Tilapia mossambica precision-cut liver slice injury model and construction method thereof

A technology of injury model and construction method, applied in artificial cell constructs, vertebrate cells, animal cells, etc., can solve the problem of tilapia precision liver slice injury model, and achieve the effect of reliable liver metabolism and induced toxicity in vitro

Pending Publication Date: 2021-04-20
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

The liver injury model induced by CTX has been widely used and studied clinica...

Method used

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  • Tilapia mossambica precision-cut liver slice injury model and construction method thereof
  • Tilapia mossambica precision-cut liver slice injury model and construction method thereof
  • Tilapia mossambica precision-cut liver slice injury model and construction method thereof

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Experimental program
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Embodiment 1

[0019] The construction method of tilapia precision liver slice injury model is as follows:

[0020] 1. Preparation of Tilapia Precision Liver Slices (PCLS):

[0021] Healthy tilapia with a body weight of 150±5 g were provided by the fish farm of the Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences. Raised in a circulating water system (each tank is 240L), the feeding conditions are (27±2)℃, pH6.8~7.6, DO(dissolvedoxygen)>5 mg / L, NH 3 2 S<0.01mg / L. The daily feeding amount is 2% of the fish body mass. Randomly selected tilapias were killed by anesthesia and bloodletting, sterilized with 75% alcohol, and their livers were taken from the ultra-clean workbench and placed in ice Krebs-Henseleit (pH 7.4) buffer solution saturated with oxygen in advance, after removing the envelope and coagulation , processed with a scalpel into a tissue block with a volume of 5 mm × 5 mm × 5 mm, and then embedded in 2.5% low-melting point glue. After the tissue em...

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Abstract

The invention discloses a tilapia mossambica precision-cut liver slice injury model and a construction method thereof. The method adopts cyclophosphamide to induce tilapia mossambica precision-cut liver slices, and the concentration of the cyclophosphamide is 25mM or below. Compared with the prior art, the method disclosed by the invention has the following advantages: (1) the cyclophosphamide-induced tilapia mossambica precision-cut liver slices are adopted for the first time to construct an injury model, and a basis is provided for researching a fish liver injury mechanism and screening and developing liver protection drugs; and (2) a tilapia mossambica precision-cut liver slice is adopted as an induction object of the method, the in-vivo state is simulated to the maximum extent, and the method is a more reliable model system for researching liver metabolism and in-vitro induction toxicity.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and relates to the preparation of liver slices, in particular to a tilapia precision liver slice injury model and a construction method thereof. Background technique [0002] With the continuous improvement of intensive farming and the increase of breeding density, the breeding environment is deteriorating day by day. At the same time, the abuse of antibiotics and pesticides has also caused frequent fish diseases, especially liver diseases. The liver is an important organ of fish material and energy metabolism, and also an important barrier organ of the body. Its detoxification and phagocytosis functions have important protective effects on the body. Liver damage or lesions often lead to metabolic dysfunction and reduced disease resistance of fish, which can easily cause outbreaks of secondary infectious diseases. Therefore, it is of great significance to study the pathogenesis of tilapia li...

Claims

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Application Information

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IPC IPC(8): C12N5/071
Inventor 曹丽萍贾睿杜金梁丁炜东
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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