immunology and dermatology. The status of skin immunity research in the whole immune system also highlights its importance. [0003] In addition to the physical and biochemical barriers that have been discovered, skin tissue also has strong non-specific and specific immune functions. In recent years, studies have shown that the skin is a unique immune organ with unique immune functions. The Skin Immune System (SIS) proposed by BOS in 1986 established the important position of the skin in the entire immune system, which will have a profound impact on both immu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K31/57
Inventor 胡晋红李晋朱全刚
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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