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Decellularized epineurium catheter and preparation thereof as well as application to repairing of peripheral nerve injury

A technology for peripheral nerve injury and decellularization, which is applied in the field of biomaterials and nerve injury repair, can solve the problems of epineurial catheter function without literature report and the preparation of epineurial catheter without decellularization reported in literature, etc., so as to reduce peripheral nerve Adhesion, reduced incidence of neuroma, low immunogenic effect

Inactive Publication Date: 2017-01-25
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] There are no reports on the preparation of decellularized epineurium conduits; there are no reports on the role of decellularized epineurium conduits in the repair of peripheral nerve injuries

Method used

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  • Decellularized epineurium catheter and preparation thereof as well as application to repairing of peripheral nerve injury
  • Decellularized epineurium catheter and preparation thereof as well as application to repairing of peripheral nerve injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Preparation of decellularized epineurium catheters

[0034] Place several pieces of epineurium catheters in distilled water for shaking and rinsing for 6 hours.

[0035] Put the nerve into 10ml / L Triton X-100 (polyethylene glycol octylphenyl ether, purchased from Sigma company) aqueous solution and let it stand for 12h. After rinsing in distilled water for 3 times, put it into 40g / L deoxygenated Sodium cholate aqueous solution (purchased from Sigma) was shaken at room temperature for 24 h, and finally rinsed in distilled water for 3 times. 60 Co irradiation (20kGy) was sterilized for 12h, and stored at 4°C for later use.

Embodiment 2

[0036] Example 2. Study on Biological Properties of Decellularized Epineurium Conduits

[0037] (1) Observation of ultrastructure

[0038] The ultrastructure of the cross-section, longitudinal section and outer surface of the decellularized epineurium catheter was observed under scanning electron microscope and transmission electron microscope. The results showed that the collagen components in the decellularized epineurium ducts were well preserved and the cells were completely removed.

[0039] (2) Determination of porosity

[0040] The decellularized epineurium catheter was placed in a freeze dryer for 24 hours, and the porosity of the material was measured by the liquid substitution method. The details are as follows: Take 10 pieces of material, randomly select 5 points for cross-sectional diameter measurement and calculate the average value. The electronic balance weighs its mass (accurate to 4 decimal places), and weighs 5 times to find the average value. Immerse a s...

Embodiment 3

[0047] Example 3. Study on Immunogenicity of Decellularized Epineurium Catheters

[0048] The porcine decellularized epineurium catheter and the fresh epineurium catheter were respectively implanted into the paravertebral muscles of SD rats, and the samples were collected for histological observation at 1 to 4 weeks after the operation, and the degree of inflammatory response was evaluated by H.E. staining; immunohistochemistry was applied Methods The degree of infiltration of CD3, CD4 and CD8 positive T cells in the graft and surrounding tissues was observed, and the intensity of immune rejection was evaluated. The results showed that the inflammatory response and immune rejection in the decellularized epineurium catheter group were weaker than those in the fresh epineurium catheter group.

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Abstract

The invention belongs to the technical field of biological materials and nerve injury repairing, and particularly relates to a decellularized epineurium catheter and a preparation method thereof as well as application to repairing of a peripheral nerve injury. The biological characteristic of the decellularized epineurium catheter for a pig is researched from such aspects as the porosity, the swelling rate, the in vitro degradation rate and the cytotoxicity test, and the immunogenicity of the catheter is also researched. According to the decellularized epineurium catheter and the preparation method thereof, a rat sciatic nerve transversal injury model is built; after being subjected to microsurgical suturing, a nerve stump line is covered with the decellularized epineurium catheter so as to research the effects of promoting regeneration of nerves, preventing nerve adhesion and formation of nerve tumors. The invention provides the decellularized epineurium catheter which can effectively reduce surrounding nerve adhesion and reduce the occurrence rate of the nerve tumors, and a novel repairing material is provided for repairing of war traumatic surrounding nerve injuries and treatment of causalgia caused by the nerve injuries.

Description

technical field [0001] The invention belongs to the technical field of biological materials and nerve damage repair, and in particular relates to a decellularized epineurium catheter, a preparation method thereof, and an application in repairing peripheral nerve damage. Background technique [0002] Peripheral nerve injuries are common in both peacetime and wartime. According to the statistics of war injuries in World War II, nerve injuries of extremities account for about 10% of the total number of traumas. In modern wars, modern traffic accidents, high-energy explosions and industrial accidents, the rate of nerve damage is higher. Peripheral nerve battle injuries have risen to 20% to 40% now, and about 60% of firearm injuries and fractures are combined with nerve injuries. Firearm-induced peripheral nerve injury can often cause nerve injury, which is similar to the clinical manifestations of nerve injury caused by other factors. After the injury, the motor and sensory fu...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
Inventor 刘芳张传森许家军杨向群张少成张志英蔺海燕
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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