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Skin type lupus erythematosus mouse model and construction method and application thereof

A construction method and technology for lupus erythematosus, which can be used in phototherapy, veterinary instruments, medical science, etc., can solve problems such as limitations, modeling success rate and efficiency limitations, and achieve the effect of accelerating clinical transformation.

Active Publication Date: 2021-09-21
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This makes modeling largely limited to the acquisition of qualified modeling raw materials, and has certain limitations in the success rate and efficiency of modeling

Method used

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  • Skin type lupus erythematosus mouse model and construction method and application thereof
  • Skin type lupus erythematosus mouse model and construction method and application thereof
  • Skin type lupus erythematosus mouse model and construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Embodiment 1: Construction method of experimental animal model of cutaneous lupus erythematosus

[0084] 1) Order SPF-grade female C57BL / 6 mice and Balb / c mice with animal quality certificates from formal production units. The age is 8W. Keep the outer packaging intact and spray disinfectant spray. After 30 minutes of ultraviolet sterilization, enter the SPF grade barrier environment, and then the experimental operations involved in the experiment of C57BL / 6 mice and Balb / c mice were carried out in this barrier environment;

[0085] All C57BL / 6 mice were weighed and recorded and arranged according to body weight from small to large, and the C57BL / 6 mice were randomly divided into Pristane i.c.+UVB group, PBS i.c.+UVB group, and Pristane i.c. group by random number table method; among them: Pristanei.c.+UVB group and Pristane i.c group were intradermally injected with Pristane 0.25ml at multiple points, PBS i.c.+UVB group was intradermally injected with PBS buffer 0.25ml...

Embodiment 2

[0090] Example 2: Verification experiment of the mouse model of cutaneous lupus erythematosus in Example 1

[0091] 2.1 Preparation of Paraffin Sections of Skin and Kidney Tissues of CLE Experimental Mice

[0092] 1) Euthanize the CLE experimental mice, cut a small piece of skin lesion tissue with a sharp blade, cut the whole kidney from the middle of the coronal plane, rinse with pre-cooled normal saline, and quickly put the skin tissue and kidney tissue in more than 4% Fix in POM at room temperature for 24 hours;

[0093] 2) Rinse the fixed skin lesions with running water;

[0094] 3) The washed specimens were soaked and dehydrated in sequence by 50% alcohol → 75% alcohol → 95% alcohol (I) → 95% alcohol (II) → absolute alcohol (I) → absolute alcohol (II). 2 hours;

[0095] 4) The dehydrated skin tissue was soaked in 1:1 absolute alcohol and xylene → xylene (I) → xylene (II) for 1 hour in sequence, and the tissue was visible in a transparent state;

[0096] 5) Melt three ...

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Abstract

The invention provides a skin type lupus erythematosus mouse model and a construction method and application thereof. According to the skin type lupus erythematosus mouse model constructed by the construction method, pristane is combined with ultraviolet (UVB) stimulation to construct the skin type lupus erythematosus mouse model. A C57BL / 6 mouse is continuously irradiated by a proper amount of UVB after being subjected to Pristane intradermal immune treatment. The skin type lupus erythematosus mouse model better simulates immune response generated by abnormal activation of mouse skin immune cells under UVB stimulation. The model can be used for discussing the occurrence mechanism of the disease progress of lupus erythematosus, researching and developing drugs for targeting lupus skin lesion and the like, and is of great significance in the aspect of accelerating and promoting scientific research and clinical transformation.

Description

technical field [0001] The invention belongs to the technical field of animal experiment models and their preparation, and in particular relates to a mouse model of skin type lupus erythematosus and its construction method and application. Background technique [0002] Pristane, also known as 2,6,10,14-tetramethylpentadecane, TMPD, is an isoprenoid alkane compound that can be found in plants, marine organisms, and by-products of petroleum distillation . At the same time, Pristane is also a membrane-activating compound that interacts with phospholipid bilayers. In both in vitro and in vivo conditions, Pristane can induce apoptosis in mouse lymphoid cell lines and peritoneal exudate cells, suggesting that Pristane-induced apoptosis provides sufficient self-antigen substrates, resulting in the interaction with interferon-α and Immune disorders associated with overproduction of β (IFN-α and IFN-β). Since Satoh M used Pristane to induce symptoms similar to human systemic lupus...

Claims

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Application Information

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IPC IPC(8): A01K67/02A61D7/00A61N5/06
CPCA01K67/02A61N5/06A61D7/00A61N2005/0661
Inventor 陆前进吴海竞周胜男李倩文赵明
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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