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Application of lecithin superoxide dismutase composite in preparation of medicines for treating and/or preventing cardiac ischemic injury

A technology of superoxide and cardiac ischemia, applied in drug combinations, cardiovascular system diseases, pharmaceutical formulations, etc., can solve problems such as inability to scavenge oxygen free radicals, difficulty in obtaining ideal results, poor cell affinity, etc., to achieve The effect of reducing reperfusion injury, reducing infarct size, and reducing heart failure

Inactive Publication Date: 2011-02-09
BEIJING TIDE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the injury of myocardial cells caused by ischemia-reperfusion in the treatment of myocardial protection, on the one hand, there is no general prevention and treatment method for myocardial protection, and Na + / H + exchange inhibitor, Na + / Ca 2+ Exchange inhibitors, repolarization fluids, statins, and platelet inhibitors are used for treatment, but their mechanisms of action are all downstream of oxygen free radical damage, so it is difficult to obtain ideal results
On the other hand, although early researchers proposed the use of superoxide dismutase to treat ischemia-reperfusion injury, due to the extremely short half-life of superoxide dismutase in the blood and poor cell affinity when administered intravenously, superoxide dismutase cannot be cleared in vivo. The role of oxygen free radicals
However, there is no report on the application of PC-SOD compositions containing different numbers of lecithin (PC) molecules in the treatment of cardiac ischemic injury

Method used

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  • Application of lecithin superoxide dismutase composite in preparation of medicines for treating and/or preventing cardiac ischemic injury
  • Application of lecithin superoxide dismutase composite in preparation of medicines for treating and/or preventing cardiac ischemic injury
  • Application of lecithin superoxide dismutase composite in preparation of medicines for treating and/or preventing cardiac ischemic injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 The lecithinated superoxide dismutase composition was administered in different doses, and its pharmacodynamic study was carried out in the treatment of myocardial ischemia-reperfusion in Chinese minipigs

[0029] 1. Experimental materials

[0030] Experimental animals: 35 Chinese miniature pigs, purchased from Beijing Kexing Experimental Animal Breeding Center, male or female, weighing 20-30kg;

[0031] Experimental drug:

[0032] Test product: lecithinated superoxide dismutase composition freeze-dried preparation, its preparation method is as follows:

[0033] 4,500 mL of distilled water for injection was added to a 10 L flask, and 13 g of boric acid, 1.8 g of NaOH, and 68 g of KCl were sequentially added to dissolve. Then, an aqueous SOD solution (50 g, SOD concentration 56.8 mg / mL) was added, and while stirring at 210 rpm, 1000 mL of isopropanol at a temperature of 8° C. was added dropwise at a rate of 16.5 mL / min in the chromatography chamber to prepa...

Embodiment 2

[0075] Example 2 The lecithinated superoxide dismutase composition was administered 30 minutes and 3 minutes before reperfusion, respectively, and its pharmacodynamic study on the treatment of myocardial ischemia and reperfusion in Chinese miniature pigs was carried out

[0076]1. Experimental materials

[0077] Experimental animals: 28 small Chinese animals, purchased from Beijing Kexing Experimental Animal Breeding Center, male or female, weighing 20-30kg;

[0078] Experimental drug:

[0079] Test product: lecithinated superoxide dismutase composition freeze-dried preparation, its preparation method is as follows:

[0080] Add 6000 mL of distilled water for injection into a 20 L flask, and then add 15 g of boric acid, 2.3 g of NaOH and 76 g of KCl in order to dissolve. Then, an aqueous SOD solution (65 g, SOD concentration 60.3 mg / mL) was added, and while stirring at 210 rpm, 1000 mL of isopropanol at a rate of 16.5 mL / min was added dropwise at a temperature of 8° C. in ...

Embodiment 3

[0124] Example 3 Contrasting Pharmacodynamic Activity of PC-SOD Compositions Containing Different Numbers of PC Molecules and PC-SOD Compositions Containing the Same Number of PC Molecules

[0125] In order to verify that the pharmacodynamic activity of PC-SOD compositions containing different numbers of PC molecules is better than that of PC-SOD compositions containing the same number of PC molecules, the present invention measures the pharmacokinetics of blood drug concentration by ELISA method. Kinetic test for comparison, the drug-time curve is as follows Figure 6 shown.

[0126] 1. Experimental materials

[0127] Experimental animals: The experimental animals were 60 SPF grade Sprague-Dawley (SD) rats, purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., half male and half female, weighing 175-205g;

[0128] Experimental drug:

[0129] testing sample:

[0130] Lecithinated superoxide dismutase composition freeze-dried preparation, its pre...

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Abstract

The invention relates to application of a lecithin superoxide dismutase composite in preparation of medicines for treating and / or preventing cardiac ischemic injury, wherein the lecithin superoxide dismutase composite is dipolymer combined with different molecule numbers of lecithin, and the concrete method comprises the following steps of: establishing a cardiac ischemic injury animal model; preparing a cardiac injury induced model for verifying a function of the cardiac ischemic injury animal model in cardiac protection treatment under the asepsis condition, spreading sacculus, blocking the sacculus for a long time and loosening the sacculus through injecting a contrast agent into the small pig coronary artery left anterior descending branch balloon implant so as to respectively establish a myocardial infarction model and a myocardial injury model through blood liquid infusion; and injecting the lecithin superoxide dismutase composite containing different molecule numbers of lecithin into the animal model so as to observe a new function of treating cardiac injury.

Description

technical field [0001] The present invention relates to the new application of lecithinated superoxide dismutase (PC-SOD) composition, specifically, the present invention relates to the preparation of lecithinated superoxide dismutase composition in the preparation of treatment and / or prevention of cardiac ischemic injury application in medicines. Background technique [0002] The Chinese patent ZL200580034389.0 of the applicant's invention titled "lecithinated superoxide dismutase composition and its preparation method" discloses that the PC-SOD monomer in the lecithinated superoxide dismutase composition contains Copper and / or zinc coordination, the sulfhydryl group of cysteine ​​at position 111 is sulfurized by hydroxyethyl, and more than one amino group from human superoxide dismutase is represented by the following general formula (I): [0003] [0004] The lecithinated superoxide dismutase substituted by the lecithin residue indicated, the lecithinated superoxide d...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K47/24A61P39/06A61P9/10A61K38/44A61K47/54
Inventor 刘红星张扬肖萱杨青松郁亮齐连全周丽莹
Owner BEIJING TIDE PHARMA
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