Construction method for mouse skin allergy animal model

A construction method and skin allergy technology, applied in the field of medical evaluation and detection, can solve the problems of long required period, high morbidity, and restrictive mechanism, and achieve the effects of good reproducibility, short period and simple method.

Inactive Publication Date: 2005-07-06
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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Problems solved by technology

Although the disease has no direct threat to life safety, it seriously affects the quality of life of patients due to its complex etiology, protracted intractability and high incidence.
[0003] The pathogenesis of allergic diseases is still not completely clear, and the drug treatment of skin allergies is still limited to hormones and traditional antihistamines. reaction, mouse passive skin allergic reaction, mouse ear xenogeneic passive skin allergic reaction and other models, these models use ovalbumin or trichosanthin for injection as antigen, pertussis vaccine or aluminum hydroxide gel as ...

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  • Construction method for mouse skin allergy animal model

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Embodiment 1

[0014] 1. Experimental animals and materials:

[0015] BALB / c mice, (6 weeks old, SPF grade, female), were purchased from Shanghai Experimental Animal Center, Chinese Academy of Sciences, animal certificate number: SCXK (Shanghai) 2003-0003.

[0016] Antibody: anti-DNP IgE monoclonal antibody (Sigma Company), diluted to 1:5000 with pH 7.4 PBS when used.

[0017] Antigen: 0.15% 2,4 dinitrofluorobenzene solution, the solvent is acetone: olive oil = 3:1.

[0018] Drug: cetirizine hydrochloride (Jiangsu Lianke Pharmaceutical Factory), the drug concentration of the high-dose group is 1 mg / ml, and the drug concentration of the small-dose group is 0.1 mg / ml.

[0019] 2. Experimental method: 60 BALB / c mice were used to measure the thickness of both ears with a thickness gauge 24 hours before the challenge, as the basic value. The mice were randomly divided into 4 groups, including 6 normal control groups, model group, cetirizine hydrochloride high-dose group (10 mg·kg -1 ), cetiriz...

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Abstract

The invention belongs to medical evaluation and detection technology field. It concerns in particular to the construction method of animal model for skin allergy therapeutic drug screen and skin allergy mechanism study. The skin allergic mouse model is built by injecting anti DNP IgE monoclonal antibody to BALB/c mouse caudal vein and provoking the action by making 2,4 dinitroflurobenzene as antigen. The model can imitate the skin allergy dynamic reaction whole process favorably, therefore, it can be used for the screening of skin allergy treating drug and study of skin allergy pathogenesis and drug action mechanism.

Description

technical field [0001] The invention belongs to the technical field of medical evaluation and detection, and in particular relates to an animal model construction method for screening and treating skin allergy drugs and studying the mechanism of skin allergy. Background technique [0002] Skin allergy is an antigen-antibody reaction caused by allergens stimulating the body. When an external allergen enters the body, it activates the T lymphatic system and B lymphatic system, induces mast cell degranulation, releases allergic inflammatory mediators such as histamine and bradykinin, and produces allergic reactions, often manifested as symptoms such as skin allergies. In recent years, due to the aggravation of environmental pollution, the abuse of chemicals, and the increase of external harmful substances, the incidence of skin allergies has been increasing year by year. Although the disease has no direct threat to life safety, it seriously affects the quality of life of patie...

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Application Information

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IPC IPC(8): A61K49/00
Inventor 胡晋红刘继勇朱全刚孙华君
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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