Non-diagnostic evaluation method after mouse back psoriasis model construction

Abstract

The invention provides a non-diagnostic evaluation method for a mouse back psoriasis model after construction, which comprises the following steps: S1, determining the percentage of a mouse skin lesion area in a drug application area skin lesion area, and scoring by 0-2 scores according to a skin lesion area proportion B, 0% = 0 score, 1%-30% = 1 score, and 31%-100% = 2 score; s2, determining an erythema score E, wherein the E is divided into 0-2 scores according to the color depth of the erythema; s3, a scale score D is determined, scoring is carried out according to the scale covering skin damage area, the scale covering with no scale is equal to 0 score, the scale covering with 30% of skin damage is equal to 1 score, the scale covering with 60% of skin damage is equal to 2 score, and the scale covering with 90% or above of skin damage is equal to 3 score; s4, determining a plaque score I, performing scoring by 0-3 scores according to the size of the plaque, and if no is equal to 0 score, determining the average diameter of the plaque as lt; 2 mm is equal to 1 minute, the average diameter of the plaque is 2-4 mm and is equal to 2 minutes, and the average diameter of the plaque is gt; 4 mm is equal to 3 minutes; s5, the skin lesion severity S is determined, S = B + E + D + I, the mouse back skin psoriasis inflammation severity is more accurately described and quantitatively compared according to the S score, and intuitive and accurate statistical analysis is facilitated.

Description

technical field [0001] The invention relates to the field of psoriasis research, in particular to a method for evaluating a non-diagnostic mouse back psoriasis model after construction. Background technique [0002] Psoriasis is an immune-mediated chronic inflammatory skin disease. In the study of psoriasis, the construction of animal models of psoriasis mainly relies on local drug induction and application at the gene level. We chose to apply imiquimod to the back skin of mice. After induction, erythema and scales were produced; histologically, it resulted in excessive proliferation of keratinocytes, neovascularization, and immune cell infiltration, with similar Phenotypic and pathological features of human psoriasis. Clinically, the patient's current condition is evaluated by scoring the patient's skin lesion area and skin lesion severity, that is, the PASI score. The method is to divide the human body into four parts: head and neck, trunk, upper extremities and lower e...

AI Technical Summary

Problems solved by technology

However, due to the actual needs and conditional constraints in the research process, we only selected the phenotypic changes of the back skin of the mice for scoring. In addition, considering the short pathogenic cycle of the induced mice, the peak of the phenotype can usually be reached in about five days , is quite different from the application of PASI scores in clinical patients. Therefore, in order to make our research data more effective and reflect the pathological phenotype changes of mice more accurately, we aimed at the psoriasis on the back of experimental mice. Disease model, invent a new scoring method

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