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Tilapia oxidative stress liver injury model and construction method and application thereof

A technology for oxidative stress and construction methods, which can be applied in pharmaceutical formulations, compound screening/testing, preparations for in vivo experiments, etc., can solve the problem of constructing fish oxidative stress liver injury models that have not yet been discovered, and restrict fish liver injury Screening and development of anti-mechanism hepatoprotective drugs to achieve the effect of strong reproducibility and controllable culture environment

Pending Publication Date: 2019-08-02
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in fish, there are still relatively few studies on liver injury models, which severely restricts the mechanism of liver injury in fish and the screening and development of liver-protecting drugs.
At present, there is no method for constructing a fish model of oxidative stress liver injury
However, no H 2 o 2 Related reports on induced liver injury in fish

Method used

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  • Tilapia oxidative stress liver injury model and construction method and application thereof
  • Tilapia oxidative stress liver injury model and construction method and application thereof
  • Tilapia oxidative stress liver injury model and construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Experimental design

[0025] Healthy tilapia were cultured in a circulating water system and domesticated for 2 weeks. During the domestication period, the basic feed was fed, and the breeding conditions were: temperature 30±2°C, dissolved oxygen>6mg / L, pH 7.4–8.1, ammonia nitrogen content2 o 2 Prepare 4 different concentrations with saline: 0, 40, 120 and 300mM.

[0026] After domestication, the tilapia were randomly divided into 4 groups, and 4 different concentrations of H were injected intraperitoneally. 2 o 2 (0, 40, 120 and 300mM), the 0mM group was the control group (CK), 30 fish in each group, and the injection dose was 3mL / kg. 24 hours after injection, 15 fish were randomly selected in each group, and blood and liver tissues were collected after anesthesia. Determination of liver injury indicators in blood: aspartate transaminase (GOT) and alanine aminotransferase (GPT); determination of oxidative stress indicators in liver tissue: superoxide dismutase (...

Embodiment 2

[0037] 1. Experimental design

[0038] Based on this animal model, three hepatoprotective drugs were screened: baicalin, white peony extract and Bupleurum extract.

[0039] Healthy tilapia were cultured in a circulating water system and domesticated for 2 weeks. During the domestication period, the basic feed was fed, and the breeding conditions were: temperature 30±2°C, dissolved oxygen>6mg / L, pH 7.4–8.1, ammonia nitrogen content<0.2mg / L, nitrite content<0.2mg / L.

[0040] After domestication, the tilapia were randomly divided into 6 groups: control group, model group, baicalin group, white peony extract group and Bupleurum extract group. The control group and the model group were fed with basic feed, the baicalin group was fed with feed containing 1.4g / kg baicalin, the white peony extract group was fed with feed containing 1g / kg white peony extract, and the Bupleurum extract group was fed with Feed containing 3g / kg Bupleurum extract. After feeding for 60 days, fasting for ...

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Abstract

The invention discloses a tilapia oxidative stress liver injury model and a construction method and application thereof. The method is to construct the damage model by injecting H2O2. The advantages are that: (1) a tilapia source used in the method disclosed by the invention is the same, the culture environment is controllable, and thus the repeatability is high; (2) the method disclosed by the invention applies the H2O2 to construct the fish liver injury model for the first time, and the ideal tilapia experimental model capable of being used for studying the pathogenesis of liver disease andscreening related therapeutic drugs is obtained.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and relates to a method for constructing a fish injury model, in particular to a tilapia oxidative stress liver injury model and its construction method and application. Background technique [0002] The liver is the main organ of fish material and energy metabolism, and also an important barrier organ of the body. Its detoxification and phagocytosis functions have protective effects on the body. Liver damage or lesions often lead to metabolic dysfunction and reduced disease resistance, which can easily cause outbreaks of secondary infectious diseases. In recent years, liver diseases such as hepatobiliary syndrome, fatty liver disease and chemical liver injury have frequently occurred in many fishes, with a mortality rate of 50%-60%. The etiology is complex, but the common point is that they are all accompanied by severe oxidative stress ( oxidative stress). A variety of stress factors in t...

Claims

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Application Information

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IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/027A01K2207/20A01K2227/40A01K2267/03A61K49/0008
Inventor 贾睿殷国俊曹丽萍杜金梁
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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