Construction method for mouse skin allergy animal model

Abstract

The invention belongs to medical evaluation and detection technology field. It concerns in particular to the construction method of animal model for skin allergy therapeutic drug screen and skin allergy mechanism study. The skin allergic mouse model is built by injecting anti DNP IgE monoclonal antibody to BALB / c mouse caudal vein and provoking the action by making 2,4 dinitroflurobenzene as antigen. The model can imitate the skin allergy dynamic reaction whole process favorably, therefore, it can be used for the screening of skin allergy treating drug and study of skin allergy pathogenesis and drug action mechanism.

Description

technical field [0001] The invention belongs to the technical field of medical evaluation and detection, and in particular relates to an animal model construction method for screening and treating skin allergy drugs and studying the mechanism of skin allergy. Background technique [0002] Skin allergy is an antigen-antibody reaction caused by allergens stimulating the body. When an external allergen enters the body, it activates the T lymphatic system and B lymphatic system, induces mast cell degranulation, releases allergic inflammatory mediators such as histamine and bradykinin, and produces allergic reactions, often manifested as symptoms such as skin allergies. In recent years, due to the aggravation of environmental pollution, the abuse of chemicals, and the increase of external harmful substances, the incidence of skin allergies has been increasing year by year. Although the disease has no direct threat to life safety, it seriously affects the quality of life of patie...

AI Technical Summary

Problems solved by technology

Although the disease has no direct threat to life safety, it seriously affects the quality of life of patients due to its complex etiology, protracted intractability and high incidence.

[0003] The pathogenesis of allergic diseases is still not completely clear, and the drug treatment of skin allergies is still limited to hormones and traditional antihistamines. reaction, mouse passive skin allergic reaction, mouse ear xenogeneic passive skin allergic reaction and other models, these models use ovalbumin or trichosanthin for injection as antigen, pertussis vaccine or aluminum hydroxide gel as allergen, firstly Animals are first sensitized to prepare antiserum, and then passive skin sensitization, excitation, and modeling are performed. The required period is long, the model preparation is complicated, and the molding rate is not high. There are also false negative reactions to individual drugs.

Evaluation indicators are highly subjective, and it is difficult to fully reflect the pathogenesis of skin allergies

This severely restricts researchers from large-scale screening of skin allergy drugs, and in-depth research on the pathogenesis of skin allergy and the mechanism of action of drugs.

Images