Abalone viscera acidic polysaccharose and health-care product containing abalone viscera acidic polysaccharose and application of abalone viscera acidic polysaccharose

A technology of acidic polysaccharides and abalone viscera, applied in the field of polysaccharides, can solve environmental pollution, reduce the value of abalone and other problems, and achieve the effect of promoting proliferation

Active Publication Date: 2013-01-30
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Abalone viscera accounts for 15-25% of the total weight of abalone. As a waste in the process of abalone processing,...

Method used

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  • Abalone viscera acidic polysaccharose and health-care product containing abalone viscera acidic polysaccharose and application of abalone viscera acidic polysaccharose
  • Abalone viscera acidic polysaccharose and health-care product containing abalone viscera acidic polysaccharose and application of abalone viscera acidic polysaccharose
  • Abalone viscera acidic polysaccharose and health-care product containing abalone viscera acidic polysaccharose and application of abalone viscera acidic polysaccharose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Extraction and separation and purification of acidic polysaccharides from abalone viscera, the following steps are carried out in sequence:

[0046] 1) Grind 100g of dried abalone viscera into powder, add 2000mL of mixed chloroform-methanol (V:V, 2:1) mixed solution, soak for 12h, filter, and dry the filter residue.

[0047] 2) Take the dried abalone viscera powder obtained in step 1), add 3000mL of mixed buffer solution, and add 10g of papain, and stir and react in a water bath at 60°C for 24 hours. The preparation method of the mixed buffer solution is as follows: - Add 5 mmol of EDTA and 5 mmol of cysteine ​​to sodium acetate buffer solution (pH 6.0).

[0048]3), centrifuge the enzymolysis product obtained in step 2) (2000g, 15min, 20°C), add 80mL of cetylpyridinium chloride (CPC) aqueous solution with a mass concentration of 10% to the obtained supernatant, and After being left for 24 hours, it was centrifuged (2000g, 15min), and the supernatant was disc...

Embodiment 2

[0053] Example 2 Structural Analysis of the Abalone Visceral Acidic Polysaccharides Obtained in Example 1

[0054] 1) Take 2.0 mg of acidic polysaccharide from abalone viscera obtained in Example 1 above into an ampoule bottle, add 1 mL of 2mol·L-1TFA, seal the tube with nitrogen gas, and hydrolyze at 110°C for 8 hours. Then cool to room temperature, evaporate TFA at 50°C, adjust to neutral with 2mol L-1 and 0.3mol L-1 NaOH solution successively, dilute to 1mL with ultrapure water, take 450μL and 50μL internal standard Lactose was derivatized with PMP. Chromatographic conditions: Chromatographic instrument: Agilent 1100 high performance liquid chromatograph, chromatographic column: ZORBAX EclipseXDB-C18 separation column (4.6×150mm, 5μm), detector: UV detector, 245nm, flow rate: 1.0mL·min-1, column Temperature: 25°C, mobile phase: solvent A: 10% (V / V) acetonitrile + 0.1mol L-1 ammonium acetate-acetic acid buffer (pH 5.5), solvent B: 25% (V / V) acetonitrile + 0.1 mol L-1 ammon...

Embodiment 3

[0069] Example 3 Acidic polysaccharides from abalone viscera promote cell proliferation activity

[0070] 1) MTT method to detect the effect of polysaccharides on the proliferation of HepG2 cells. HepG2 cells in the logarithmic growth phase were prepared with RPMI-1640 complete medium (containing 10% newborn bovine serum) at a density of 2×10 4 pcs mL -1 The cell suspension was inoculated in a 96-well culture plate, 100 μL / well. After 24 hours of adherence, the concentration was changed to 25 μg·mL -1 , 50μg·mL -1 , 100μg·mL -1 , 200μg·mL -1 and 400μg·mL -1 For each concentration of polysaccharides, 4 replicate wells were set up, 200 μL / well. Add the same amount of complete medium to the blank control group. 37°C, 5% CO 2 After culturing for 24h, 48h and 72h under the same conditions, discard the medium and add MTT solution (prepared in PBS solution, the final concentration is 0.5mg·mL -1 ). After continuing to cultivate for 4 hours, suck off the supernatant, add aci...

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Abstract

The invention provides abalone viscera acidic polysaccharose. According to the abalone viscera acidic polysaccharose, a main chain comprises rhamnose and glucuronic acid, wherein a C-2 position or C-3 position of the rhamnose in the main chain is subjected to sulphating; and the rhamnose in the main chain is connected with the rhamnose or galactose to form a branched chain structure. The abalone viscera acidic polysaccharose which is extracted from abalone viscera can promote the proliferation of hepatoma carcinoma cells (HepG2) in vitro obviously, and has a certain effect of dose dependency; and experiments of studying a repair effect of the abalone viscera acidic polysaccharose on the HepG2 cells which are subjected to oxidative damage and on rat hepatic tissues which are subjected to acute hepatic damage caused by carbon tetrachloride in vivo indicate that the abalone viscera acidic polysaccharose can be used as a functional factor for protecting liver.

Description

technical field [0001] The invention relates to a polysaccharide, in particular to a novel acidic polysaccharide extracted from abalone viscera. Background technique [0002] Abalone belongs to the phylum Mollusca, Gastropoda, Prosobranchia, Archaeogastropoda, Haliotidae, and Haliotis. Sea areas around the world have been named There are 216 species of abalone in China, about 30 common species, and 7 species are mainly distributed along the coast of China. Wrinkled plate abalone and variegated abalone are the representative species in the north and south of my country respectively. Compared with variegated abalone, the wrinkled plate abalone has higher economic value, and the most famous abalone produced in the coast of Shandong, known as "Denglai bream" in history . Abalone viscera accounts for 15-25% of the total weight of abalone. As a waste in the process of abalone processing, it is mostly discarded or made into feed, which not only pollutes the environment, but also r...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/737A61P1/16A61P35/00A23L1/09
Inventor 王玉明李国云武风娟薛长湖薛勇李兆杰徐杰王静凤常耀光唐庆娟
Owner OCEAN UNIV OF CHINA
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