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Diagnostic reagent for cervical cancer cell detection and preparation method thereof

A diagnostic reagent and technology for cervical cancer, which is applied in biological tests, material inspection products, and material analysis by observing the impact on chemical indicators, etc. problem, to achieve the effect of good stability, good clinical application prospects, and strong reducibility

Active Publication Date: 2015-04-01
SHENZHEN HUAZHONG BIOLOGICAL PHARMA MACHINERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this diagnostic reagent is not specific for the detection of epithelial tissue lesion cells in special parts, and there is also the problem of poor product stability

Method used

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  • Diagnostic reagent for cervical cancer cell detection and preparation method thereof
  • Diagnostic reagent for cervical cancer cell detection and preparation method thereof
  • Diagnostic reagent for cervical cancer cell detection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 is used for the preparation of the diagnostic reagent of cervical carcinogenesis cell detection

[0032] The diagnostic reagent used for the detection of cervical cancer cell of the present invention is composed of the following components by mass: 10 parts of glucose, 0.5 part of folic acid, 0.4 part of methylene blue, 0.8 part of lycopene, 0.5 part of 3mol / L sodium hydroxide aqueous solution, dimethyl 90 parts of sulfoxide, 4 parts of acetic acid, 0.01 part of dithiothreitol, and 0.05 part of sodium methyl p-hydroxybenzoate. Its preparation method comprises the following steps:

[0033] (1) Add 10 parts of glucose and 0.8 parts of lycopene into 70 parts of dimethyl sulfoxide, stir and dissolve;

[0034] (2) Prepare 0.5 part of 3mol / L sodium hydroxide aqueous solution, then add 0.5 part of folic acid into the sodium hydroxide aqueous solution, stir and dissolve;

[0035] (3) Add 4 parts of acetic acid into 20 parts of dimethyl sulfoxide, stir and dissolv...

Embodiment 2

[0039] Embodiment 2 is used for the mensuration of the diagnostic reagent stability of cervical canceration cell detection

[0040] Iodine direct titration method: Prepare and accurately draw 3mL of the sample solution to be tested, fully mix it with 3mL indicator starch aqueous solution (10g / L), and immediately titrate with I2 standard solution (0.025mol / L, diluted by 0.1mol / L I2 standard solution) To light yellow, no fading within 30s is the end point, record the volume of I2 standard solution consumed, and calculate the antioxidant capacity of the sample by comparing the volume of I2 standard solution consumed.

[0041]The diagnostic reagent for the detection of cervical cancer cells prepared in Example 1, the reagent sample in the patent "diagnostic reagent for detection of epithelial lesion cells and its preparation process and cotton swab (103808551A)" (100mL of the solution reagent contains methylene blue 0.37 %, fructose 4.69%, glucose 9.38%, vitamin C 1.00%, folic aci...

Embodiment 3

[0042] Embodiment 3 is used for the detection of cell carcinogenesis by the diagnostic reagent used for the detection of cervical cancer cells

[0043] The Hela cell line is derived from the adult cervix. It is a cancer cell line that can proliferate indefinitely. It is a model research cell line for tumor cells in biological and medical research. The HaCaT cell line is an immortalized cell line derived from adult human epidermis that exhibits a normal state of differentiation and provides an ideal model for studying the regulation of human epidermal cells. Although the HaCaT cell line exhibits obvious immortalization, it still does not have the characteristics of tumor cells and can be used as a model of normal cells.

[0044] Tumor cells have a high affinity with methylene blue, and the redox property of methylene blue makes methylene blue have different color-changing reaction spectra in the redox system of tumor cells.

[0045] (1) Good specificity

[0046] Scrape HaCaT ...

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Abstract

The invention discloses a diagnostic reagent for cervical cancer cell detection and a preparation method thereof. The diagnostic reagent comprises components in parts by mass as follows: 10 parts of glucose, 0.5 parts of folic acid, 0.4 parts of methylene blue, 0.8 parts of lycopene, 0.5 parts of a 3mol / L sodium hydroxide aqueous solution, 90 parts of dimethyl sulfoxide, 4 parts of acetic acid, 0.01 parts of dithiothreitol and 0.05 parts of sodium methyl parahydroxybenzoate. The preparation method comprises steps as follows: the glucose, the lycopene and acetic acid are dissolved in dimethyl sulfoxiderespectively; the folic acid is added to the sodium hydroxide aqueous solution for dissolution and then added to the dimethyl sulfoxide with theglucose and the lycopene dissolved, then methylene blue is added, stirred and dissolved, the dimethyl sulfoxide with theacetic acid dissolved is added, and the dithiothreitol and the sodium methyl parahydroxybenzoate are added after even mixing. The diagnostic reagent is convenient to use and high in sensitivity and stability, cotton swabs are dipped in the diagnostic reagent during usage, and the diagnostic detection rate is up to 90%.

Description

technical field [0001] The invention relates to a diagnostic reagent for cancerous cells, in particular to a diagnostic reagent for detecting cervical cancerous cells and a preparation method thereof. Background technique [0002] Cervical cancer is one of the common malignant tumors in women, ranking second among women's cancers in the world and the first among malignant tumors of the female reproductive system in my country. There are about 135,000 new cases of cervical cancer in China every year, accounting for 1 / 5 of the new cases of cervical cancer in the world. However, cervical cancer is currently the only cancer with a clear etiology among all human cancers that can be prevented and controlled. Through standardized anti-cancer screening, timely detection and treatment of precancerous lesions can prevent its development into cervical cancer. Therefore, how to improve the sensitivity and specificity of cervical cancer screening and meet the simple and economical requi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N21/78
CPCG01N21/78G01N33/52
Inventor 李立家张蔚高飞吕琼莹王雅佩李英南周泓文欢
Owner SHENZHEN HUAZHONG BIOLOGICAL PHARMA MACHINERY
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