Application of 6-hydroxy dopamine (6-OHDA) to building of depigmentation model and method

A technology for depigmentation of hydroxydopamine and pigment, which can be used in pharmaceutical formulations, preparations for in vivo experiments, compound screening/testing, etc., and can solve problems such as deletion and apoptosis.

Active Publication Date: 2017-03-29
HANGZHOU THIRD HOSPITAL
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Problems solved by technology

Numerous studies have shown that 6-OHDA can induce

Method used

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  • Application of 6-hydroxy dopamine (6-OHDA) to building of depigmentation model and method
  • Application of 6-hydroxy dopamine (6-OHDA) to building of depigmentation model and method
  • Application of 6-hydroxy dopamine (6-OHDA) to building of depigmentation model and method

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Embodiment 1

[0027] 1. Experimental materials

[0028] 1.1 Experimental animals: clean grade C57BL / 6 mice, female, 3 weeks old, 15-20 g / mouse, purchased from the Animal Experiment Center of Zhejiang University of Traditional Chinese Medicine. The animals were randomly divided into a model group and a control group, with 3 animals in the model group and 3 animals in the normal control group. The experimental animals were reared in separate cages, with 3 animals per cage.

[0029] 1.2 Basic feed: mouse feed (provided by the Animal Experiment Center of Zhejiang University of Traditional Chinese Medicine)

[0030] 1.3 Reagents: 6-OHDA (purchased from Sigma, USA), normal saline and Vc solution were purchased from the pharmacy of Hangzhou Third People's Hospital. Dissolve 250mg of 6-OHDA in 4ml of physiological saline-L-ascorbic acid (Vc) solvent (0.9% w / v physiological saline-0.1% w / v Vc liquid), prepare a 400mM stock solution, and keep away from light at -20°C save;

[0031] The melanin st...

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Abstract

The invention relates to application of 6-hydroxy dopamine (6-OHDA) to building of a depigmentation model and a method for building the depigmentation model through the 6-OHDA. The 6-OHDA-induced mouse skin depigmentation model can be used for analyzing the pathogenesis of depigmentation diseases (including but not specific to vitiligo) and screening medicines for curing the diseases. Application of the 6-OHDA to building of the depigmentation model and the method for building the depigmentation model through the 6-OHDA have the beneficial effects that the 6-OHDA can be used for successfully building the depigmentation model and providing potential animal models for vitiligo basic and clinic research, and a foundation is provided for screening of antioxidized medicines and anti-vitiligo medicines.

Description

[0001] (1) Technical field [0002] The invention relates to the application of 6-hydroxydopamine (6-OHDA) in constructing a depigmentation model, and a method for constructing a depigmentation model by utilizing 6-OHDA. [0003] (2) Background technology [0004] Vitiligo is a pigmented disordered skin disease caused by the loss of epidermal melanocytes. Its pathogenesis is unclear and its clinical cure is difficult. In recent years, people have gained a new understanding of the mechanism of melanocyte loss: endogenous damage leads to immune response, which in turn causes melanocyte self-destruction, leading to vitiligo (J Invest Dermatol.2005,124(4):798-806. ). Oxidative stress can easily cause cell damage, and the phenomenon of oxidation-antioxidant imbalance has been found in both advanced and stable vitiligo patients, which indicates that oxidative stress has an important impact on the pathogenesis and even the progression of vitiligo, and these The study of the phenomen...

Claims

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Application Information

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IPC IPC(8): A61K49/00
CPCA61K49/0008
Inventor 关翠萍许爱娥许文
Owner HANGZHOU THIRD HOSPITAL
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