Hernia patch, preparation method and application thereof in herniorrhaphy

A water-for-injection, allogeneic technology, applied in the field of tissue engineering of biomedical materials, can solve the problems of residual antigenic components, inconvenient storage and transportation, low elasticity, etc. Effects of elasticity and toughness

Active Publication Date: 2018-07-10
BEIJING JAYYALIFE BIOTECH CO LTD
View PDF3 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former removes cells and antigenic components in skin tissue by chemical detergents combined with other auxiliary reagents, such as removing cells in a solution containing EDTA and trypsin or DispaseII enzyme, and then incubating them in TritonX-100; the latter is prepared by Hypertonic sodium chloride and SDS preparation, but more antigenic components will remain
In addition, there are also preparation methods such as trypsin digestion method, repeated freezing and thawing method, NaOH erosion method, and balanced salt incubation method, but they are less used than the first two methods.
The above preparation method is a general method for preparing acellular allogene

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hernia patch, preparation method and application thereof in herniorrhaphy
  • Hernia patch, preparation method and application thereof in herniorrhaphy
  • Hernia patch, preparation method and application thereof in herniorrhaphy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Preparation method of acellular allodermal matrix

[0063] Put the allogeneic leather raw material into the concentration of 0.2g / L phospholipase and 0.1g / L trypsin solution, the pH value of the enzyme solution is 7.0, the temperature is 37°C, shake at 100rpm for 2 hours, replace the enzyme solution once, repeat this process once, The semi-finished product A is obtained; the phospholipase is a mixture of phospholipase A1, phospholipase A2, phospholipase C and phospholipase D according to the mass ratio of 1:1:1:1.

[0064] The semi-finished product A was taken out, soaked in physiological saline, and oscillated 3 times, each time for 2 hours, with an oscillating speed of 80 rpm and a temperature of 2°C to obtain a semi-finished product B.

[0065] Put the semi-finished product B into the TritonX-100 solution containing 0.2g / L, 50KHz, 220W, ultrasonic for 5 minutes, soak for 3 hours, repeat this process 3 times, and get the semi-finished product C.

[0066] Th...

Embodiment 2

[0076] Example 2 Preparation method of acellular allogeneic dermal matrix

[0077] Put allogeneic leather raw materials into 0.1g / L phospholipase and 0.1g / L trypsin solution, the pH value of the enzyme solution is 7.0, the temperature is 37°C, shake at 100rpm for 2 hours, replace the enzyme solution once, repeat this process once, The semi-finished product A is obtained; the phospholipase is a mixture of phospholipase A1, phospholipase A2, phospholipase C and phospholipase D according to the mass ratio of 1:1:1:1.

[0078] The semi-finished product A was taken out, put into a container filled with physiological saline solution, soaked in physiological saline, oscillated 3 times, each time for 2 hours, the oscillation speed was 80 rpm, and the temperature was 2°C to obtain the semi-finished product B.

[0079] Put the semi-finished product B into the SDS solution containing 0.2g / L, 10mM EDTA, 50KHz, 220W, ultrasonic for 7 minutes, soak for 2 hours, repeat this process 3 times, ...

experiment example 1

[0089] Experimental example 1 Detection of biological properties of acellular allogeneic dermal matrix

[0090] Biological properties such as the mechanical characteristics of the acellular allogeneic dermal matrix (i.e. finished product K, hernia patch) prepared in Example 1-2 and the acellular allogenic dermal matrix (control group) produced by a domestic enterprise are tested, and the performance index Including tensile strength, suture strength, bursting strength, tensile elongation, water content, and DNA residue. The specific test results are shown in Table 1. The results show that the product of the invention has good toughness and elasticity, is convenient for suturing and pulling, and has sufficient tensile strength, which can meet the treatment needs of hernia repair patch.

[0091] Table 1 Test results after rehydration of acellular allogeneic dermal matrix

[0092] Acellular Allodermal Matrix

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Tensile strengthaaaaaaaaaa
Suture strengthaaaaaaaaaa
Bursting strengthaaaaaaaaaa
Login to view more

Abstract

The invention relates to a hernia patch, a preparation method and application thereof in herniorrhaphy. The hernia patch is prepared by completely removing all cells possibly causing host immunological rejection from an allogenic skin raw material and integrallty retaining extracellular matrix with the same original tissue structure. The acellular allogenic dermal matrix has tensile strength of 3.0MPa-7.0MPa, stitching strength of 18N-22N, tensile elongation of 10%-30% and bursting strength of 20KPa-30KPa. The hernia patch provided by the invention has the effect of inducing tissue generation,and can be recognized as autologous tissue by human tissue cells after implantation, and soon new blood vessels and fibroblasts can grow into the patch to guide orderly growth of cells along the collagen frame, thus reaching the purpose of supplementation, especially fast repair. The patch is non-degradable within 24 weeks after filling.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biomedical materials, and in particular relates to a hernia patch, its preparation method and its application in hernia repair. Background technique [0002] A hernia is any organ or tissue that leaves its original location and enters another site through a normal or abnormal congenital or acquired weak point, defect or hole in the body. Hernia can be divided into direct inguinal hernia, indirect inguinal hernia, femoral hernia, umbilical hernia, linea alba hernia and incisional hernia according to its location. General symptoms: prominent when standing, disappear after lying on the back, and can return to the abdominal cavity when pressed. Hernia first affects patient's digestive system, thereby occurs the symptoms such as hypogastric region pendant bulge, flatulence, stomachache, constipation, alimentary absorption function is poor, fatiguability and body constitution decline. An...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L27/36
CPCA61L27/362A61L27/3687A61L27/3691A61L27/3695A61L2430/40
Inventor 孙继煌石清东
Owner BEIJING JAYYALIFE BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap