Preparation method of new compound in sabia parviflora and application

A kind of technology of fenugreek and compound, which is applied in the field of medicine, and can solve the problems of unknown active ingredients and unclear active substances of liver diseases

Active Publication Date: 2017-06-20
JIANGXI HERBFINE HI TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its related varieties are mostly used clinically for diseases related to the liver. However, the main active ingredients in Qingfengteng are unknown at present, and the active substances of its liver diseases are still unclear

Method used

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  • Preparation method of new compound in sabia parviflora and application
  • Preparation method of new compound in sabia parviflora and application
  • Preparation method of new compound in sabia parviflora and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preparation method of a kind of novel compound in Teng chrysanthemum, comprises the following steps:

[0017] (1) Get 100kg of dried fenugreek medicinal material, add 12 times the concentration of 70% ethanol to extract three times, the time is respectively 5 hours, 3 hours, 3 hours, filter, combine the extracts, concentrate under reduced pressure until no alcohol smell, get the extract;

[0018] (2) taking the extract of step (1) and adding concentration to 20-50% ethanol for solubilization, centrifuging with a centrifuge to remove insoluble matter to obtain a supernatant;

[0019] (3) Take the supernatant obtained in step (2) and put it on a macroporous resin column, first elute 5 column volumes with 30% ethanol-water to obtain fraction 1, then elute 5 columns with 50% ethanol-water volume, concentrated, and dried to obtain Fraction 2, which was subjected to 200-300-mesh silica gel column chromatography, followed by a mixture of dichloromethane and methanol in vo...

Embodiment 2

[0025] Embodiment 2 drug efficacy experimental research

[0026] 1. Experimental materials and animals

[0027] 1. Drugs and reagents

[0028] Alanine aminotransferase assay kit (R1, R2) (Japan Wako Pure Chemical Industries, Ltd.), aspartate aminotransferase assay kit (R1, R2) (Japan Wako Pure Chemical Industries, Ltd.), superoxide dismutase (SOD), Malondialdehyde (MDA) kit (purchased from Nanjing Jiancheng Institute of Bioengineering); carbon tetrachloride (analytical pure), prepared into 0.1% peanut oil solution with peanut oil before use; D-galactosamine (Shanghai Runcheng Biotechnology Co., Ltd.), before use, be made into 10% aqueous solution with normal saline; Bicyclol sheet (Beijing Xiehe Pharmaceutical Factory), before use, be ground into fine powder and make 1.25mg / ml suspension with 0.5% CMC-Na liquid.

[0029] 2. Experimental animals

[0030] Clean-grade ICR mice, weighing 18-22g, were purchased from Hunan Slack Jingda Experimental Animal Co., Ltd., certificate ...

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PUM

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Abstract

The invention relates to a preparation method and application of a new compound in sabia parviflora. Pharmacological research indicates that the referred new compound can lower the contents of ALT and AST in CC14 induced acute hepatic injury model mice, D-amino galactose induced acute hepatic injury model mice and ethanol induced hepatic injury model mice, lower hepatic tissue MDA content, and enhance liver SOD activity, and has an obvious protection effect on hepatic injury. The new compound is expected to be developed as a medicine or healthcare product with a hepatoprotective effect.

Description

technical field [0001] The invention relates to a preparation method and application of a new compound in Qingfengteng, belonging to the technical field of medicine. Background technique [0002] Sabia parviflora Wall.ex Roxb. The small flower Qingfengteng produced in Guizhou is a folk medicine of the Buyi and Miao nationalities. It is mainly distributed in Xingyi City, Anlong County, Ceheng County, Wangmo County and other places in Guizhou. Its rhizomes and leaves can be used as medicine. Anti-inflammatory and analgesic effects, the treatment of viral hepatitis A and B has remarkable curative effect, and the side effects are small. Its related varieties are mostly used clinically for diseases related to the liver. However, the main active ingredients in Qingfengteng are unknown at present, and the active substances of its liver diseases are still unclear. Contents of the invention [0003] The purpose of the present invention is to carry out in-depth research on the che...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/92A61K31/352A61P1/16
CPCC07D311/92
Inventor 李志峰冯育林王琦欧阳辉何明珍杨世林樊东辉俞双燕
Owner JIANGXI HERBFINE HI TECH
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