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Application of soracan gum in inhibiting skin injury of ultraviolet ray

A technology for skin damage and ultraviolet rays, applied in the fields of medicine and cosmetics, can solve the problems of many chemical sunscreen additives, easy to block pores, affect the appearance and other problems, achieve excellent anti-ultraviolet function, improve survival rate, and increase the effect of tolerance

Inactive Publication Date: 2014-04-23
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some very fine mineral particles are commonly used in physical sunscreens, such as zinc oxide, titanium dioxide, etc. At the same time, some talcum powder and calcium are added to form a protective film to scatter and absorb ultraviolet rays. The disadvantage is that it must be applied Very thick, greasy, easy to clog pores, and affect the appearance due to sweating and melting in the sun
Chemical sunscreens use chemical reactions to absorb ultraviolet rays in the sun for the skin. Chemical sunscreens have a natural effect after use and appear relatively light and clear. This is the biggest advantage of chemical sunscreens. The disadvantages are: there are many chemical sunscreen additives, some of which are effective The ingredients may cause allergies to individual skin, and it will take effect half an hour after application. Long-term use will cause certain damage to the skin

Method used

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  • Application of soracan gum in inhibiting skin injury of ultraviolet ray
  • Application of soracan gum in inhibiting skin injury of ultraviolet ray
  • Application of soracan gum in inhibiting skin injury of ultraviolet ray

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation of embodiment 1 Sola gum solution

[0022] The preparation of Sola gum solution comprises the following steps:

[0023] 1) The crude Sola gum was purified by the improved Sevag method. The purification steps are:

[0024] a. Prepare 0.5% Sola gum solution, heat (105°C, 15min), and when cooled to room temperature, 7000r / 10min, take the supernatant to remove residual bacteria.

[0025] b. Add pH4.0 acetic acid---sodium acetate solution at a ratio of 1:30 to remove DNA.

[0026] c. Mix the supernatant with phenol-chloroform solution (prepared as a mixed solution at a ratio of 1:1) according to the ratio of 1:1, shake vigorously for about 15 minutes, 7000r / 10min, and take the supernatant. Repeat this step 2-3 times to completely remove the protein.

[0027] d. Add 2 times the volume of industrial alcohol to the supernatant, the sugar flocculates into a ball, remove it, and squeeze out the alcohol.

[0028] e. Wash the precipitate with 75% alcohol for 2-...

Embodiment 2

[0031] Example 2 Sola gum to research on cell viability irradiated by ultraviolet UVA

[0032] The experimental cells were NIH3T3 mouse fibroblasts. Culture under standard experimental conditions: cells were cultured in DMEM high-glucose medium (complete medium) containing 10% calf serum and 1% penicillin and streptomycin at 37°C, 5% CO 2 In the biochemical incubator, the medium was changed every 2-3 days. Cells were digested and passaged with trypsin solution containing 0.25% trypsin-EDTA.

[0033] In the experiment, cells were treated differently, and ultraviolet UVA irradiation caused cell damage. By detecting the survival rate of cells after ultraviolet UVA irradiation, the degree of cell damage and the degree of protection of cells by Sola gum were evaluated.

[0034] NIH3T3 mouse fibroblasts in 2×10 4 Densities were seeded in 96-well plates, and the setup was divided into 6 groups of 5 wells. The experimental grouping situation is: CK, UVA, and Salecan components are...

Embodiment 3

[0035] Example 3 Sola gum to the research of mouse skin model after ultraviolet irradiation

[0036] The experimental animals were 8-week-old male C57BL / 6J mice. Raised under standard experimental conditions: 12-hour light-12-hour dark cycle, free access to water and food. All experimental mice were anesthetized by intraperitoneal injection of 10% chloral hydrate (4ml / kg). When the anesthesia was sufficient, the mice were fixed with their backs facing upward. With the back spine as the middle line, use scissors to cut off the mouse hair on the back, and the size is about 2×2cm. One week later, the mice were randomly divided into 3 groups, including CK group, UVA group and Salecan group. Among them, the CK group was the normal control group, and the UVA group was the ultraviolet UVA irradiation group. These two groups were given corresponding solvents during the experiment (ie distilled water); the Salecan group was the Sola gum solution treatment group, that is, before recei...

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Abstract

The invention discloses application of soracan gum in inhibiting skin injury of ultraviolet ray, and relates to application of soracan gum with different mass concentrations in protecting skin from injury due to ultraviolet ray UVA. A cell model adopted in the invention is an ultraviolet ray UVA induced cell injury model, wherein the dosage of soracan gum is 0.625-5 mu g / mu l; a mouse model adopted in the invention is an ultraviolet ray UVA induced mouse skin injury model, and the dosage of the soracan gum is 200mu l of 1 percent solution, the soracan gum can be used for improving the cell survival rate of cells in ultraviolet radiation, reducing inflammation factors in mouse skin and improving gene expression of synthetic collagen, so that skin injury caused by ultraviolet ray UVA can be inhibited. Cell and animal experiments prove that soracan gum has a remarkable effect of inhibiting skin injury of ultraviolet ray, so that soracan gum can be used in processes of preventing and repairing skin injury due to ultraviolet ray UVA, and can be used for preparing anti-ultraviolet radiation injury medicines and anti-ultraviolet radiation cosmetics.

Description

technical field [0001] The invention belongs to the technical fields of medicine and cosmetics, and relates to the application of Sola gum in inhibiting skin damage caused by ultraviolet rays. Background technique [0002] Ultraviolet rays are the general term for radiation with wavelengths from 10nm to 400nm in the electromagnetic spectrum. According to the different wavelengths of ultraviolet rays, ultraviolet rays are mainly divided into three regions, namely short-wave ultraviolet (UVC), medium-wave ultraviolet (UVB) and long-wave ultraviolet (UVA). The degree of penetration of ultraviolet rays into human skin is different. Long-wave ultraviolet rays can reach deep into the dermis, causing skin melanin, causing skin tanning, lipid and collagen damage, and long-term accumulation, which is the main cause of skin aging and serious damage. [0003] At present, the anti-ultraviolet sunscreen products on the market mainly include physical sunscreens, chemical sunscreens and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/716A61P17/16A61K8/73A61Q17/04
Inventor 张建法余晓红詹亦贝陈金苹周梦怡
Owner NANJING UNIV OF SCI & TECH
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