Rat lung injury model for different severity degrees and for achieving induction of different doses of bleomycin through tracheal inside spraying

Abstract

The invention provides a rat lung injury model for different severity degrees and for achieving induction of different doses of bleomycin through tracheal inside spraying. The model comprises the following steps that rats are anesthetized, and after the rats reach the anesthetized state, the rats are immediately kept on an inclined face keeping table in a supine manner so that tracheal openings can be found smoothly through oral cavities of the rats; a cold light source is used for irradiating the lower jaws of the necks of the rats so as to obtain sufficient light during subsequent intraoraloperation; the tongues of the rats are pulled out, the tongue roots are pressed by tongue depressors, and the tracheal openings are found; and a tracheal inside atomizer is used, a needle-shaped headof the atomizer is directly inserted into the tracheas of the rats by 3-4 cm, the breathing rule of the rats is observed, bleomycin (10 mg/kg-80 mg/kg weight) of different doses is rapidly sprayed into the tracheas at the moment that the rats inhale, and then the rats are kept on the inclined face keeping table for 5-30 s. By means of the obtained rat lung injury model which is stable, controllable in death rate, better in consistency and capable of simulating different clinical severity degrees, a more reliable animal experiment material is provided for medical preclinical research.

Description

technical field [0001] The invention relates to an animal disease model, in particular to a rat lung injury model of different severity induced by intratracheal spraying of different doses of bleomycin. Background technique [0002] A stable lung injury model with controllable severity and mortality and good consistency is an important carrier and basis for preclinical research on drugs for lung diseases. At present, the commonly used rat lung injury model is to expose the trachea after neck surgery, puncture the trachea, and instill bleomycin through the trachea at a dose of 5 mg / kg. This model has a high mortality rate and a low molding rate, and the model is consistent. Sex is poor. Moreover, it cannot stably and well simulate the clinical state of lung injury with different severity, which also hinders the research on lung injury under different course of disease. Contents of the invention [0003] Technical problem to be solved: the purpose of this invention is to p...

AI Technical Summary

Problems solved by technology

At present, the commonly used rat lung injury model is to expose the trachea after neck surgery, puncture the trachea, and instill bleomycin through the trachea at a dose of 5 mg / kg. This model has a high mortality rate and a low molding rate, and the model is consistent. Poor sex

Moreover, it cannot stably and well simulate the clinical state of lung injury of different severity, which also hinders the research on lung injury under different disease courses.

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